Experiment: MoYLS4 with Cobalt Chloride 20uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hspC and DVU2442 are separated by 60 nucleotides DVU2442 and DVU2443 are separated by 225 nucleotides DVU2443 and flaB3 are separated by 180 nucleotides
DVU2441: hspC - heat shock protein, Hsp20 family (TIGR), at 2,547,504 to 2,547,914
hspC
DVU2442: DVU2442 - heat shock protein, Hsp20 family (TIGR), at 2,547,975 to 2,548,328
DVU2442
DVU2443: DVU2443 - conserved hypothetical protein (TIGR), at 2,548,554 to 2,549,180
DVU2443
DVU2444: flaB3 - flagellin (TIGR), at 2,549,361 to 2,550,254
flaB3
Position (kb)
2548
2549
2550 Strain fitness (log2 ratio)
-1
0
1
2 at 2547.872 kb on + strand, within hspC at 2547.888 kb on - strand at 2547.908 kb on + strand at 2548.162 kb on - strand, within DVU2442 at 2548.201 kb on + strand, within DVU2442 at 2548.209 kb on + strand, within DVU2442 at 2548.267 kb on + strand, within DVU2442 at 2548.321 kb on + strand at 2548.323 kb on + strand at 2548.331 kb on - strand at 2548.331 kb on - strand at 2548.331 kb on - strand at 2548.331 kb on - strand at 2548.331 kb on - strand at 2548.350 kb on + strand at 2548.443 kb on - strand at 2548.481 kb on - strand at 2548.568 kb on + strand at 2548.581 kb on - strand at 2548.582 kb on - strand at 2548.590 kb on + strand at 2548.603 kb on + strand at 2548.618 kb on + strand, within DVU2443 at 2548.618 kb on + strand, within DVU2443 at 2548.666 kb on + strand, within DVU2443 at 2548.666 kb on + strand, within DVU2443 at 2548.672 kb on - strand, within DVU2443 at 2548.707 kb on - strand, within DVU2443 at 2548.714 kb on - strand, within DVU2443 at 2548.770 kb on - strand, within DVU2443 at 2548.799 kb on + strand, within DVU2443 at 2548.895 kb on + strand, within DVU2443 at 2548.958 kb on - strand, within DVU2443 at 2548.963 kb on - strand, within DVU2443 at 2548.965 kb on - strand, within DVU2443 at 2549.070 kb on - strand, within DVU2443 at 2549.195 kb on - strand at 2549.262 kb on + strand at 2549.321 kb on - strand at 2549.370 kb on + strand at 2549.421 kb on - strand at 2549.523 kb on - strand, within flaB3 at 2549.762 kb on - strand, within flaB3 at 2549.836 kb on + strand, within flaB3 at 2549.846 kb on + strand, within flaB3 at 2549.947 kb on - strand, within flaB3 at 2550.064 kb on + strand, within flaB3 at 2550.175 kb on + strand at 2550.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Cobalt Chloride 20uM remove 2,547,872 + hspC DVU2441 0.90 +0.2 2,547,888 - -0.6 2,547,908 + -0.4 2,548,162 - DVU2442 0.53 -0.4 2,548,201 + DVU2442 0.64 -0.3 2,548,209 + DVU2442 0.66 -0.5 2,548,267 + DVU2442 0.82 +0.1 2,548,321 + +0.9 2,548,323 + -0.6 2,548,331 - -0.0 2,548,331 - -0.6 2,548,331 - -1.0 2,548,331 - -0.5 2,548,331 - -0.8 2,548,350 + +0.4 2,548,443 - +0.1 2,548,481 - +2.0 2,548,568 + +1.7 2,548,581 - -0.6 2,548,582 - +0.4 2,548,590 + -0.6 2,548,603 + -0.3 2,548,618 + DVU2443 0.10 -0.2 2,548,618 + DVU2443 0.10 -0.1 2,548,666 + DVU2443 0.18 +0.8 2,548,666 + DVU2443 0.18 +0.7 2,548,672 - DVU2443 0.19 -0.3 2,548,707 - DVU2443 0.24 +0.5 2,548,714 - DVU2443 0.26 -0.2 2,548,770 - DVU2443 0.34 +2.4 2,548,799 + DVU2443 0.39 -1.3 2,548,895 + DVU2443 0.54 -0.3 2,548,958 - DVU2443 0.64 +0.2 2,548,963 - DVU2443 0.65 +0.9 2,548,965 - DVU2443 0.66 +1.4 2,549,070 - DVU2443 0.82 -0.4 2,549,195 - +1.6 2,549,262 + +0.1 2,549,321 - +1.0 2,549,370 + +1.0 2,549,421 - -0.1 2,549,523 - flaB3 DVU2444 0.18 +0.4 2,549,762 - flaB3 DVU2444 0.45 -0.0 2,549,836 + flaB3 DVU2444 0.53 -0.4 2,549,846 + flaB3 DVU2444 0.54 -0.7 2,549,947 - flaB3 DVU2444 0.66 +0.6 2,550,064 + flaB3 DVU2444 0.79 -0.2 2,550,175 + -0.1 2,550,180 - -1.3
Or see this region's nucleotide sequence