Experiment: MoLS4 with L-Cysteine 5mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speA and lys1 are separated by 248 nucleotides lys1 and nspC are separated by 277 nucleotides
DVU0417: speA - arginine decarboxylase (TIGR), at 468,657 to 470,636
speA
DVU0418: lys1 - saccharopine dehydrogenase (TIGR), at 470,885 to 472,075
lys1
DVU0419: nspC - carboxynorspermidine decarboxylase (TIGR), at 472,353 to 473,525
nspC
Position (kb) 470
471
472
473 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 469.908 kb on - strand, within speA at 469.910 kb on - strand, within speA at 469.983 kb on - strand, within speA at 470.014 kb on - strand, within speA at 470.056 kb on - strand, within speA at 470.121 kb on - strand, within speA at 470.258 kb on + strand, within speA at 470.360 kb on - strand, within speA at 470.373 kb on + strand, within speA at 470.490 kb on - strand at 470.506 kb on + strand at 470.586 kb on + strand at 470.614 kb on + strand at 470.618 kb on + strand at 470.618 kb on + strand at 470.624 kb on + strand at 470.732 kb on - strand at 470.732 kb on - strand at 470.844 kb on + strand at 470.856 kb on + strand at 470.950 kb on + strand at 471.046 kb on + strand, within lys1 at 471.046 kb on + strand, within lys1 at 471.056 kb on + strand, within lys1 at 471.129 kb on + strand, within lys1 at 471.129 kb on + strand, within lys1 at 471.130 kb on - strand, within lys1 at 471.157 kb on - strand, within lys1 at 471.176 kb on - strand, within lys1 at 471.187 kb on + strand, within lys1 at 471.216 kb on - strand, within lys1 at 471.234 kb on - strand, within lys1 at 471.504 kb on - strand, within lys1 at 471.584 kb on + strand, within lys1 at 471.657 kb on + strand, within lys1 at 471.666 kb on + strand, within lys1 at 471.666 kb on + strand, within lys1 at 471.666 kb on + strand, within lys1 at 471.674 kb on - strand, within lys1 at 471.710 kb on + strand, within lys1 at 471.740 kb on + strand, within lys1 at 471.816 kb on + strand, within lys1 at 471.852 kb on - strand, within lys1 at 471.932 kb on - strand, within lys1 at 471.945 kb on - strand, within lys1 at 471.980 kb on + strand at 472.004 kb on - strand at 472.033 kb on + strand at 472.051 kb on + strand at 472.142 kb on - strand at 472.152 kb on - strand at 472.160 kb on - strand at 472.167 kb on + strand at 472.175 kb on - strand at 472.292 kb on + strand at 472.292 kb on + strand at 472.292 kb on + strand at 472.300 kb on - strand at 472.300 kb on - strand at 472.335 kb on + strand at 472.402 kb on - strand at 472.432 kb on - strand at 472.439 kb on - strand at 472.461 kb on + strand at 472.461 kb on + strand at 472.461 kb on + strand at 472.469 kb on - strand at 472.537 kb on + strand, within nspC at 472.537 kb on + strand, within nspC at 472.538 kb on - strand, within nspC at 472.562 kb on - strand, within nspC at 472.562 kb on - strand, within nspC at 472.603 kb on - strand, within nspC at 472.638 kb on + strand, within nspC at 472.712 kb on + strand, within nspC at 472.791 kb on + strand, within nspC at 472.897 kb on + strand, within nspC at 472.905 kb on - strand, within nspC at 472.959 kb on + strand, within nspC at 473.043 kb on - strand, within nspC at 473.056 kb on - strand, within nspC at 473.064 kb on - strand, within nspC
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine 5mM as nutrient remove 469,908 - speA DVU0417 0.63 -0.7 469,910 - speA DVU0417 0.63 -1.2 469,983 - speA DVU0417 0.67 -0.9 470,014 - speA DVU0417 0.69 +0.3 470,056 - speA DVU0417 0.71 -2.6 470,121 - speA DVU0417 0.74 -0.4 470,258 + speA DVU0417 0.81 +0.3 470,360 - speA DVU0417 0.86 -0.1 470,373 + speA DVU0417 0.87 -0.1 470,490 - -1.3 470,506 + -0.0 470,586 + -1.8 470,614 + -1.4 470,618 + -1.2 470,618 + -0.7 470,624 + -1.2 470,732 - -0.2 470,732 - -1.0 470,844 + -0.4 470,856 + +0.5 470,950 + -1.6 471,046 + lys1 DVU0418 0.14 -2.0 471,046 + lys1 DVU0418 0.14 -1.0 471,056 + lys1 DVU0418 0.14 -2.7 471,129 + lys1 DVU0418 0.20 -0.5 471,129 + lys1 DVU0418 0.20 -1.8 471,130 - lys1 DVU0418 0.21 +0.7 471,157 - lys1 DVU0418 0.23 -2.0 471,176 - lys1 DVU0418 0.24 +0.3 471,187 + lys1 DVU0418 0.25 +0.7 471,216 - lys1 DVU0418 0.28 -0.2 471,234 - lys1 DVU0418 0.29 -2.0 471,504 - lys1 DVU0418 0.52 -2.1 471,584 + lys1 DVU0418 0.59 -1.7 471,657 + lys1 DVU0418 0.65 -0.6 471,666 + lys1 DVU0418 0.66 -1.7 471,666 + lys1 DVU0418 0.66 -1.7 471,666 + lys1 DVU0418 0.66 -2.0 471,674 - lys1 DVU0418 0.66 -1.7 471,710 + lys1 DVU0418 0.69 +0.1 471,740 + lys1 DVU0418 0.72 -1.2 471,816 + lys1 DVU0418 0.78 -0.2 471,852 - lys1 DVU0418 0.81 -2.0 471,932 - lys1 DVU0418 0.88 -1.9 471,945 - lys1 DVU0418 0.89 -1.7 471,980 + -1.8 472,004 - -0.9 472,033 + -1.0 472,051 + +0.7 472,142 - +0.0 472,152 - -2.2 472,160 - -1.0 472,167 + -2.8 472,175 - +0.6 472,292 + -0.0 472,292 + +0.2 472,292 + +0.3 472,300 - +0.5 472,300 - -0.1 472,335 + -1.2 472,402 - -0.7 472,432 - -0.7 472,439 - -1.3 472,461 + -2.0 472,461 + -1.8 472,461 + -1.1 472,469 - -1.3 472,537 + nspC DVU0419 0.16 -0.7 472,537 + nspC DVU0419 0.16 -1.2 472,538 - nspC DVU0419 0.16 -1.8 472,562 - nspC DVU0419 0.18 -0.5 472,562 - nspC DVU0419 0.18 -2.8 472,603 - nspC DVU0419 0.21 -0.3 472,638 + nspC DVU0419 0.24 +1.3 472,712 + nspC DVU0419 0.31 -1.2 472,791 + nspC DVU0419 0.37 +0.7 472,897 + nspC DVU0419 0.46 -1.2 472,905 - nspC DVU0419 0.47 +0.5 472,959 + nspC DVU0419 0.52 -1.3 473,043 - nspC DVU0419 0.59 -0.3 473,056 - nspC DVU0419 0.60 -0.9 473,064 - nspC DVU0419 0.61 -1.7
Or see this region's nucleotide sequence