Experiment: MoLS4 with L-Cysteine 5mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cas3 and DVUA0130 are separated by 13 nucleotides DVUA0130 and DVUA0131 overlap by 4 nucleotides DVUA0131 and DVUA0132 are separated by 2 nucleotides DVUA0132 and DVUA0133 are separated by 1 nucleotides
DVUA0129: cas3 - CRISPR-associated helicase Cas3 domain protein (TIGR), at 168,254 to 170,362
cas3
DVUA0130: DVUA0130 - CRISPR-associated protein, CT1134 family (TIGR), at 170,376 to 171,059
DVUA0130
DVUA0131: DVUA0131 - CRISPR-associated protein, CT1133 family (TIGR), at 171,056 to 172,894
DVUA0131
DVUA0132: DVUA0132 - CRISPR-associated protein, TM1801 family (TIGR), at 172,897 to 173,769
DVUA0132
DVUA0133: DVUA0133 - CRISPR-associated protein Cas4 (TIGR), at 173,771 to 174,409
DVUA0133
Position (kb) 171
172
173 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 170.115 kb on + strand, within cas3 at 170.123 kb on + strand, within cas3 at 170.166 kb on + strand at 170.166 kb on + strand at 170.166 kb on + strand at 170.174 kb on - strand at 170.174 kb on - strand at 170.179 kb on - strand at 170.181 kb on + strand at 170.268 kb on + strand at 170.304 kb on + strand at 170.333 kb on - strand at 170.404 kb on + strand at 170.425 kb on - strand at 170.489 kb on - strand, within DVUA0130 at 170.509 kb on + strand, within DVUA0130 at 170.510 kb on + strand, within DVUA0130 at 170.517 kb on - strand, within DVUA0130 at 170.560 kb on + strand, within DVUA0130 at 170.565 kb on - strand, within DVUA0130 at 170.702 kb on - strand, within DVUA0130 at 170.763 kb on + strand, within DVUA0130 at 170.796 kb on + strand, within DVUA0130 at 170.959 kb on - strand, within DVUA0130 at 171.135 kb on + strand at 171.153 kb on - strand at 171.355 kb on + strand, within DVUA0131 at 171.363 kb on - strand, within DVUA0131 at 171.379 kb on + strand, within DVUA0131 at 171.387 kb on - strand, within DVUA0131 at 171.404 kb on + strand, within DVUA0131 at 171.532 kb on + strand, within DVUA0131 at 171.585 kb on + strand, within DVUA0131 at 171.607 kb on - strand, within DVUA0131 at 171.607 kb on - strand, within DVUA0131 at 171.612 kb on + strand, within DVUA0131 at 171.655 kb on + strand, within DVUA0131 at 171.663 kb on - strand, within DVUA0131 at 171.712 kb on - strand, within DVUA0131 at 171.789 kb on + strand, within DVUA0131 at 171.800 kb on + strand, within DVUA0131 at 171.800 kb on + strand, within DVUA0131 at 171.838 kb on + strand, within DVUA0131 at 171.865 kb on + strand, within DVUA0131 at 171.873 kb on - strand, within DVUA0131 at 171.888 kb on - strand, within DVUA0131 at 171.893 kb on + strand, within DVUA0131 at 171.930 kb on - strand, within DVUA0131 at 171.991 kb on + strand, within DVUA0131 at 172.079 kb on - strand, within DVUA0131 at 172.089 kb on + strand, within DVUA0131 at 172.118 kb on - strand, within DVUA0131 at 172.184 kb on + strand, within DVUA0131 at 172.189 kb on + strand, within DVUA0131 at 172.294 kb on + strand, within DVUA0131 at 172.336 kb on + strand, within DVUA0131 at 172.336 kb on + strand, within DVUA0131 at 172.344 kb on - strand, within DVUA0131 at 172.407 kb on - strand, within DVUA0131 at 172.414 kb on + strand, within DVUA0131 at 172.422 kb on - strand, within DVUA0131 at 172.556 kb on - strand, within DVUA0131 at 172.575 kb on - strand, within DVUA0131 at 172.625 kb on + strand, within DVUA0131 at 172.831 kb on + strand at 172.848 kb on - strand at 172.863 kb on - strand at 173.031 kb on - strand, within DVUA0132 at 173.086 kb on - strand, within DVUA0132 at 173.139 kb on + strand, within DVUA0132 