Experiment: MoLS4 with L-Cysteine 5mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nfo and DVU1225 are separated by 486 nucleotides DVU1225 and DVU1226 are separated by 11 nucleotides DVU1226 and tpX are separated by 215 nucleotides tpX and DVU1230 are separated by 195 nucleotides
DVU1224: nfo - endonuclease IV (TIGR), at 1,315,670 to 1,316,518
nfo
DVU1225: DVU1225 - hypothetical protein (TIGR), at 1,317,005 to 1,317,457
DVU1225
DVU1226: DVU1226 - hypothetical protein (TIGR), at 1,317,469 to 1,317,564
DVU1226
DVU1228: tpX - thiol peroxidase (TIGR), at 1,317,780 to 1,318,298
tpX
DVU1230: DVU1230 - hypothetical protein (TIGR), at 1,318,494 to 1,318,604
DVU1230
Position (kb)
1317
1318 Strain fitness (log2 ratio)
-2
-1
0
1 at 1316.478 kb on + strand at 1316.530 kb on - strand at 1316.551 kb on - strand at 1316.685 kb on + strand at 1316.697 kb on - strand at 1316.773 kb on + strand at 1316.773 kb on + strand at 1316.781 kb on - strand at 1316.781 kb on - strand at 1316.809 kb on - strand at 1316.866 kb on - strand at 1316.913 kb on + strand at 1316.932 kb on + strand at 1316.940 kb on - strand at 1316.998 kb on - strand at 1317.021 kb on + strand at 1317.116 kb on + strand, within DVU1225 at 1317.162 kb on + strand, within DVU1225 at 1317.175 kb on - strand, within DVU1225 at 1317.221 kb on - strand, within DVU1225 at 1317.291 kb on - strand, within DVU1225 at 1317.452 kb on - strand at 1317.479 kb on + strand, within DVU1226 at 1317.656 kb on - strand at 1317.699 kb on - strand at 1317.841 kb on + strand, within tpX at 1317.927 kb on + strand, within tpX at 1317.936 kb on - strand, within tpX at 1317.953 kb on + strand, within tpX at 1317.957 kb on - strand, within tpX at 1317.960 kb on + strand, within tpX at 1318.055 kb on - strand, within tpX at 1318.057 kb on + strand, within tpX at 1318.123 kb on - strand, within tpX at 1318.169 kb on - strand, within tpX at 1318.221 kb on + strand, within tpX at 1318.236 kb on - strand, within tpX at 1318.255 kb on - strand at 1318.309 kb on - strand at 1318.309 kb on - strand at 1318.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine 5mM as nutrient remove 1,316,478 + -0.0 1,316,530 - +1.0 1,316,551 - -0.3 1,316,685 + -1.6 1,316,697 - -0.2 1,316,773 + +0.1 1,316,773 + +0.3 1,316,781 - +0.1 1,316,781 - +1.7 1,316,809 - -0.2 1,316,866 - -0.6 1,316,913 + -0.2 1,316,932 + -0.2 1,316,940 - -0.3 1,316,998 - -0.7 1,317,021 + -0.2 1,317,116 + DVU1225 0.25 +1.2 1,317,162 + DVU1225 0.35 -0.5 1,317,175 - DVU1225 0.38 +1.1 1,317,221 - DVU1225 0.48 -0.3 1,317,291 - DVU1225 0.63 -0.9 1,317,452 - -0.1 1,317,479 + DVU1226 0.10 +0.3 1,317,656 - +0.1 1,317,699 - -0.8 1,317,841 + tpX DVU1228 0.12 -0.1 1,317,927 + tpX DVU1228 0.28 +0.6 1,317,936 - tpX DVU1228 0.30 +0.5 1,317,953 + tpX DVU1228 0.33 -0.7 1,317,957 - tpX DVU1228 0.34 -0.8 1,317,960 + tpX DVU1228 0.35 -1.2 1,318,055 - tpX DVU1228 0.53 +0.3 1,318,057 + tpX DVU1228 0.53 -0.0 1,318,123 - tpX DVU1228 0.66 -1.2 1,318,169 - tpX DVU1228 0.75 -0.9 1,318,221 + tpX DVU1228 0.85 -1.9 1,318,236 - tpX DVU1228 0.88 -0.4 1,318,255 - -1.0 1,318,309 - -0.3 1,318,309 - -0.6 1,318,358 - -1.7
Or see this region's nucleotide sequence