Experiment: MoLS4 with L-Cysteine 5mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tpiA and rimI are separated by 19 nucleotides rimI and idi are separated by 22 nucleotides idi and suhB overlap by 43 nucleotides suhB and mreB-2 are separated by 18 nucleotides
DVU1677: tpiA - triosephosphate isomerase (TIGR), at 1,756,281 to 1,757,036
tpiA
DVU1678: rimI - ribosomal-protein-alanine acetyltransferase (TIGR), at 1,757,056 to 1,757,550
rimI
DVU1679: idi - isopentenyl-diphosphate delta-isomerase (TIGR), at 1,757,573 to 1,758,109
idi
DVU1680: suhB - inositol-1-monophosphatase (TIGR), at 1,758,067 to 1,758,858
suhB
DVU1681: mreB-2 - rod shape-determining protein MreB (TIGR), at 1,758,877 to 1,759,899
mreB-2
Position (kb)
1757
1758
1759 Strain fitness (log2 ratio)
-2
-1
0
1 at 1757.061 kb on - strand at 1757.161 kb on + strand, within rimI at 1757.216 kb on - strand, within rimI at 1757.280 kb on + strand, within rimI at 1757.303 kb on - strand, within rimI at 1757.349 kb on + strand, within rimI at 1757.352 kb on - strand, within rimI at 1757.385 kb on + strand, within rimI at 1757.398 kb on + strand, within rimI at 1757.417 kb on - strand, within rimI at 1757.430 kb on + strand, within rimI at 1757.447 kb on + strand, within rimI at 1757.463 kb on + strand, within rimI at 1757.609 kb on + strand at 1757.609 kb on + strand at 1757.614 kb on + strand at 1757.924 kb on + strand, within idi at 1758.029 kb on - strand, within idi at 1758.056 kb on + strand at 1758.148 kb on + strand, within suhB at 1758.156 kb on - strand, within suhB at 1758.156 kb on - strand, within suhB at 1758.156 kb on - strand, within suhB at 1758.236 kb on - strand, within suhB at 1758.252 kb on - strand, within suhB at 1758.354 kb on - strand, within suhB at 1758.414 kb on + strand, within suhB at 1758.422 kb on - strand, within suhB at 1758.476 kb on + strand, within suhB at 1758.484 kb on + strand, within suhB at 1758.492 kb on - strand, within suhB at 1758.638 kb on - strand, within suhB at 1758.703 kb on - strand, within suhB at 1758.829 kb on + strand at 1758.920 kb on - strand at 1758.979 kb on - strand at 1758.979 kb on - strand at 1759.010 kb on + strand, within mreB-2 at 1759.079 kb on - strand, within mreB-2 at 1759.086 kb on + strand, within mreB-2
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine 5mM as nutrient remove 1,757,061 - -1.3 1,757,161 + rimI DVU1678 0.21 -0.2 1,757,216 - rimI DVU1678 0.32 -0.4 1,757,280 + rimI DVU1678 0.45 +1.0 1,757,303 - rimI DVU1678 0.50 -0.4 1,757,349 + rimI DVU1678 0.59 +0.6 1,757,352 - rimI DVU1678 0.60 -0.4 1,757,385 + rimI DVU1678 0.66 +0.2 1,757,398 + rimI DVU1678 0.69 -0.3 1,757,417 - rimI DVU1678 0.73 -1.1 1,757,430 + rimI DVU1678 0.76 -0.6 1,757,447 + rimI DVU1678 0.79 -0.8 1,757,463 + rimI DVU1678 0.82 -0.2 1,757,609 + +0.6 1,757,609 + +0.9 1,757,614 + -0.3 1,757,924 + idi DVU1679 0.65 +0.1 1,758,029 - idi DVU1679 0.85 +0.4 1,758,056 + +0.0 1,758,148 + suhB DVU1680 0.10 -0.9 1,758,156 - suhB DVU1680 0.11 -0.9 1,758,156 - suhB DVU1680 0.11 +0.5 1,758,156 - suhB DVU1680 0.11 -0.5 1,758,236 - suhB DVU1680 0.21 -0.9 1,758,252 - suhB DVU1680 0.23 -0.1 1,758,354 - suhB DVU1680 0.36 -2.2 1,758,414 + suhB DVU1680 0.44 +0.2 1,758,422 - suhB DVU1680 0.45 +1.2 1,758,476 + suhB DVU1680 0.52 +0.9 1,758,484 + suhB DVU1680 0.53 +0.6 1,758,492 - suhB DVU1680 0.54 -0.3 1,758,638 - suhB DVU1680 0.72 -0.9 1,758,703 - suhB DVU1680 0.80 +0.4 1,758,829 + +0.2 1,758,920 - -0.2 1,758,979 - -0.3 1,758,979 - -0.8 1,759,010 + mreB-2 DVU1681 0.13 +0.1 1,759,079 - mreB-2 DVU1681 0.20 +0.3 1,759,086 + mreB-2 DVU1681 0.20 -1.2
Or see this region's nucleotide sequence