at 173.232 kb on + strand, within DVUA0132 at 173.268 kb on - strand, within DVUA0132 at 173.268 kb on - strand, within DVUA0132 at 173.274 kb on + strand, within DVUA0132 at 173.274 kb on + strand, within DVUA0132 at 173.282 kb on - strand, within DVUA0132 at 173.490 kb on + strand, within DVUA0132 at 173.523 kb on + strand, within DVUA0132 at 173.523 kb on + strand, within DVUA0132 at 173.523 kb on + strand, within DVUA0132 at 173.523 kb on + strand, within DVUA0132 at 173.531 kb on - strand, within DVUA0132 at 173.531 kb on - strand, within DVUA0132 at 173.611 kb on + strand, within DVUA0132 at 173.672 kb on - strand, within DVUA0132 at 173.723 kb on + strand at 173.736 kb on - strand at 173.744 kb on + strand at 173.751 kb on + strand at 173.816 kb on + strand at 173.821 kb on + strand at 173.821 kb on + strand at 173.871 kb on - strand, within DVUA0133 at 173.876 kb on + strand, within DVUA0133 at 173.889 kb on - strand, within DVUA0133 at 173.889 kb on - strand, within DVUA0133
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine 5mM as nutrient remove 170,115 + cas3 DVUA0129 0.88 +0.1 170,123 + cas3 DVUA0129 0.89 -0.6 170,166 + -0.7 170,166 + -0.8 170,166 + -0.2 170,174 - -0.9 170,174 - -0.4 170,179 - +0.9 170,181 + -0.7 170,268 + -1.0 170,304 + +0.0 170,333 - -0.2 170,404 + +0.4 170,425 - -0.3 170,489 - DVUA0130 0.17 +0.1 170,509 + DVUA0130 0.19 +0.1 170,510 + DVUA0130 0.20 +0.1 170,517 - DVUA0130 0.21 -0.8 170,560 + DVUA0130 0.27 -0.7 170,565 - DVUA0130 0.28 -2.2 170,702 - DVUA0130 0.48 +0.7 170,763 + DVUA0130 0.57 -0.7 170,796 + DVUA0130 0.61 -0.1 170,959 - DVUA0130 0.85 +0.2 171,135 + -0.2 171,153 - +0.5 171,355 + DVUA0131 0.16 -0.9 171,363 - DVUA0131 0.17 -0.1 171,379 + DVUA0131 0.18 -0.7 171,387 - DVUA0131 0.18 +0.3 171,404 + DVUA0131 0.19 -1.2 171,532 + DVUA0131 0.26 -0.3 171,585 + DVUA0131 0.29 -0.7 171,607 - DVUA0131 0.30 -0.7 171,607 - DVUA0131 0.30 -0.0 171,612 + DVUA0131 0.30 +0.2 171,655 + DVUA0131 0.33 +0.6 171,663 - DVUA0131 0.33 +0.2 171,712 - DVUA0131 0.36 -1.5 171,789 + DVUA0131 0.40 +0.7 171,800 + DVUA0131 0.40 -0.1 171,800 + DVUA0131 0.40 +0.2 171,838 + DVUA0131 0.43 +0.2 171,865 + DVUA0131 0.44 -0.5 171,873 - DVUA0131 0.44 -0.2 171,888 - DVUA0131 0.45 +0.4 171,893 + DVUA0131 0.46 -1.6 171,930 - DVUA0131 0.48 -0.3 171,991 + DVUA0131 0.51 -0.1 172,079 - DVUA0131 0.56 +0.4 172,089 + DVUA0131 0.56 +1.5 172,118 - DVUA0131 0.58 -0.4 172,184 + DVUA0131 0.61 -0.8 172,189 + DVUA0131 0.62 -1.3 172,294 + DVUA0131 0.67 -1.0 172,336 + DVUA0131 0.70 +1.5 172,336 + DVUA0131 0.70 +0.8 172,344 - DVUA0131 0.70 -0.5 172,407 - DVUA0131 0.73 -0.6 172,414 + DVUA0131 0.74 +0.3 172,422 - DVUA0131 0.74 -0.5 172,556 - DVUA0131 0.82 -0.7 172,575 - DVUA0131 0.83 +0.0 172,625 + DVUA0131 0.85 -2.2 172,831 + +0.5 172,848 - -0.0 172,863 - -0.3 173,031 - DVUA0132 0.15 +0.3 173,086 - DVUA0132 0.22 -0.0 173,139 + DVUA0132 0.28 +0.9 173,232 + DVUA0132 0.38 +0.2 173,268 - DVUA0132 0.42 +0.4 173,268 - DVUA0132 0.42 -1.1 173,274 + DVUA0132 0.43 -0.4 173,274 + DVUA0132 0.43 -0.2 173,282 - DVUA0132 0.44 +1.4 173,490 + DVUA0132 0.68 +0.6 173,523 + DVUA0132 0.72 -1.5 173,523 + DVUA0132 0.72 -0.1 173,523 + DVUA0132 0.72 +4.1 173,523 + DVUA0132 0.72 -0.6 173,531 - DVUA0132 0.73 -0.2 173,531 - DVUA0132 0.73 +0.4 173,611 + DVUA0132 0.82 -0.1 173,672 - DVUA0132 0.89 +0.1 173,723 + -0.8 173,736 - -0.3 173,744 + -1.0 173,751 + +0.9 173,816 + -0.1 173,821 + -0.3 173,821 + +0.9 173,871 - DVUA0133 0.16 -0.8 173,876 + DVUA0133 0.16 +0.5 173,889 - DVUA0133 0.18 -0.9 173,889 - DVUA0133 0.18 +0.0
Or see this region's nucleotide sequence