Experiment: MoYLS4 with Rubidium Chloride 100mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pleD and pep* are separated by 351 nucleotides pep* and fur are separated by 76 nucleotides
DVU0940: pleD - GGDEF domain protein (TIGR), at 1,030,439 to 1,031,170
pleD
DVU0941: pep* - metalloprotease, iron-related (Dmitry Rodionov), at 1,031,522 to 1,034,416
pep*
DVU0942: fur - ferric uptake regulator (Dmitry Rodionov), at 1,034,493 to 1,034,927
fur
Position (kb)
1031
1032
1033
1034
1035 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1030.552 kb on - strand, within pleD at 1030.608 kb on + strand, within pleD at 1030.609 kb on + strand, within pleD at 1030.647 kb on + strand, within pleD at 1030.652 kb on + strand, within pleD at 1030.708 kb on - strand, within pleD at 1030.773 kb on - strand, within pleD at 1030.780 kb on + strand, within pleD at 1030.780 kb on + strand, within pleD at 1030.873 kb on + strand, within pleD at 1030.875 kb on + strand, within pleD at 1030.876 kb on - strand, within pleD at 1030.881 kb on - strand, within pleD at 1030.907 kb on - strand, within pleD at 1030.909 kb on + strand, within pleD at 1030.917 kb on - strand, within pleD at 1030.917 kb on - strand, within pleD at 1030.917 kb on - strand, within pleD at 1031.052 kb on - strand, within pleD at 1031.066 kb on + strand, within pleD at 1031.117 kb on + strand at 1031.117 kb on + strand at 1031.117 kb on + strand at 1031.125 kb on - strand at 1031.125 kb on - strand at 1031.125 kb on - strand at 1031.125 kb on - strand at 1031.125 kb on - strand at 1031.125 kb on - strand at 1031.125 kb on - strand at 1031.151 kb on + strand at 1031.160 kb on + strand at 1031.221 kb on + strand at 1031.368 kb on - strand at 1031.512 kb on + strand at 1031.520 kb on + strand at 1031.543 kb on - strand at 1031.600 kb on + strand at 1031.675 kb on + strand at 1031.675 kb on + strand at 1031.686 kb on + strand at 1031.770 kb on + strand at 1031.778 kb on - strand at 1031.778 kb on - strand at 1031.814 kb on - strand, within pep* at 1032.091 kb on + strand, within pep* at 1032.288 kb on + strand, within pep* at 1032.332 kb on + strand, within pep* at 1032.425 kb on + strand, within pep* at 1032.435 kb on - strand, within pep* at 1032.436 kb on + strand, within pep* at 1032.461 kb on - strand, within pep* at 1032.495 kb on + strand, within pep* at 1032.574 kb on + strand, within pep* at 1032.606 kb on + strand, within pep* at 1032.614 kb on + strand, within pep* at 1032.647 kb on + strand, within pep* at 1032.661 kb on + strand, within pep* at 1032.748 kb on - strand, within pep* at 1032.882 kb on - strand, within pep* at 1032.936 kb on + strand, within pep* at 1033.018 kb on - strand, within pep* at 1033.087 kb on - strand, within pep* at 1033.215 kb on - strand, within pep* at 1033.356 kb on + strand, within pep* at 1033.389 kb on - strand, within pep* at 1033.392 kb on + strand, within pep* at 1033.499 kb on + strand, within pep* at 1033.609 kb on + strand, within pep* at 1033.621 kb on + strand, within pep* at 1033.680 kb on - strand, within pep* at 1033.715 kb on + strand, within pep* at 1033.789 kb on + strand, within pep* at 1033.897 kb on + strand, within pep* at 1033.966 kb on - strand, within pep* at 1033.966 kb on - strand, within pep* at 1034.084 kb on - strand, within pep* at 1034.084 kb on - strand, within pep* at 1034.123 kb on - strand, within pep* at 1034.334 kb on + strand at 1034.340 kb on + strand at 1034.450 kb on + strand at 1034.463 kb on - strand at 1034.934 kb on - strand at 1034.937 kb on + strand at 1034.957 kb on - strand at 1035.017 kb on + strand at 1035.101 kb on + strand at 1035.109 kb on - strand at 1035.124 kb on - strand at 1035.158 kb on + strand at 1035.196 kb on + strand at 1035.199 kb on - strand at 1035.259 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Rubidium Chloride 100mM remove 1,030,552 - pleD DVU0940 0.15 +0.5 1,030,608 + pleD DVU0940 0.23 -0.4 1,030,609 + pleD DVU0940 0.23 +0.5 1,030,647 + pleD DVU0940 0.28 -0.2 1,030,652 + pleD DVU0940 0.29 -0.3 1,030,708 - pleD DVU0940 0.37 -2.5 1,030,773 - pleD DVU0940 0.46 -0.1 1,030,780 + pleD DVU0940 0.47 -0.7 1,030,780 + pleD DVU0940 0.47 +0.6 1,030,873 + pleD DVU0940 0.59 +0.5 1,030,875 + pleD DVU0940 0.60 +0.4 1,030,876 - pleD DVU0940 0.60 -0.1 1,030,881 - pleD DVU0940 0.60 -0.1 1,030,907 - pleD DVU0940 0.64 +0.2 1,030,909 + pleD DVU0940 0.64 +0.7 1,030,917 - pleD DVU0940 0.65 +0.3 1,030,917 - pleD DVU0940 0.65 +1.4 1,030,917 - pleD DVU0940 0.65 +1.6 1,031,052 - pleD DVU0940 0.84 -0.2 1,031,066 + pleD DVU0940 0.86 -1.2 1,031,117 + -0.5 1,031,117 + -1.0 1,031,117 + +0.2 1,031,125 - +0.2 1,031,125 - +0.0 1,031,125 - -0.0 1,031,125 - -0.2 1,031,125 - +0.1 1,031,125 - -0.1 1,031,125 - +0.7 1,031,151 + +1.3 1,031,160 + -0.3 1,031,221 + -0.4 1,031,368 - -0.1 1,031,512 + +0.3 1,031,520 + +0.3 1,031,543 - +2.9 1,031,600 + -0.2 1,031,675 + +1.0 1,031,675 + +1.6 1,031,686 + +0.3 1,031,770 + -1.2 1,031,778 - -0.9 1,031,778 - -0.9 1,031,814 - pep* DVU0941 0.10 -0.9 1,032,091 + pep* DVU0941 0.20 -1.4 1,032,288 + pep* DVU0941 0.26 -0.4 1,032,332 + pep* DVU0941 0.28 +0.1 1,032,425 + pep* DVU0941 0.31 -0.9 1,032,435 - pep* DVU0941 0.32 -0.9 1,032,436 + pep* DVU0941 0.32 +0.8 1,032,461 - pep* DVU0941 0.32 +0.7 1,032,495 + pep* DVU0941 0.34 -0.4 1,032,574 + pep* DVU0941 0.36 +0.6 1,032,606 + pep* DVU0941 0.37 -0.7 1,032,614 + pep* DVU0941 0.38 +1.0 1,032,647 + pep* DVU0941 0.39 -1.3 1,032,661 + pep* DVU0941 0.39 -0.9 1,032,748 - pep* DVU0941 0.42 -0.7 1,032,882 - pep* DVU0941 0.47 -0.4 1,032,936 + pep* DVU0941 0.49 -1.0 1,033,018 - pep* DVU0941 0.52 -1.1 1,033,087 - pep* DVU0941 0.54 -1.8 1,033,215 - pep* DVU0941 0.58 +2.6 1,033,356 + pep* DVU0941 0.63 -1.1 1,033,389 - pep* DVU0941 0.64 -0.4 1,033,392 + pep* DVU0941 0.65 +1.0 1,033,499 + pep* DVU0941 0.68 -1.4 1,033,609 + pep* DVU0941 0.72 +0.2 1,033,621 + pep* DVU0941 0.73 -1.8 1,033,680 - pep* DVU0941 0.75 -2.3 1,033,715 + pep* DVU0941 0.76 -0.4 1,033,789 + pep* DVU0941 0.78 -0.4 1,033,897 + pep* DVU0941 0.82 +0.6 1,033,966 - pep* DVU0941 0.84 -0.9 1,033,966 - pep* DVU0941 0.84 -1.0 1,034,084 - pep* DVU0941 0.88 +1.0 1,034,084 - pep* DVU0941 0.88 +0.9 1,034,123 - pep* DVU0941 0.90 +3.6 1,034,334 + -2.5 1,034,340 + -1.4 1,034,450 + +0.3 1,034,463 - +0.1 1,034,934 - -0.9 1,034,937 + -2.5 1,034,957 - -0.1 1,035,017 + -0.4 1,035,101 + +0.6 1,035,109 - +0.6 1,035,124 - +0.3 1,035,158 + -0.7 1,035,196 + -0.0 1,035,199 - +0.4 1,035,259 + +0.3
Or see this region's nucleotide sequence