Experiment: MoLS4 with L-Cysteine 5mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysA-1 and DVU1648 are separated by 73 nucleotides DVU1648 and mutS overlap by 17 nucleotides mutS and DVU1650 are separated by 163 nucleotides
DVU1647: lysA-1 - diaminopimelate decarboxylase (TIGR), at 1,726,001 to 1,727,239
lysA-1
DVU1648: DVU1648 - lipoprotein, putative (TIGR), at 1,727,313 to 1,727,753
DVU1648
DVU1649: mutS - DNA mismatch repair protein MutS (TIGR), at 1,727,737 to 1,730,454
mutS
DVU1650: DVU1650 - conserved hypothetical protein (TIGR), at 1,730,618 to 1,731,742
DVU1650
Position (kb)
1727
1728
1729
1730
1731 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1726.757 kb on + strand, within lysA-1 at 1726.774 kb on + strand, within lysA-1 at 1726.785 kb on + strand, within lysA-1 at 1726.810 kb on - strand, within lysA-1 at 1726.845 kb on - strand, within lysA-1 at 1726.934 kb on + strand, within lysA-1 at 1727.003 kb on + strand, within lysA-1 at 1727.022 kb on - strand, within lysA-1 at 1727.162 kb on + strand at 1727.227 kb on + strand at 1727.259 kb on + strand at 1727.297 kb on + strand at 1727.376 kb on + strand, within DVU1648 at 1727.390 kb on + strand, within DVU1648 at 1727.457 kb on - strand, within DVU1648 at 1727.558 kb on - strand, within DVU1648 at 1727.644 kb on - strand, within DVU1648 at 1727.705 kb on - strand, within DVU1648 at 1727.756 kb on - strand at 1727.848 kb on - strand at 1727.857 kb on + strand at 1727.865 kb on - strand at 1727.869 kb on + strand at 1727.891 kb on + strand at 1727.937 kb on - strand at 1728.075 kb on - strand, within mutS at 1728.082 kb on + strand, within mutS at 1728.153 kb on - strand, within mutS at 1728.161 kb on - strand, within mutS at 1728.174 kb on - strand, within mutS at 1728.272 kb on - strand, within mutS at 1728.370 kb on + strand, within mutS at 1728.602 kb on + strand, within mutS at 1728.655 kb on + strand, within mutS at 1728.663 kb on - strand, within mutS at 1728.864 kb on + strand, within mutS at 1728.999 kb on - strand, within mutS at 1729.119 kb on - strand, within mutS at 1729.220 kb on + strand, within mutS at 1729.220 kb on + strand, within mutS at 1729.220 kb on + strand, within mutS at 1729.228 kb on - strand, within mutS at 1729.236 kb on + strand, within mutS at 1729.245 kb on + strand, within mutS at 1729.261 kb on - strand, within mutS at 1729.268 kb on - strand, within mutS at 1729.352 kb on + strand, within mutS at 1729.397 kb on - strand, within mutS at 1729.404 kb on - strand, within mutS at 1729.404 kb on - strand, within mutS at 1729.420 kb on + strand, within mutS at 1729.420 kb on + strand, within mutS at 1729.428 kb on - strand, within mutS at 1729.670 kb on + strand, within mutS at 1729.670 kb on + strand, within mutS at 1729.670 kb on + strand, within mutS at 1729.680 kb on - strand, within mutS at 1729.764 kb on + strand, within mutS at 1730.071 kb on - strand, within mutS at 1730.203 kb on - strand at 1730.203 kb on - strand at 1730.387 kb on + strand at 1730.387 kb on + strand