Experiment: MoLS4 with L-Cysteine 5mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroA and DVU0464 are separated by 5 nucleotides DVU0464 and trpE are separated by 131 nucleotides
DVU0463: aroA - 3-phosphoshikimate 1-carboxyvinyltransferase (TIGR), at 530,629 to 531,948
aroA
DVU0464: DVU0464 - prephenate and/or arogenate dehydrogenase <small>(from data)</small>, at 531,954 to 532,721
DVU0464
DVU0465: trpE - anthranilate synthase, component I (TIGR), at 532,853 to 534,451
trpE
Position (kb) 531
532
533 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 531.925 kb on + strand at 531.986 kb on + strand at 532.039 kb on + strand, within DVU0464 at 532.087 kb on + strand, within DVU0464 at 532.260 kb on - strand, within DVU0464 at 532.347 kb on + strand, within DVU0464 at 532.436 kb on - strand, within DVU0464 at 532.436 kb on - strand, within DVU0464 at 532.552 kb on + strand, within DVU0464 at 532.557 kb on + strand, within DVU0464 at 532.678 kb on + strand at 532.692 kb on + strand at 532.700 kb on - strand at 532.722 kb on - strand at 532.737 kb on + strand at 532.844 kb on + strand at 532.917 kb on - strand at 532.938 kb on + strand at 532.948 kb on + strand at 532.951 kb on - strand at 532.956 kb on - strand at 532.961 kb on - strand at 533.078 kb on - strand, within trpE at 533.129 kb on + strand, within trpE at 533.153 kb on + strand, within trpE at 533.220 kb on + strand, within trpE at 533.286 kb on - strand, within trpE at 533.374 kb on - strand, within trpE at 533.461 kb on + strand, within trpE at 533.466 kb on + strand, within trpE at 533.489 kb on - strand, within trpE at 533.512 kb on + strand, within trpE at 533.547 kb on - strand, within trpE at 533.554 kb on - strand, within trpE at 533.608 kb on - strand, within trpE at 533.613 kb on - strand, within trpE at 533.615 kb on + strand, within trpE at 533.615 kb on + strand, within trpE at 533.623 kb on - strand, within trpE at 533.651 kb on - strand, within trpE at 533.687 kb on - strand, within trpE at 533.715 kb on - strand, within trpE at 533.719 kb on + strand, within trpE
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine 5mM as nutrient remove 531,925 + -1.4 531,986 + -0.0 532,039 + DVU0464 0.11 -1.1 532,087 + DVU0464 0.17 -1.3 532,260 - DVU0464 0.40 -1.4 532,347 + DVU0464 0.51 -2.4 532,436 - DVU0464 0.63 +3.3 532,436 - DVU0464 0.63 -0.9 532,552 + DVU0464 0.78 -1.6 532,557 + DVU0464 0.79 +0.0 532,678 + +0.1 532,692 + -1.1 532,700 - -0.5 532,722 - -1.3 532,737 + -1.1 532,844 + -0.9 532,917 - -1.1 532,938 + -0.5 532,948 + +0.9 532,951 - -2.3 532,956 - -1.3 532,961 - -1.7 533,078 - trpE DVU0465 0.14 -1.3 533,129 + trpE DVU0465 0.17 +1.1 533,153 + trpE DVU0465 0.19 -0.3 533,220 + trpE DVU0465 0.23 -0.4 533,286 - trpE DVU0465 0.27 -2.1 533,374 - trpE DVU0465 0.33 -1.4 533,461 + trpE DVU0465 0.38 -1.5 533,466 + trpE DVU0465 0.38 -2.0 533,489 - trpE DVU0465 0.40 -0.7 533,512 + trpE DVU0465 0.41 -1.1 533,547 - trpE DVU0465 0.43 -0.7 533,554 - trpE DVU0465 0.44 -1.6 533,608 - trpE DVU0465 0.47 -1.2 533,613 - trpE DVU0465 0.48 -1.6 533,615 + trpE DVU0465 0.48 -1.3 533,615 + trpE DVU0465 0.48 -1.4 533,623 - trpE DVU0465 0.48 -0.2 533,651 - trpE DVU0465 0.50 -1.3 533,687 - trpE DVU0465 0.52 -1.6 533,715 - trpE DVU0465 0.54 -2.4 533,719 + trpE DVU0465 0.54 -1.6
Or see this region's nucleotide sequence