Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4348 and BT4349 are separated by 25 nucleotides BT4349 and BT4350 are separated by 198 nucleotides BT4350 and BT4351 are separated by 104 nucleotides
BT4348: BT4348 - hypothetical protein (NCBI ptt file), at 5,730,667 to 5,731,053
BT4348
BT4349: BT4349 - hypothetical protein (NCBI ptt file), at 5,731,079 to 5,731,546
BT4349
BT4350: BT4350 - conserved hypothetical protein (NCBI ptt file), at 5,731,745 to 5,732,533
BT4350
BT4351: BT4351 - hypothetical protein (NCBI ptt file), at 5,732,638 to 5,733,705
BT4351
Position (kb)
5731
5732
5733 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5730.842 kb on - strand, within BT4348 at 5730.843 kb on + strand, within BT4348 at 5731.021 kb on + strand at 5731.021 kb on + strand at 5731.021 kb on + strand at 5731.056 kb on + strand at 5731.056 kb on + strand at 5731.120 kb on + strand at 5731.318 kb on + strand, within BT4349 at 5731.318 kb on + strand, within BT4349 at 5731.402 kb on + strand, within BT4349 at 5731.402 kb on + strand, within BT4349 at 5731.589 kb on - strand at 5731.602 kb on + strand at 5731.615 kb on + strand at 5731.674 kb on - strand at 5731.708 kb on - strand at 5731.711 kb on + strand at 5731.711 kb on + strand at 5731.711 kb on + strand at 5731.712 kb on - strand at 5731.747 kb on - strand at 5731.747 kb on - strand at 5731.801 kb on + strand at 5731.832 kb on - strand, within BT4350 at 5731.986 kb on - strand, within BT4350 at 5732.129 kb on + strand, within BT4350 at 5732.223 kb on - strand, within BT4350 at 5732.313 kb on + strand, within BT4350 at 5732.334 kb on - strand, within BT4350 at 5732.334 kb on - strand, within BT4350 at 5732.334 kb on - strand, within BT4350 at 5732.334 kb on - strand, within BT4350 at 5732.334 kb on - strand, within BT4350 at 5732.398 kb on - strand, within BT4350 at 5732.409 kb on - strand, within BT4350 at 5732.524 kb on + strand at 5732.524 kb on + strand at 5732.525 kb on - strand at 5732.525 kb on - strand at 5732.586 kb on + strand at 5732.586 kb on + strand at 5732.725 kb on + strand at 5732.753 kb on + strand, within BT4351 at 5732.754 kb on - strand, within BT4351 at 5732.754 kb on - strand, within BT4351 at 5732.804 kb on + strand, within BT4351 at 5732.804 kb on - strand, within BT4351 at 5732.830 kb on + strand, within BT4351 at 5732.831 kb on - strand, within BT4351 at 5732.905 kb on + strand, within BT4351 at 5732.905 kb on + strand, within BT4351 at 5732.905 kb on + strand, within BT4351 at 5732.950 kb on + strand, within BT4351 at 5732.964 kb on + strand, within BT4351 at 5732.982 kb on - strand, within BT4351 at 5733.038 kb on + strand, within BT4351 at 5733.038 kb on + strand, within BT4351 at 5733.051 kb on - strand, within BT4351 at 5733.296 kb on + strand, within BT4351 at 5733.340 kb on - strand, within BT4351 at 5733.348 kb on + strand, within BT4351 at 5733.355 kb on - strand, within BT4351 at 5733.428 kb on + strand, within BT4351
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 5,730,842 - BT4348 0.45 +1.1 5,730,843 + BT4348 0.45 -1.1 5,731,021 + -0.8 5,731,021 + -0.8 5,731,021 + -0.7 5,731,056 + -0.7 5,731,056 + -1.6 5,731,120 + -2.7 5,731,318 + BT4349 0.51 -2.6 5,731,318 + BT4349 0.51 -0.5 5,731,402 + BT4349 0.69 +0.8 5,731,402 + BT4349 0.69 -1.8 5,731,589 - +0.5 5,731,602 + -2.3 5,731,615 + -3.9 5,731,674 - -0.7 5,731,708 - +0.0 5,731,711 + +0.6 5,731,711 + +1.1 5,731,711 + +0.3 5,731,712 - +1.5 5,731,747 - -0.9 5,731,747 - -2.9 5,731,801 + -0.4 5,731,832 - BT4350 0.11 -1.9 5,731,986 - BT4350 0.31 -1.0 5,732,129 + BT4350 0.49 -2.3 5,732,223 - BT4350 0.61 -2.5 5,732,313 + BT4350 0.72 -1.1 5,732,334 - BT4350 0.75 -0.8 5,732,334 - BT4350 0.75 -0.8 5,732,334 - BT4350 0.75 -1.1 5,732,334 - BT4350 0.75 -0.1 5,732,334 - BT4350 0.75 -1.7 5,732,398 - BT4350 0.83 +0.0 5,732,409 - BT4350 0.84 -1.8 5,732,524 + -0.5 5,732,524 + -0.2 5,732,525 - -0.8 5,732,525 - -0.5 5,732,586 + +1.5 5,732,586 + -0.7 5,732,725 + -1.2 5,732,753 + BT4351 0.11 -1.8 5,732,754 - BT4351 0.11 +3.1 5,732,754 - BT4351 0.11 -1.1 5,732,804 + BT4351 0.16 -0.1 5,732,804 - BT4351 0.16 -2.7 5,732,830 + BT4351 0.18 -0.6 5,732,831 - BT4351 0.18 -0.8 5,732,905 + BT4351 0.25 -1.3 5,732,905 + BT4351 0.25 -3.1 5,732,905 + BT4351 0.25 +0.7 5,732,950 + BT4351 0.29 -0.2 5,732,964 + BT4351 0.31 +0.9 5,732,982 - BT4351 0.32 +0.5 5,733,038 + BT4351 0.37 +1.8 5,733,038 + BT4351 0.37 -0.1 5,733,051 - BT4351 0.39 -0.8 5,733,296 + BT4351 0.62 +0.8 5,733,340 - BT4351 0.66 -0.5 5,733,348 + BT4351 0.66 -1.8 5,733,355 - BT4351 0.67 -3.3 5,733,428 + BT4351 0.74 -0.9
Or see this region's nucleotide sequence