Experiment: MoLS4 with L-Valine (HPLC) and L-Leucine 10mM as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1092 and DVU1093 are separated by 189 nucleotides DVU1093 and argH are separated by 132 nucleotides argH and argG are separated by 3 nucleotides
DVU1092: DVU1092 - sodium-dependent symporter family protein (TIGR), at 1,197,183 to 1,198,544
DVU1092
DVU1093: DVU1093 - HAD-superfamily hydrolase, subfamily IA, variant 3 (TIGR), at 1,198,734 to 1,199,264
DVU1093
DVU1094: argH - argininosuccinate lyase (TIGR), at 1,199,397 to 1,200,779
argH
DVU1095: argG - argininosuccinate synthase (TIGR), at 1,200,783 to 1,201,973
argG
Position (kb)
1199
1200
1201 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 1198.430 kb on - strand at 1198.468 kb on - strand at 1198.503 kb on - strand at 1198.538 kb on + strand at 1198.663 kb on + strand at 1198.730 kb on - strand at 1198.786 kb on - strand at 1198.848 kb on - strand, within DVU1093 at 1198.927 kb on - strand, within DVU1093 at 1199.081 kb on - strand, within DVU1093 at 1199.211 kb on + strand, within DVU1093 at 1199.226 kb on - strand at 1199.294 kb on - strand at 1199.328 kb on - strand at 1199.572 kb on + strand, within argH at 1199.611 kb on + strand, within argH at 1199.621 kb on + strand, within argH at 1199.716 kb on + strand, within argH at 1199.804 kb on - strand, within argH at 1199.820 kb on + strand, within argH at 1199.855 kb on + strand, within argH at 1199.867 kb on + strand, within argH at 1199.880 kb on - strand, within argH at 1200.010 kb on - strand, within argH at 1200.055 kb on + strand, within argH at 1200.302 kb on + strand, within argH at 1200.336 kb on + strand, within argH at 1200.419 kb on + strand, within argH at 1200.427 kb on - strand, within argH at 1200.728 kb on + strand at 1200.733 kb on + strand at 1200.733 kb on + strand at 1200.733 kb on + strand at 1200.741 kb on - strand at 1200.742 kb on + strand at 1200.835 kb on + strand at 1200.857 kb on - strand at 1200.874 kb on - strand at 1200.881 kb on + strand at 1200.936 kb on + strand, within argG at 1201.069 kb on - strand, within argG at 1201.152 kb on + strand, within argG at 1201.251 kb on - strand, within argG at 1201.269 kb on - strand, within argG at 1201.291 kb on + strand, within argG at 1201.325 kb on - strand, within argG at 1201.325 kb on - strand, within argG at 1201.339 kb on - strand, within argG at 1201.371 kb on - strand, within argG at 1201.432 kb on + strand, within argG at 1201.452 kb on - strand, within argG at 1201.479 kb on + strand, within argG at 1201.498 kb on + strand, within argG at 1201.542 kb on - strand, within argG at 1201.562 kb on + strand, within argG at 1201.583 kb on + strand, within argG at 1201.593 kb on + strand, within argG at 1201.688 kb on - strand, within argG at 1201.691 kb on + strand, within argG
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Valine (HPLC) and L-Leucine 10mM as nutrient remove 1,198,430 - -0.7 1,198,468 - -0.8 1,198,503 - -0.9 1,198,538 + -0.6 1,198,663 + -1.0 1,198,730 - -1.7 1,198,786 - +1.3 1,198,848 - DVU1093 0.21 +0.2 1,198,927 - DVU1093 0.36 +0.2 1,199,081 - DVU1093 0.65 -1.2 1,199,211 + DVU1093 0.90 -1.7 1,199,226 - -1.3 1,199,294 - -1.9 1,199,328 - -1.4 1,199,572 + argH DVU1094 0.13 -5.2 1,199,611 + argH DVU1094 0.15 -4.4 1,199,621 + argH DVU1094 0.16 -1.9 1,199,716 + argH DVU1094 0.23 -4.4 1,199,804 - argH DVU1094 0.29 -7.0 1,199,820 + argH DVU1094 0.31 -0.2 1,199,855 + argH DVU1094 0.33 -2.7 1,199,867 + argH DVU1094 0.34 -4.6 1,199,880 - argH DVU1094 0.35 -2.0 1,200,010 - argH DVU1094 0.44 -0.2 1,200,055 + argH DVU1094 0.48 -3.7 1,200,302 + argH DVU1094 0.65 +2.4 1,200,336 + argH DVU1094 0.68 -5.3 1,200,419 + argH DVU1094 0.74 -2.2 1,200,427 - argH DVU1094 0.74 -3.0 1,200,728 + -5.6 1,200,733 + -0.4 1,200,733 + -0.8 1,200,733 + -4.7 1,200,741 - -1.8 1,200,742 + -5.3 1,200,835 + -2.0 1,200,857 - -1.4 1,200,874 - -4.0 1,200,881 + -0.4 1,200,936 + argG DVU1095 0.13 -2.8 1,201,069 - argG DVU1095 0.24 -6.1 1,201,152 + argG DVU1095 0.31 -6.0 1,201,251 - argG DVU1095 0.39 -1.6 1,201,269 - argG DVU1095 0.41 +0.1 1,201,291 + argG DVU1095 0.43 -4.6 1,201,325 - argG DVU1095 0.46 -3.7 1,201,325 - argG DVU1095 0.46 -3.7 1,201,339 - argG DVU1095 0.47 -4.2 1,201,371 - argG DVU1095 0.49 -1.2 1,201,432 + argG DVU1095 0.54 -5.0 1,201,452 - argG DVU1095 0.56 -5.0 1,201,479 + argG DVU1095 0.58 -0.4 1,201,498 + argG DVU1095 0.60 +1.4 1,201,542 - argG DVU1095 0.64 +0.4 1,201,562 + argG DVU1095 0.65 -1.9 1,201,583 + argG DVU1095 0.67 -5.4 1,201,593 + argG DVU1095 0.68 -5.0 1,201,688 - argG DVU1095 0.76 -5.4 1,201,691 + argG DVU1095 0.76 -5.1
Or see this region's nucleotide sequence