Experiment: MoLS4 with L-Cysteine 5mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rplT and rpmI are separated by 82 nucleotides rpmI and infC are separated by 169 nucleotides infC and thrS are separated by 7 nucleotides thrS and tRNA-Val are separated by 85 nucleotides tRNA-Val and DVU2539 are separated by 24 nucleotides DVU2539 and DVU2540 are separated by 5 nucleotides
DVU2535: rplT - ribosomal protein L20 (TIGR), at 2,647,904 to 2,648,257
rplT
DVU2536: rpmI - ribosomal protein L35 (TIGR), at 2,648,340 to 2,648,537
rpmI
DVU2537: infC - translation initiation factor IF-3 (TIGR), at 2,648,707 to 2,649,237
infC
DVU2538: thrS - threonyl-tRNA synthetase (TIGR), at 2,649,245 to 2,651,179
thrS
DVU_tRNA-Val-2: tRNA-Val - tRNA with anticodon GAC for Val (VIMSS-AUTO), at 2,651,265 to 2,651,339
tRNA-Val
DVU2539: DVU2539 - hypothetical protein (TIGR), at 2,651,364 to 2,651,486
DVU2539
DVU2540: DVU2540 - lyase, putative (TIGR), at 2,651,492 to 2,652,634
DVU2540
Position (kb)
2649
2650
2651
2652 Strain fitness (log2 ratio)
-2
-1
0
1 at 2648.574 kb on + strand at 2651.462 kb on + strand, within DVU2539 at 2651.479 kb on + strand at 2651.529 kb on + strand at 2651.551 kb on + strand at 2651.559 kb on - strand at 2651.595 kb on - strand at 2651.611 kb on + strand, within DVU2540 at 2651.617 kb on - strand, within DVU2540 at 2651.634 kb on - strand, within DVU2540 at 2651.634 kb on - strand, within DVU2540 at 2651.720 kb on - strand, within DVU2540 at 2651.732 kb on - strand, within DVU2540 at 2651.742 kb on + strand, within DVU2540 at 2651.778 kb on + strand, within DVU2540 at 2651.811 kb on - strand, within DVU2540 at 2651.829 kb on - strand, within DVU2540 at 2651.843 kb on + strand, within DVU2540 at 2651.870 kb on - strand, within DVU2540 at 2651.920 kb on - strand, within DVU2540 at 2651.946 kb on - strand, within DVU2540 at 2651.948 kb on - strand, within DVU2540 at 2651.957 kb on + strand, within DVU2540 at 2652.009 kb on - strand, within DVU2540 at 2652.091 kb on - strand, within DVU2540 at 2652.139 kb on - strand, within DVU2540 at 2652.165 kb on - strand, within DVU2540
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine 5mM as nutrient remove 2,648,574 + -0.1 2,651,462 + DVU2539 0.80 -0.2 2,651,479 + -0.4 2,651,529 + -0.3 2,651,551 + -0.7 2,651,559 - -0.2 2,651,595 - +1.2 2,651,611 + DVU2540 0.10 -0.5 2,651,617 - DVU2540 0.11 -0.9 2,651,634 - DVU2540 0.12 -0.6 2,651,634 - DVU2540 0.12 +0.8 2,651,720 - DVU2540 0.20 +0.0 2,651,732 - DVU2540 0.21 +0.8 2,651,742 + DVU2540 0.22 -0.5 2,651,778 + DVU2540 0.25 +1.5 2,651,811 - DVU2540 0.28 -1.9 2,651,829 - DVU2540 0.29 -0.4 2,651,843 + DVU2540 0.31 +0.8 2,651,870 - DVU2540 0.33 -0.0 2,651,920 - DVU2540 0.37 +0.1 2,651,946 - DVU2540 0.40 +0.3 2,651,948 - DVU2540 0.40 +0.2 2,651,957 + DVU2540 0.41 +1.6 2,652,009 - DVU2540 0.45 +0.5 2,652,091 - DVU2540 0.52 -0.2 2,652,139 - DVU2540 0.57 +1.0 2,652,165 - DVU2540 0.59 -0.5
Or see this region's nucleotide sequence