Experiment: MoYLS4 with Cobalt Chloride 20uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0054 and DVUA0055 overlap by 4 nucleotides DVUA0055 and DVUA0056 overlap by 11 nucleotides DVUA0056 and atoC are separated by 154 nucleotides
DVUA0054: DVUA0054 - glycosyl transferase, group 1 family protein (TIGR), at 65,572 to 66,972
DVUA0054
DVUA0055: DVUA0055 - membrane protein, putative (TIGR), at 66,969 to 67,922
DVUA0055
DVUA0056: DVUA0056 - hypothetical protein (TIGR), at 67,912 to 68,532
DVUA0056
DVUA0057: atoC - sigma-54 dependent transcriptional regulator/response regulator (TIGR), at 68,687 to 70,117
atoC
Position (kb) 67
68
69 Strain fitness (log2 ratio) -1
0
1
2
3 at 66.921 kb on + strand at 67.007 kb on - strand at 67.061 kb on + strand at 67.102 kb on + strand, within DVUA0055 at 67.102 kb on + strand, within DVUA0055 at 67.105 kb on + strand, within DVUA0055 at 67.108 kb on - strand, within DVUA0055 at 67.117 kb on + strand, within DVUA0055 at 67.125 kb on - strand, within DVUA0055 at 67.137 kb on + strand, within DVUA0055 at 67.172 kb on - strand, within DVUA0055 at 67.231 kb on + strand, within DVUA0055 at 67.231 kb on + strand, within DVUA0055 at 67.239 kb on - strand, within DVUA0055 at 67.239 kb on - strand, within DVUA0055 at 67.275 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.378 kb on - strand, within DVUA0055 at 67.378 kb on - strand, within DVUA0055 at 67.446 kb on + strand, within DVUA0055 at 67.651 kb on + strand, within DVUA0055 at 67.803 kb on + strand, within DVUA0055 at 67.803 kb on + strand, within DVUA0055 at 67.874 kb on + strand at 67.892 kb on - strand at 67.911 kb on + strand at 67.966 kb on + strand at 67.974 kb on - strand, within DVUA0056 at 68.040 kb on + strand, within DVUA0056 at 68.045 kb on + strand, within DVUA0056 at 68.045 kb on + strand, within DVUA0056 at 68.053 kb on - strand, within DVUA0056 at 68.053 kb on - strand, within DVUA0056 at 68.053 kb on - strand, within DVUA0056 at 68.053 kb on - strand, within DVUA0056 at 68.143 kb on - strand, within DVUA0056 at 68.175 kb on + strand, within DVUA0056 at 68.181 kb on + strand, within DVUA0056 at 68.209 kb on - strand, within DVUA0056 at 68.212 kb on + strand, within DVUA0056 at 68.258 kb on - strand, within DVUA0056 at 68.427 kb on + strand, within DVUA0056 at 68.432 kb on + strand, within DVUA0056 at 68.450 kb on - strand, within DVUA0056 at 68.688 kb on + strand at 68.703 kb on - strand at 68.816 kb on - strand at 68.920 kb on + strand, within atoC at 69.123 kb on - strand, within atoC at 69.221 kb on + strand, within atoC at 69.231 kb on - strand, within atoC at 69.231 kb on - strand, within atoC at 69.231 kb on - strand, within atoC at 69.353 kb on + strand, within atoC at 69.368 kb on - strand, within atoC at 69.415 kb on - strand, within atoC at 69.424 kb on - strand, within atoC at 69.432 kb on - strand, within atoC at 69.443 kb on + strand, within atoC at 69.448 kb on + strand, within atoC at 69.448 kb on + strand, within atoC at 69.451 kb on - strand, within atoC at 69.489 kb on - strand, within atoC at 69.514 kb on + strand, within atoC
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Cobalt Chloride 20uM remove 66,921 + +2.0 67,007 - +1.0 67,061 + -0.7 67,102 + DVUA0055 0.14 -0.8 67,102 + DVUA0055 0.14 +3.0 67,105 + DVUA0055 0.14 -0.0 67,108 - DVUA0055 0.15 +0.1 67,117 + DVUA0055 0.16 +0.0 67,125 - DVUA0055 0.16 -0.6 67,137 + DVUA0055 0.18 +0.2 67,172 - DVUA0055 0.21 +1.0 67,231 + DVUA0055 0.27 +1.7 67,231 + DVUA0055 0.27 -0.2 67,239 - DVUA0055 0.28 -0.5 67,239 - DVUA0055 0.28 -0.4 67,275 + DVUA0055 0.32 +0.4 67,370 + DVUA0055 0.42 -0.2 67,370 + DVUA0055 0.42 +0.8 67,370 + DVUA0055 0.42 +0.2 67,370 + DVUA0055 0.42 -0.5 67,378 - DVUA0055 0.43 +0.2 67,378 - DVUA0055 0.43 +0.5 67,446 + DVUA0055 0.50 +1.4 67,651 + DVUA0055 0.71 +0.4 67,803 + DVUA0055 0.87 +0.2 67,803 + DVUA0055 0.87 -0.8 67,874 + -0.5 67,892 - -0.0 67,911 + -0.0 67,966 + -1.0 67,974 - DVUA0056 0.10 +0.5 68,040 + DVUA0056 0.21 +0.3 68,045 + DVUA0056 0.21 +1.1 68,045 + DVUA0056 0.21 -0.5 68,053 - DVUA0056 0.23 -0.1 68,053 - DVUA0056 0.23 -0.2 68,053 - DVUA0056 0.23 +0.2 68,053 - DVUA0056 0.23 +0.4 68,143 - DVUA0056 0.37 +0.2 68,175 + DVUA0056 0.42 -0.1 68,181 + DVUA0056 0.43 +0.1 68,209 - DVUA0056 0.48 -0.1 68,212 + DVUA0056 0.48 -0.0 68,258 - DVUA0056 0.56 +2.0 68,427 + DVUA0056 0.83 -0.9 68,432 + DVUA0056 0.84 +0.4 68,450 - DVUA0056 0.87 -0.3 68,688 + +0.9 68,703 - -0.7 68,816 - -0.4 68,920 + atoC DVUA0057 0.16 +0.5 69,123 - atoC DVUA0057 0.30 +0.1 69,221 + atoC DVUA0057 0.37 +2.0 69,231 - atoC DVUA0057 0.38 +0.3 69,231 - atoC DVUA0057 0.38 -0.8 69,231 - atoC DVUA0057 0.38 -0.3 69,353 + atoC DVUA0057 0.47 -1.1 69,368 - atoC DVUA0057 0.48 -0.6 69,415 - atoC DVUA0057 0.51 -1.2 69,424 - atoC DVUA0057 0.52 -0.0 69,432 - atoC DVUA0057 0.52 -0.7 69,443 + atoC DVUA0057 0.53 +2.0 69,448 + atoC DVUA0057 0.53 -0.8 69,448 + atoC DVUA0057 0.53 -0.6 69,451 - atoC DVUA0057 0.53 +2.0 69,489 - atoC DVUA0057 0.56 -0.6 69,514 + atoC DVUA0057 0.58 -0.6
Or see this region's nucleotide sequence