Experiment: MoLS4 with L-Valine (HPLC) and L-Leucine 10mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3139 and tRNA-Val are separated by 269 nucleotides tRNA-Val and DVU3140 are separated by 69 nucleotides DVU3140 and DVU3141 are separated by 286 nucleotides DVU3141 and DVU3142 are separated by 223 nucleotides
DVU3139: DVU3139 - bacterial extracellular solute-binding protein, family 3 (TIGR), at 3,286,423 to 3,287,199
DVU3139
DVU_tRNA-Val-3: tRNA-Val - tRNA with anticodon TAC for Val (VIMSS-AUTO), at 3,287,469 to 3,287,544
tRNA-Val
DVU3140: DVU3140 - capsular polysaccharide transport protein, putative (TIGR), at 3,287,614 to 3,289,227
DVU3140
DVU3141: DVU3141 - lipoprotein, putative (TIGR), at 3,289,514 to 3,289,894
DVU3141
DVU3142: DVU3142 - sigma-54 dependent transcriptional regulator (TIGR), at 3,290,118 to 3,291,821
DVU3142
Position (kb)
3287
3288
3289
3290 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3286.846 kb on + strand, within DVU3139 at 3287.037 kb on - strand, within DVU3139 at 3287.289 kb on + strand at 3287.329 kb on + strand at 3287.397 kb on + strand at 3287.405 kb on - strand at 3287.406 kb on - strand at 3287.587 kb on + strand at 3287.597 kb on + strand at 3287.607 kb on - strand at 3287.648 kb on + strand at 3287.761 kb on - strand at 3287.815 kb on - strand, within DVU3140 at 3287.859 kb on - strand, within DVU3140 at 3287.895 kb on - strand, within DVU3140 at 3287.983 kb on + strand, within DVU3140 at 3288.061 kb on + strand, within DVU3140 at 3288.103 kb on + strand, within DVU3140 at 3288.116 kb on - strand, within DVU3140 at 3288.116 kb on - strand, within DVU3140 at 3288.116 kb on - strand, within DVU3140 at 3288.145 kb on - strand, within DVU3140 at 3288.444 kb on + strand, within DVU3140 at 3288.444 kb on + strand, within DVU3140 at 3288.469 kb on + strand, within DVU3140 at 3288.477 kb on - strand, within DVU3140 at 3288.572 kb on - strand, within DVU3140 at 3288.795 kb on + strand, within DVU3140 at 3288.795 kb on + strand, within DVU3140 at 3288.821 kb on - strand, within DVU3140 at 3288.831 kb on - strand, within DVU3140 at 3288.841 kb on - strand, within DVU3140 at 3288.888 kb on + strand, within DVU3140 at 3288.896 kb on - strand, within DVU3140 at 3288.953 kb on - strand, within DVU3140 at 3288.955 kb on + strand, within DVU3140 at 3288.957 kb on + strand, within DVU3140 at 3288.965 kb on - strand, within DVU3140 at 3288.980 kb on + strand, within DVU3140 at 3288.980 kb on + strand, within DVU3140 at 3288.988 kb on - strand, within DVU3140 at 3288.988 kb on - strand, within DVU3140 at 3288.993 kb on + strand, within DVU3140 at 3288.993 kb on + strand, within DVU3140 at 3289.001 kb on - strand, within DVU3140 at 3289.034 kb on + strand, within DVU3140 at 3289.129 kb on + strand at 3289.160 kb on + strand at 3289.168 kb on - strand at 3289.170 kb on + strand at 3289.170 kb on + strand at 3289.177 kb on + strand at 3289.183 kb on + strand at 3289.191 kb on - strand at 3289.212 kb on - strand at 3289.308 kb on - strand at 3289.375 kb on + strand