Experiment: MoLS4 with L-Cysteine 5mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1598 and crcB are separated by 44 nucleotides crcB and DVU1600 are separated by 38 nucleotides DVU1600 and DVU1601 are separated by 90 nucleotides DVU1601 and clpA overlap by 1 nucleotides
DVU1598: DVU1598 - conserved hypothetical protein (TIGR), at 1,681,361 to 1,681,705
DVU1598
DVU1599: crcB - crcB protein (TIGR), at 1,681,750 to 1,682,124
crcB
DVU1600: DVU1600 - adenylate cyclase (TIGR), at 1,682,163 to 1,682,774
DVU1600
DVU1601: DVU1601 - ATP-dependent Clp protease adaptor protein ClpS (TIGR), at 1,682,865 to 1,683,179
DVU1601
DVU1602: clpA - ATP-dependent Clp protease, ATP-binding subunit ClpA (TIGR), at 1,683,179 to 1,685,509
clpA
Position (kb)
1682
1683 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1681.252 kb on + strand at 1681.265 kb on - strand at 1681.323 kb on + strand at 1681.353 kb on - strand at 1681.366 kb on + strand at 1681.386 kb on + strand at 1681.394 kb on - strand at 1681.434 kb on + strand, within DVU1598 at 1681.465 kb on + strand, within DVU1598 at 1681.473 kb on - strand, within DVU1598 at 1681.495 kb on + strand, within DVU1598 at 1681.620 kb on + strand, within DVU1598 at 1681.620 kb on + strand, within DVU1598 at 1681.630 kb on + strand, within DVU1598 at 1681.675 kb on + strand at 1681.688 kb on + strand at 1681.819 kb on + strand, within crcB at 1681.827 kb on - strand, within crcB at 1681.866 kb on + strand, within crcB at 1681.967 kb on + strand, within crcB at 1682.008 kb on - strand, within crcB at 1682.018 kb on - strand, within crcB at 1682.131 kb on + strand at 1682.198 kb on + strand at 1682.245 kb on + strand, within DVU1600 at 1682.313 kb on + strand, within DVU1600 at 1682.344 kb on - strand, within DVU1600 at 1682.347 kb on + strand, within DVU1600 at 1682.347 kb on + strand, within DVU1600 at 1682.358 kb on - strand, within DVU1600 at 1682.360 kb on + strand, within DVU1600 at 1682.375 kb on + strand, within DVU1600 at 1682.507 kb on + strand, within DVU1600 at 1682.507 kb on + strand, within DVU1600 at 1682.539 kb on + strand, within DVU1600 at 1682.541 kb on - strand, within DVU1600 at 1682.644 kb on + strand, within DVU1600 at 1682.644 kb on + strand, within DVU1600 at 1682.652 kb on - strand, within DVU1600 at 1682.728 kb on + strand at 1682.754 kb on + strand at 1682.830 kb on - strand at 1682.947 kb on - strand, within DVU1601 at 1682.974 kb on - strand, within DVU1601 at 1682.981 kb on - strand, within DVU1601 at 1682.981 kb on - strand, within DVU1601 at 1683.058 kb on + strand, within DVU1601 at 1683.081 kb on + strand, within DVU1601 at 1683.088 kb on + strand, within DVU1601 at 1683.112 kb on - strand, within DVU1601 at 1683.112 kb on - strand, within DVU1601 at 1683.158 kb on - strand at 1683.201 kb on - strand at 1683.293 kb on - strand at 1683.308 kb on + strand at 1683.379 kb on - strand at 1683.410 kb on + strand at 1683.425 kb on - strand, within clpA at 1683.526 kb on + strand, within clpA at 1683.528 kb on - strand, within clpA at 1683.534 kb on - strand, within clpA at 1683.653 kb on + strand, within clpA at 1683.687 kb on + strand, within clpA at 1683.710 kb on + strand, within clpA at 1683.731 kb on + strand, within clpA at 1683.772 kb on - strand, within clpA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine 5mM as nutrient remove 1,681,252 + -0.2 1,681,265 - +0.2 1,681,323 + -0.1 1,681,353 - -0.3 1,681,366 + -0.4 1,681,386 + +1.5 1,681,394 - +0.1 1,681,434 + DVU1598 0.21 -0.5 1,681,465 + DVU1598 0.30 -1.1 1,681,473 - DVU1598 0.32 -0.2 1,681,495 + DVU1598 0.39 +0.2 1,681,620 + DVU1598 0.75 -1.2 1,681,620 + DVU1598 0.75 -0.0 1,681,630 + DVU1598 0.78 +0.6 1,681,675 + -0.1 1,681,688 + -0.3 1,681,819 + crcB DVU1599 0.18 +0.9 1,681,827 - crcB DVU1599 0.21 +0.0 1,681,866 + crcB DVU1599 0.31 -0.2 1,681,967 + crcB DVU1599 0.58 -0.6 1,682,008 - crcB DVU1599 0.69 -0.3 1,682,018 - crcB DVU1599 0.71 -0.1 1,682,131 + -0.9 1,682,198 + +1.5 1,682,245 + DVU1600 0.13 -0.7 1,682,313 + DVU1600 0.25 +2.1 1,682,344 - DVU1600 0.30 -0.9 1,682,347 + DVU1600 0.30 +1.4 1,682,347 + DVU1600 0.30 +1.6 1,682,358 - DVU1600 0.32 +1.6 1,682,360 + DVU1600 0.32 +1.2 1,682,375 + DVU1600 0.35 +1.3 1,682,507 + DVU1600 0.56 +1.8 1,682,507 + DVU1600 0.56 +2.1 1,682,539 + DVU1600 0.61 +0.9 1,682,541 - DVU1600 0.62 -0.2 1,682,644 + DVU1600 0.79 +2.0 1,682,644 + DVU1600 0.79 +2.0 1,682,652 - DVU1600 0.80 +0.9 1,682,728 + +1.5 1,682,754 + +0.2 1,682,830 - -0.6 1,682,947 - DVU1601 0.26 -0.0 1,682,974 - DVU1601 0.35 +0.7 1,682,981 - DVU1601 0.37 -0.0 1,682,981 - DVU1601 0.37 -0.4 1,683,058 + DVU1601 0.61 -0.9 1,683,081 + DVU1601 0.69 -0.6 1,683,088 + DVU1601 0.71 +0.7 1,683,112 - DVU1601 0.78 +1.9 1,683,112 - DVU1601 0.78 +0.0 1,683,158 - -0.9 1,683,201 - -0.8 1,683,293 - -0.3 1,683,308 + -0.6 1,683,379 - +1.2 1,683,410 + -0.3 1,683,425 - clpA DVU1602 0.11 -0.2 1,683,526 + clpA DVU1602 0.15 +0.1 1,683,528 - clpA DVU1602 0.15 +0.0 1,683,534 - clpA DVU1602 0.15 -0.2 1,683,653 + clpA DVU1602 0.20 -0.6 1,683,687 + clpA DVU1602 0.22 -0.8 1,683,710 + clpA DVU1602 0.23 -4.0 1,683,731 + clpA DVU1602 0.24 -0.3 1,683,772 - clpA DVU1602 0.25 +0.7
Or see this region's nucleotide sequence