at 1730.390 kb on - strand at 1730.395 kb on - strand at 1730.514 kb on - strand at 1730.616 kb on + strand at 1730.624 kb on - strand at 1730.636 kb on + strand at 1730.776 kb on - strand, within DVU1650 at 1730.823 kb on - strand, within DVU1650 at 1730.843 kb on + strand, within DVU1650 at 1731.070 kb on + strand, within DVU1650 at 1731.082 kb on + strand, within DVU1650 at 1731.095 kb on - strand, within DVU1650 at 1731.174 kb on + strand, within DVU1650 at 1731.349 kb on + strand, within DVU1650 at 1731.370 kb on + strand, within DVU1650 at 1731.451 kb on + strand, within DVU1650
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine 5mM as nutrient remove 1,726,757 + lysA-1 DVU1647 0.61 +0.9 1,726,774 + lysA-1 DVU1647 0.62 -0.3 1,726,785 + lysA-1 DVU1647 0.63 -0.6 1,726,810 - lysA-1 DVU1647 0.65 -0.3 1,726,845 - lysA-1 DVU1647 0.68 -0.3 1,726,934 + lysA-1 DVU1647 0.75 +0.5 1,727,003 + lysA-1 DVU1647 0.81 +0.1 1,727,022 - lysA-1 DVU1647 0.82 -0.3 1,727,162 + -0.4 1,727,227 + -2.1 1,727,259 + -0.5 1,727,297 + +0.5 1,727,376 + DVU1648 0.14 +1.4 1,727,390 + DVU1648 0.17 +0.7 1,727,457 - DVU1648 0.33 -0.7 1,727,558 - DVU1648 0.56 -0.8 1,727,644 - DVU1648 0.75 -0.6 1,727,705 - DVU1648 0.89 -0.4 1,727,756 - -0.7 1,727,848 - -0.7 1,727,857 + +1.1 1,727,865 - +0.9 1,727,869 + +0.1 1,727,891 + -2.6 1,727,937 - -0.4 1,728,075 - mutS DVU1649 0.12 +0.0 1,728,082 + mutS DVU1649 0.13 -0.5 1,728,153 - mutS DVU1649 0.15 -0.6 1,728,161 - mutS DVU1649 0.16 +0.2 1,728,174 - mutS DVU1649 0.16 +1.0 1,728,272 - mutS DVU1649 0.20 +0.4 1,728,370 + mutS DVU1649 0.23 -0.7 1,728,602 + mutS DVU1649 0.32 -0.6 1,728,655 + mutS DVU1649 0.34 -0.3 1,728,663 - mutS DVU1649 0.34 -0.3 1,728,864 + mutS DVU1649 0.41 -1.8 1,728,999 - mutS DVU1649 0.46 -0.9 1,729,119 - mutS DVU1649 0.51 +0.0 1,729,220 + mutS DVU1649 0.55 +1.1 1,729,220 + mutS DVU1649 0.55 -0.3 1,729,220 + mutS DVU1649 0.55 -2.3 1,729,228 - mutS DVU1649 0.55 +0.7 1,729,236 + mutS DVU1649 0.55 -0.4 1,729,245 + mutS DVU1649 0.55 -0.1 1,729,261 - mutS DVU1649 0.56 -0.8 1,729,268 - mutS DVU1649 0.56 +0.4 1,729,352 + mutS DVU1649 0.59 +1.4 1,729,397 - mutS DVU1649 0.61 +0.4 1,729,404 - mutS DVU1649 0.61 -0.3 1,729,404 - mutS DVU1649 0.61 -0.3 1,729,420 + mutS DVU1649 0.62 -0.3 1,729,420 + mutS DVU1649 0.62 -0.4 1,729,428 - mutS DVU1649 0.62 +0.1 1,729,670 + mutS DVU1649 0.71 -0.7 1,729,670 + mutS DVU1649 0.71 +0.1 1,729,670 + mutS DVU1649 0.71 -0.2 1,729,680 - mutS DVU1649 0.71 -0.4 1,729,764 + mutS DVU1649 0.75 -0.6 1,730,071 - mutS DVU1649 0.86 -0.2 1,730,203 - -0.9 1,730,203 - -0.7 1,730,387 + +0.4 1,730,387 + -0.1 1,730,390 - +0.2 1,730,395 - -0.6 1,730,514 - -0.5 1,730,616 + -0.6 1,730,624 - -1.2 1,730,636 + +0.8 1,730,776 - DVU1650 0.14 +0.5 1,730,823 - DVU1650 0.18 -1.0 1,730,843 + DVU1650 0.20 -0.1 1,731,070 + DVU1650 0.40 -0.1 1,731,082 + DVU1650 0.41 +2.5 1,731,095 - DVU1650 0.42 +1.5 1,731,174 + DVU1650 0.49 -1.0 1,731,349 + DVU1650 0.65 -0.6 1,731,370 + DVU1650 0.67 +0.9 1,731,451 + DVU1650 0.74 +0.9
Or see this region's nucleotide sequence