Experiment: MoLS4 with L-Cysteine 5mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and thiG are separated by 2 nucleotides thiG and thiS overlap by 1 nucleotides thiS and DVU2096 are separated by 525 nucleotides
DVU2093: thiH - thiH protein (TIGR), at 2,182,114 to 2,183,448
thiH
DVU2094: thiG - thiG protein (TIGR), at 2,183,451 to 2,184,248
thiG
DVU2095: thiS - thiamine biosynthesis protein ThiS (TIGR), at 2,184,248 to 2,184,448
thiS
DVU2096: DVU2096 - hypothetical protein (TIGR), at 2,184,974 to 2,185,066
DVU2096
Position (kb)
2183
2184
2185 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2182.474 kb on + strand, within thiH at 2182.853 kb on + strand, within thiH at 2182.878 kb on - strand, within thiH at 2182.887 kb on + strand, within thiH at 2183.038 kb on - strand, within thiH at 2183.038 kb on - strand, within thiH at 2183.055 kb on + strand, within thiH at 2183.063 kb on + strand, within thiH at 2183.120 kb on - strand, within thiH at 2183.125 kb on - strand, within thiH at 2183.159 kb on - strand, within thiH at 2183.193 kb on - strand, within thiH at 2183.195 kb on + strand, within thiH at 2183.220 kb on - strand, within thiH at 2183.222 kb on + strand, within thiH at 2183.229 kb on + strand, within thiH at 2183.229 kb on + strand, within thiH at 2183.237 kb on - strand, within thiH at 2183.284 kb on + strand, within thiH at 2183.344 kb on - strand at 2183.430 kb on + strand at 2183.502 kb on + strand at 2183.524 kb on - strand at 2183.525 kb on - strand at 2183.563 kb on + strand, within thiG at 2183.569 kb on - strand, within thiG at 2183.613 kb on + strand, within thiG at 2183.647 kb on + strand, within thiG at 2183.746 kb on + strand, within thiG at 2183.836 kb on + strand, within thiG at 2183.952 kb on + strand, within thiG at 2183.983 kb on + strand, within thiG at 2184.212 kb on + strand at 2184.252 kb on - strand at 2184.282 kb on + strand, within thiS at 2184.290 kb on - strand, within thiS at 2184.290 kb on - strand, within thiS at 2184.336 kb on + strand, within thiS at 2184.380 kb on + strand, within thiS at 2184.397 kb on + strand, within thiS at 2184.405 kb on - strand, within thiS at 2184.512 kb on - strand at 2184.543 kb on - strand at 2184.566 kb on + strand at 2184.594 kb on - strand at 2184.667 kb on + strand at 2184.673 kb on + strand at 2184.830 kb on - strand at 2184.847 kb on - strand at 2184.930 kb on - strand at 2185.019 kb on + strand, within DVU2096 at 2185.075 kb on - strand at 2185.182 kb on - strand at 2185.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine 5mM as nutrient remove 2,182,474 + thiH DVU2093 0.27 +0.3 2,182,853 + thiH DVU2093 0.55 -1.5 2,182,878 - thiH DVU2093 0.57 -2.5 2,182,887 + thiH DVU2093 0.58 -2.1 2,183,038 - thiH DVU2093 0.69 -0.4 2,183,038 - thiH DVU2093 0.69 -2.9 2,183,055 + thiH DVU2093 0.70 -2.8 2,183,063 + thiH DVU2093 0.71 -2.2 2,183,120 - thiH DVU2093 0.75 -1.8 2,183,125 - thiH DVU2093 0.76 +0.9 2,183,159 - thiH DVU2093 0.78 -2.0 2,183,193 - thiH DVU2093 0.81 -2.6 2,183,195 + thiH DVU2093 0.81 -2.6 2,183,220 - thiH DVU2093 0.83 -2.8 2,183,222 + thiH DVU2093 0.83 -2.2 2,183,229 + thiH DVU2093 0.84 -1.3 2,183,229 + thiH DVU2093 0.84 -1.9 2,183,237 - thiH DVU2093 0.84 -1.9 2,183,284 + thiH DVU2093 0.88 -1.9 2,183,344 - -2.7 2,183,430 + -2.9 2,183,502 + -2.4 2,183,524 - -1.3 2,183,525 - -2.7 2,183,563 + thiG DVU2094 0.14 -2.5 2,183,569 - thiG DVU2094 0.15 -1.2 2,183,613 + thiG DVU2094 0.20 -1.1 2,183,647 + thiG DVU2094 0.25 -3.2 2,183,746 + thiG DVU2094 0.37 -1.1 2,183,836 + thiG DVU2094 0.48 -1.6 2,183,952 + thiG DVU2094 0.63 -1.8 2,183,983 + thiG DVU2094 0.67 -2.3 2,184,212 + -0.8 2,184,252 - -4.5 2,184,282 + thiS DVU2095 0.17 -2.6 2,184,290 - thiS DVU2095 0.21 -1.3 2,184,290 - thiS DVU2095 0.21 -2.0 2,184,336 + thiS DVU2095 0.44 -1.6 2,184,380 + thiS DVU2095 0.66 -2.7 2,184,397 + thiS DVU2095 0.74 -2.5 2,184,405 - thiS DVU2095 0.78 -2.5 2,184,512 - +0.1 2,184,543 - -0.0 2,184,566 + -1.2 2,184,594 - -2.5 2,184,667 + -0.6 2,184,673 + +0.2 2,184,830 - -0.1 2,184,847 - -0.8 2,184,930 - -0.1 2,185,019 + DVU2096 0.48 +0.4 2,185,075 - -0.3 2,185,182 - -0.2 2,185,190 - -1.3
Or see this region's nucleotide sequence