at 3289.401 kb on + strand at 3289.409 kb on - strand at 3289.412 kb on + strand at 3289.449 kb on + strand at 3289.456 kb on + strand at 3289.480 kb on - strand at 3289.480 kb on - strand at 3289.519 kb on + strand at 3289.521 kb on + strand at 3289.521 kb on + strand at 3289.591 kb on + strand, within DVU3141 at 3289.634 kb on + strand, within DVU3141 at 3289.634 kb on + strand, within DVU3141 at 3289.634 kb on + strand, within DVU3141 at 3289.877 kb on + strand at 3289.880 kb on - strand at 3289.937 kb on + strand at 3289.938 kb on + strand at 3289.938 kb on + strand at 3290.003 kb on + strand at 3290.017 kb on + strand at 3290.020 kb on + strand at 3290.023 kb on - strand at 3290.025 kb on - strand at 3290.044 kb on + strand at 3290.109 kb on + strand at 3290.117 kb on - strand at 3290.136 kb on + strand at 3290.149 kb on - strand at 3290.174 kb on - strand at 3290.196 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Valine (HPLC) and L-Leucine 10mM as nutrient remove 3,286,846 + DVU3139 0.54 -0.9 3,287,037 - DVU3139 0.79 +0.3 3,287,289 + +0.0 3,287,329 + +0.7 3,287,397 + -0.2 3,287,405 - -0.6 3,287,406 - -1.0 3,287,587 + -0.3 3,287,597 + -0.2 3,287,607 - +1.3 3,287,648 + -2.0 3,287,761 - +0.2 3,287,815 - DVU3140 0.12 -1.8 3,287,859 - DVU3140 0.15 -0.1 3,287,895 - DVU3140 0.17 -0.2 3,287,983 + DVU3140 0.23 -0.6 3,288,061 + DVU3140 0.28 -1.9 3,288,103 + DVU3140 0.30 +4.3 3,288,116 - DVU3140 0.31 -0.6 3,288,116 - DVU3140 0.31 -0.3 3,288,116 - DVU3140 0.31 -0.1 3,288,145 - DVU3140 0.33 -1.5 3,288,444 + DVU3140 0.51 -3.0 3,288,444 + DVU3140 0.51 +0.0 3,288,469 + DVU3140 0.53 -0.3 3,288,477 - DVU3140 0.53 -1.1 3,288,572 - DVU3140 0.59 +1.9 3,288,795 + DVU3140 0.73 -0.3 3,288,795 + DVU3140 0.73 +0.7 3,288,821 - DVU3140 0.75 +0.2 3,288,831 - DVU3140 0.75 +0.1 3,288,841 - DVU3140 0.76 -2.0 3,288,888 + DVU3140 0.79 -0.7 3,288,896 - DVU3140 0.79 -0.8 3,288,953 - DVU3140 0.83 -0.1 3,288,955 + DVU3140 0.83 -0.4 3,288,957 + DVU3140 0.83 -3.0 3,288,965 - DVU3140 0.84 -0.5 3,288,980 + DVU3140 0.85 -1.9 3,288,980 + DVU3140 0.85 -0.9 3,288,988 - DVU3140 0.85 +0.3 3,288,988 - DVU3140 0.85 -1.8 3,288,993 + DVU3140 0.85 -0.4 3,288,993 + DVU3140 0.85 -0.6 3,289,001 - DVU3140 0.86 -0.1 3,289,034 + DVU3140 0.88 -0.8 3,289,129 + +0.3 3,289,160 + -0.3 3,289,168 - +0.7 3,289,170 + +1.3 3,289,170 + +0.2 3,289,177 + -0.4 3,289,183 + +0.2 3,289,191 - -2.0 3,289,212 - -0.9 3,289,308 - -0.7 3,289,375 + +0.6 3,289,401 + -1.6 3,289,409 - +0.3 3,289,412 + -1.9 3,289,449 + -0.7 3,289,456 + -0.1 3,289,480 - -2.0 3,289,480 - -1.4 3,289,519 + -0.6 3,289,521 + +0.4 3,289,521 + -0.9 3,289,591 + DVU3141 0.20 +2.3 3,289,634 + DVU3141 0.31 -2.2 3,289,634 + DVU3141 0.31 -1.4 3,289,634 + DVU3141 0.31 -0.3 3,289,877 + +0.3 3,289,880 - -0.2 3,289,937 + -1.6 3,289,938 + -0.2 3,289,938 + -0.7 3,290,003 + -2.2 3,290,017 + -0.3 3,290,020 + -1.9 3,290,023 - -0.8 3,290,025 - -2.1 3,290,044 + -0.3 3,290,109 + +0.4 3,290,117 - -0.3 3,290,136 + -1.4 3,290,149 - -0.4 3,290,174 - -1.3 3,290,196 - +1.2
Or see this region's nucleotide sequence