Experiment: MoYLS4 with Cobalt Chloride 20uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppsA and tRNA-Leu are separated by 178 nucleotides tRNA-Leu and DVU0153 are separated by 191 nucleotides DVU0153 and DVU0155 are separated by 467 nucleotides
DVU0152: ppsA - phosphoenolpyruvate synthase-related protein (TIGR), at 186,986 to 189,547
ppsA
DVU_tRNA-Leu-1: tRNA-Leu - tRNA with anticodon TAA for Leu (VIMSS-AUTO), at 189,726 to 189,812
tRNA-Leu
DVU0153: DVU0153 - hypothetical protein (TIGR), at 190,004 to 190,387
DVU0153
DVU0155: DVU0155 - type I phosphodiesterase/nucleotide pyrophosphatase family protein (TIGR), at 190,855 to 192,243
DVU0155
Position (kb) 190
191 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 189.012 kb on - strand, within ppsA at 189.017 kb on + strand, within ppsA at 189.031 kb on + strand, within ppsA at 189.039 kb on - strand, within ppsA at 189.048 kb on + strand, within ppsA at 189.056 kb on - strand, within ppsA at 189.225 kb on - strand, within ppsA at 189.251 kb on + strand, within ppsA at 189.328 kb on + strand at 189.344 kb on - strand at 189.379 kb on - strand at 189.392 kb on - strand at 189.394 kb on - strand at 189.462 kb on - strand at 189.668 kb on - strand at 189.669 kb on - strand at 189.842 kb on + strand at 189.842 kb on + strand at 189.884 kb on - strand at 189.889 kb on + strand at 189.897 kb on + strand at 189.931 kb on + strand at 189.938 kb on + strand at 189.946 kb on - strand at 189.946 kb on - strand at 189.951 kb on - strand at 189.956 kb on - strand at 190.000 kb on - strand at 190.062 kb on + strand, within DVU0153 at 190.091 kb on + strand, within DVU0153 at 190.091 kb on - strand, within DVU0153 at 190.093 kb on + strand, within DVU0153 at 190.098 kb on + strand, within DVU0153 at 190.123 kb on + strand, within DVU0153 at 190.129 kb on - strand, within DVU0153 at 190.158 kb on - strand, within DVU0153 at 190.172 kb on - strand, within DVU0153 at 190.176 kb on + strand, within DVU0153 at 190.176 kb on + strand, within DVU0153 at 190.181 kb on + strand, within DVU0153 at 190.184 kb on - strand, within DVU0153 at 190.189 kb on - strand, within DVU0153 at 190.189 kb on - strand, within DVU0153 at 190.189 kb on - strand, within DVU0153 at 190.235 kb on - strand, within DVU0153 at 190.247 kb on + strand, within DVU0153 at 190.255 kb on - strand, within DVU0153 at 190.256 kb on + strand, within DVU0153 at 190.270 kb on - strand, within DVU0153 at 190.270 kb on - strand, within DVU0153 at 190.290 kb on + strand, within DVU0153 at 190.306 kb on - strand, within DVU0153 at 190.330 kb on + strand, within DVU0153 at 190.373 kb on + strand at 190.501 kb on + strand at 190.557 kb on + strand at 190.557 kb on + strand at 190.571 kb on - strand at 190.593 kb on + strand at 190.627 kb on - strand at 190.671 kb on - strand at 190.707 kb on + strand at 190.809 kb on + strand at 190.819 kb on - strand at 190.830 kb on - strand at 190.878 kb on - strand at 190.900 kb on - strand at 190.914 kb on - strand at 190.934 kb on + strand at 190.956 kb on - strand at 191.034 kb on + strand, within DVU0155 at 191.040 kb on - strand, within DVU0155 at 191.042 kb on - strand, within DVU0155 at 191.042 kb on - strand, within DVU0155 at 191.042 kb on - strand, within DVU0155 at 191.044 kb on + strand, within DVU0155 at 191.104 kb on + strand, within DVU0155 at 191.104 kb on + strand, within DVU0155 at 191.109 kb on + strand, within DVU0155 at 191.109 kb on + strand, within DVU0155 at 191.109 kb on + strand, within DVU0155 at 191.117 kb on - strand, within DVU0155 at 191.196 kb on + strand, within DVU0155 at 191.204 kb on - strand, within DVU0155 at 191.210 kb on - strand, within DVU0155 at 191.242 kb on + strand, within DVU0155 at 191.282 kb on - strand, within DVU0155
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Cobalt Chloride 20uM remove 189,012 - ppsA DVU0152 0.79 -0.5 189,017 + ppsA DVU0152 0.79 -0.6 189,031 + ppsA DVU0152 0.80 +0.2 189,039 - ppsA DVU0152 0.80 +0.7 189,048 + ppsA DVU0152 0.80 -0.3 189,056 - ppsA DVU0152 0.81 +0.1 189,225 - ppsA DVU0152 0.87 +2.9 189,251 + ppsA DVU0152 0.88 -0.7 189,328 + -1.1 189,344 - -0.3 189,379 - -0.7 189,392 - -0.1 189,394 - +0.1 189,462 - -0.5 189,668 - -0.1 189,669 - -0.4 189,842 + +0.9 189,842 + -1.0 189,884 - -0.7 189,889 + +0.2 189,897 + -0.5 189,931 + +1.9 189,938 + +0.0 189,946 - -0.2 189,946 - +1.9 189,951 - -0.4 189,956 - -0.1 190,000 - +0.7 190,062 + DVU0153 0.15 -0.7 190,091 + DVU0153 0.23 -0.3 190,091 - DVU0153 0.23 +0.5 190,093 + DVU0153 0.23 -0.3 190,098 + DVU0153 0.24 +0.1 190,123 + DVU0153 0.31 +0.1 190,129 - DVU0153 0.33 -0.0 190,158 - DVU0153 0.40 -0.4 190,172 - DVU0153 0.44 -0.4 190,176 + DVU0153 0.45 +0.0 190,176 + DVU0153 0.45 +1.3 190,181 + DVU0153 0.46 +0.7 190,184 - DVU0153 0.47 -1.2 190,189 - DVU0153 0.48 +1.0 190,189 - DVU0153 0.48 +0.0 190,189 - DVU0153 0.48 +0.7 190,235 - DVU0153 0.60 -0.1 190,247 + DVU0153 0.63 +0.1 190,255 - DVU0153 0.65 +1.2 190,256 + DVU0153 0.66 -0.3 190,270 - DVU0153 0.69 -0.8 190,270 - DVU0153 0.69 +1.1 190,290 + DVU0153 0.74 +0.5 190,306 - DVU0153 0.79 +0.1 190,330 + DVU0153 0.85 -0.0 190,373 + -0.3 190,501 + -0.1 190,557 + +0.4 190,557 + -0.9 190,571 - -0.3 190,593 + -0.6 190,627 - -1.4 190,671 - -0.5 190,707 + -0.7 190,809 + -2.1 190,819 - -0.4 190,830 - -0.7 190,878 - -1.2 190,900 - +0.4 190,914 - +0.7 190,934 + +1.1 190,956 - -0.7 191,034 + DVU0155 0.13 +1.6 191,040 - DVU0155 0.13 -0.8 191,042 - DVU0155 0.13 -0.2 191,042 - DVU0155 0.13 +1.3 191,042 - DVU0155 0.13 +0.0 191,044 + DVU0155 0.14 -0.1 191,104 + DVU0155 0.18 -1.5 191,104 + DVU0155 0.18 +0.0 191,109 + DVU0155 0.18 -0.1 191,109 + DVU0155 0.18 -1.4 191,109 + DVU0155 0.18 -2.8 191,117 - DVU0155 0.19 -0.9 191,196 + DVU0155 0.25 +1.5 191,204 - DVU0155 0.25 -0.0 191,210 - DVU0155 0.26 +0.5 191,242 + DVU0155 0.28 -1.5 191,282 - DVU0155 0.31 +0.4
Or see this region's nucleotide sequence