Experiment: MoLS4 with L-Valine (HPLC) and L-Leucine 10mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcrA and thiL overlap by 4 nucleotides thiL and DVU0158 are separated by 20 nucleotides DVU0158 and DVU0159 are separated by 213 nucleotides DVU0159 and DVU0160 are separated by 25 nucleotides
DVU0156: pcrA - ATP-dependent DNA helicase, UvrD/REP family (TIGR), at 192,240 to 194,399
pcrA
DVU0157: thiL - thiamin-monophosphate kinase (TIGR), at 194,396 to 195,367
thiL
DVU0158: DVU0158 - conserved hypothetical protein TIGR00104 (TIGR), at 195,388 to 196,386
DVU0158
DVU0159: DVU0159 - thioesterase family protein (TIGR), at 196,600 to 197,121
DVU0159
DVU0160: DVU0160 - carbohydrate isomerase, KpsF/GutQ family (TIGR), at 197,147 to 198,142
DVU0160
Position (kb) 195
196
197 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 195.379 kb on + strand at 195.416 kb on - strand at 195.427 kb on + strand at 195.435 kb on - strand at 195.535 kb on + strand, within DVU0158 at 195.540 kb on + strand, within DVU0158 at 195.541 kb on + strand, within DVU0158 at 195.592 kb on - strand, within DVU0158 at 195.756 kb on - strand, within DVU0158 at 195.766 kb on - strand, within DVU0158 at 195.774 kb on + strand, within DVU0158 at 195.787 kb on - strand, within DVU0158 at 195.821 kb on + strand, within DVU0158 at 195.858 kb on + strand, within DVU0158 at 195.858 kb on + strand, within DVU0158 at 195.881 kb on - strand, within DVU0158 at 195.969 kb on + strand, within DVU0158 at 196.031 kb on - strand, within DVU0158 at 196.031 kb on - strand, within DVU0158 at 196.060 kb on - strand, within DVU0158 at 196.127 kb on - strand, within DVU0158 at 196.131 kb on + strand, within DVU0158 at 196.139 kb on - strand, within DVU0158 at 196.146 kb on + strand, within DVU0158 at 196.189 kb on - strand, within DVU0158 at 196.207 kb on - strand, within DVU0158 at 196.207 kb on - strand, within DVU0158 at 196.304 kb on + strand at 196.312 kb on - strand at 196.372 kb on - strand at 196.543 kb on + strand at 196.551 kb on - strand at 196.572 kb on - strand at 196.572 kb on - strand at 196.625 kb on + strand at 196.706 kb on - strand, within DVU0159 at 196.706 kb on - strand, within DVU0159 at 196.795 kb on + strand, within DVU0159 at 196.825 kb on + strand, within DVU0159 at 196.968 kb on + strand, within DVU0159 at 196.968 kb on - strand, within DVU0159 at 196.978 kb on - strand, within DVU0159 at 197.026 kb on + strand, within DVU0159 at 197.116 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Valine (HPLC) and L-Leucine 10mM as nutrient remove 195,379 + -1.1 195,416 - -0.1 195,427 + -0.6 195,435 - +0.6 195,535 + DVU0158 0.15 -0.9 195,540 + DVU0158 0.15 -0.2 195,541 + DVU0158 0.15 -1.5 195,592 - DVU0158 0.20 -1.2 195,756 - DVU0158 0.37 -0.2 195,766 - DVU0158 0.38 -1.5 195,774 + DVU0158 0.39 +0.1 195,787 - DVU0158 0.40 +0.2 195,821 + DVU0158 0.43 -1.2 195,858 + DVU0158 0.47 -0.5 195,858 + DVU0158 0.47 -1.9 195,881 - DVU0158 0.49 +1.8 195,969 + DVU0158 0.58 +0.8 196,031 - DVU0158 0.64 -0.5 196,031 - DVU0158 0.64 +0.9 196,060 - DVU0158 0.67 +3.0 196,127 - DVU0158 0.74 -1.2 196,131 + DVU0158 0.74 -1.6 196,139 - DVU0158 0.75 -0.9 196,146 + DVU0158 0.76 -2.7 196,189 - DVU0158 0.80 +0.3 196,207 - DVU0158 0.82 -1.2 196,207 - DVU0158 0.82 -0.3 196,304 + -1.1 196,312 - -2.0 196,372 - -2.1 196,543 + -0.1 196,551 - -3.0 196,572 - +0.2 196,572 - -2.2 196,625 + +0.8 196,706 - DVU0159 0.20 -1.3 196,706 - DVU0159 0.20 -0.1 196,795 + DVU0159 0.37 -0.2 196,825 + DVU0159 0.43 +1.4 196,968 + DVU0159 0.70 -1.0 196,968 - DVU0159 0.70 -0.0 196,978 - DVU0159 0.72 -0.4 197,026 + DVU0159 0.82 -2.9 197,116 - -0.5
Or see this region's nucleotide sequence