Experiment: MOLS with alpha-ketoglutaric 2.5mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpD and trpC overlap by 8 nucleotides trpC and trpF-1 are separated by 47 nucleotides trpF-1 and trpB-2 overlap by 4 nucleotides
DVU0467: trpD - anthranilate phosphoribosyltransferase (TIGR), at 535,058 to 536,056
trpD
DVU0468: trpC - indole-3-glycerol phosphate synthase (TIGR), at 536,049 to 536,822
trpC
DVU0469: trpF-1 - N-(5-phosphoribosyl)anthranilate isomerase (TIGR), at 536,870 to 537,724
trpF-1
DVU0470: trpB-2 - tryptophan synthase, beta subunit (TIGR), at 537,721 to 538,908
trpB-2
Position (kb) 536
537
538 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 535.871 kb on - strand, within trpD at 535.897 kb on + strand, within trpD at 535.905 kb on - strand, within trpD at 535.920 kb on - strand, within trpD at 535.938 kb on + strand, within trpD at 535.942 kb on - strand, within trpD at 535.942 kb on - strand, within trpD at 535.955 kb on + strand, within trpD at 535.955 kb on + strand, within trpD at 535.963 kb on - strand at 535.983 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand at 536.095 kb on - strand at 536.158 kb on + strand, within trpC at 536.164 kb on - strand, within trpC at 536.166 kb on - strand, within trpC at 536.166 kb on - strand, within trpC at 536.179 kb on + strand, within trpC at 536.179 kb on + strand, within trpC at 536.179 kb on + strand, within trpC at 536.301 kb on - strand, within trpC at 536.303 kb on + strand, within trpC at 536.311 kb on - strand, within trpC at 536.318 kb on + strand, within trpC at 536.318 kb on + strand, within trpC at 536.369 kb on - strand, within trpC at 536.425 kb on + strand, within trpC at 536.461 kb on + strand, within trpC at 536.467 kb on + strand, within trpC at 536.506 kb on - strand, within trpC at 536.511 kb on + strand, within trpC at 536.519 kb on - strand, within trpC at 536.519 kb on - strand, within trpC at 536.547 kb on - strand, within trpC at 536.566 kb on + strand, within trpC at 536.611 kb on + strand, within trpC at 536.619 kb on - strand, within trpC at 536.669 kb on + strand, within trpC at 536.750 kb on - strand at 536.757 kb on + strand at 536.757 kb on + strand at 536.765 kb on - strand at 536.799 kb on + strand at 536.807 kb on - strand at 536.860 kb on + strand at 536.979 kb on + strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.135 kb on + strand, within trpF-1 at 537.206 kb on - strand, within trpF-1 at 537.288 kb on + strand, within trpF-1 at 537.389 kb on - strand, within trpF-1 at 537.476 kb on + strand, within trpF-1 at 537.479 kb on - strand, within trpF-1 at 537.489 kb on - strand, within trpF-1 at 537.574 kb on - strand, within trpF-1 at 537.579 kb on - strand, within trpF-1 at 537.664 kb on - strand at 537.671 kb on - strand at 537.688 kb on - strand at 537.690 kb on + strand at 537.692 kb on + strand at 538.023 kb on - strand, within trpB-2 at 538.233 kb on + strand, within trpB-2 at 538.236 kb on - strand, within trpB-2 at 538.244 kb on - strand, within trpB-2 at 538.254 kb on + strand, within trpB-2 at 538.264 kb on + strand, within trpB-2 at 538.432 kb on + strand, within trpB-2 at 538.527 kb on - strand, within trpB-2 at 538.529 kb on - strand, within trpB-2 at 538.594 kb on - strand, within trpB-2
Per-strain Table
Position Strand Gene LocusTag Fraction MOLS with alpha-ketoglutaric 2.5mM as nutrient remove 535,871 - trpD DVU0467 0.81 -4.0 535,897 + trpD DVU0467 0.84 -0.6 535,905 - trpD DVU0467 0.85 -3.3 535,920 - trpD DVU0467 0.86 -4.4 535,938 + trpD DVU0467 0.88 -1.2 535,942 - trpD DVU0467 0.88 -1.1 535,942 - trpD DVU0467 0.88 +0.2 535,955 + trpD DVU0467 0.90 -2.0 535,955 + trpD DVU0467 0.90 -3.5 535,963 - -3.6 535,983 + -1.5 535,985 + -4.2 535,985 + +0.9 535,985 + -2.5 536,095 - -5.7 536,158 + trpC DVU0468 0.14 -4.0 536,164 - trpC DVU0468 0.15 -2.2 536,166 - trpC DVU0468 0.15 -2.7 536,166 - trpC DVU0468 0.15 -3.3 536,179 + trpC DVU0468 0.17 -5.3 536,179 + trpC DVU0468 0.17 -6.5 536,179 + trpC DVU0468 0.17 -3.4 536,301 - trpC DVU0468 0.33 -2.7 536,303 + trpC DVU0468 0.33 -0.9 536,311 - trpC DVU0468 0.34 -4.6 536,318 + trpC DVU0468 0.35 -5.3 536,318 + trpC DVU0468 0.35 -1.1 536,369 - trpC DVU0468 0.41 -3.7 536,425 + trpC DVU0468 0.49 -5.0 536,461 + trpC DVU0468 0.53 -3.7 536,467 + trpC DVU0468 0.54 -0.3 536,506 - trpC DVU0468 0.59 -2.4 536,511 + trpC DVU0468 0.60 -3.7 536,519 - trpC DVU0468 0.61 -2.2 536,519 - trpC DVU0468 0.61 -3.7 536,547 - trpC DVU0468 0.64 +0.2 536,566 + trpC DVU0468 0.67 -1.9 536,611 + trpC DVU0468 0.73 -4.5 536,619 - trpC DVU0468 0.74 -5.0 536,669 + trpC DVU0468 0.80 -0.9 536,750 - -2.2 536,757 + -1.0 536,757 + +0.5 536,765 - -3.6 536,799 + -0.3 536,807 - -3.0 536,860 + -2.4 536,979 + trpF-1 DVU0469 0.13 -4.6 537,037 - trpF-1 DVU0469 0.20 -1.0 537,037 - trpF-1 DVU0469 0.20 -3.0 537,044 - trpF-1 DVU0469 0.20 -3.0 537,044 - trpF-1 DVU0469 0.20 -5.7 537,135 + trpF-1 DVU0469 0.31 -4.3 537,206 - trpF-1 DVU0469 0.39 -0.3 537,288 + trpF-1 DVU0469 0.49 -4.2 537,389 - trpF-1 DVU0469 0.61 -3.7 537,476 + trpF-1 DVU0469 0.71 -0.6 537,479 - trpF-1 DVU0469 0.71 -4.2 537,489 - trpF-1 DVU0469 0.72 -0.3 537,574 - trpF-1 DVU0469 0.82 -3.9 537,579 - trpF-1 DVU0469 0.83 -0.5 537,664 - +0.4 537,671 - -0.9 537,688 - +0.0 537,690 + +1.5 537,692 + -1.6 538,023 - trpB-2 DVU0470 0.25 -1.1 538,233 + trpB-2 DVU0470 0.43 -2.6 538,236 - trpB-2 DVU0470 0.43 -0.3 538,244 - trpB-2 DVU0470 0.44 -2.8 538,254 + trpB-2 DVU0470 0.45 -1.7 538,264 + trpB-2 DVU0470 0.46 -2.8 538,432 + trpB-2 DVU0470 0.60 -0.8 538,527 - trpB-2 DVU0470 0.68 -0.8 538,529 - trpB-2 DVU0470 0.68 -4.4 538,594 - trpB-2 DVU0470 0.73 -4.6
Or see this region's nucleotide sequence