Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2953 and BT2954 are separated by 7 nucleotides BT2954 and BT2955 are separated by 28 nucleotides
BT2953: BT2953 - L-rhamnose-proton symporter (NCBI ptt file), at 3,720,092 to 3,721,099
BT2953
BT2954: BT2954 - conserved hypothetical protein (NCBI ptt file), at 3,721,107 to 3,721,532
BT2954
BT2955: BT2955 - formate acetyltransferase 2 (NCBI ptt file), at 3,721,561 to 3,723,705
BT2955
Position (kb)
3721
3722 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3720.128 kb on + strand at 3720.218 kb on - strand, within BT2953 at 3720.218 kb on - strand, within BT2953 at 3720.269 kb on - strand, within BT2953 at 3720.288 kb on + strand, within BT2953 at 3720.295 kb on + strand, within BT2953 at 3720.306 kb on + strand, within BT2953 at 3720.315 kb on - strand, within BT2953 at 3720.335 kb on + strand, within BT2953 at 3720.354 kb on - strand, within BT2953 at 3720.400 kb on + strand, within BT2953 at 3720.418 kb on + strand, within BT2953 at 3720.446 kb on + strand, within BT2953 at 3720.470 kb on - strand, within BT2953 at 3720.538 kb on + strand, within BT2953 at 3720.539 kb on - strand, within BT2953 at 3720.539 kb on - strand, within BT2953 at 3720.572 kb on + strand, within BT2953 at 3720.631 kb on + strand, within BT2953 at 3720.632 kb on - strand, within BT2953 at 3720.651 kb on - strand, within BT2953 at 3720.712 kb on + strand, within BT2953 at 3720.772 kb on + strand, within BT2953 at 3720.787 kb on + strand, within BT2953 at 3720.836 kb on + strand, within BT2953 at 3720.836 kb on + strand, within BT2953 at 3720.839 kb on + strand, within BT2953 at 3720.839 kb on + strand, within BT2953 at 3720.906 kb on + strand, within BT2953 at 3720.906 kb on + strand, within BT2953 at 3720.908 kb on + strand, within BT2953 at 3720.908 kb on + strand, within BT2953 at 3720.908 kb on + strand, within BT2953 at 3720.908 kb on + strand, within BT2953 at 3720.909 kb on - strand, within BT2953 at 3720.909 kb on - strand, within BT2953 at 3720.909 kb on - strand, within BT2953 at 3720.909 kb on - strand, within BT2953 at 3720.909 kb on - strand, within BT2953 at 3720.928 kb on + strand, within BT2953 at 3720.928 kb on + strand, within BT2953 at 3720.934 kb on + strand, within BT2953 at 3721.084 kb on + strand at 3721.085 kb on - strand at 3721.085 kb on - strand at 3721.099 kb on + strand at 3721.100 kb on - strand at 3721.100 kb on - strand at 3721.138 kb on - strand at 3721.218 kb on + strand, within BT2954 at 3721.227 kb on + strand, within BT2954 at 3721.281 kb on + strand, within BT2954 at 3721.282 kb on + strand, within BT2954 at 3721.282 kb on + strand, within BT2954 at 3721.283 kb on - strand, within BT2954 at 3721.283 kb on - strand, within BT2954 at 3721.301 kb on + strand, within BT2954 at 3721.305 kb on + strand, within BT2954 at 3721.316 kb on - strand, within BT2954 at 3721.374 kb on + strand, within BT2954 at 3721.380 kb on + strand, within BT2954 at 3721.380 kb on + strand, within BT2954 at 3721.381 kb on - strand, within BT2954 at 3721.381 kb on - strand, within BT2954 at 3721.453 kb on + strand, within BT2954 at 3721.458 kb on + strand, within BT2954 at 3721.459 kb on - strand, within BT2954 at 3721.555 kb on + strand at 3721.585 kb on + strand at 3721.585 kb on - strand at 3721.690 kb on + strand at 3721.691 kb on - strand at 3721.691 kb on - strand at 3721.691 kb on - strand at 3721.707 kb on + strand at 3721.707 kb on + strand at 3721.708 kb on - strand at 3721.719 kb on + strand at 3721.768 kb on + strand at 3721.768 kb on + strand at 3721.769 kb on - strand at 3721.769 kb on - strand at 3721.798 kb on - strand, within BT2955 at 3721.857 kb on + strand, within BT2955 at 3721.858 kb on - strand, within BT2955 at 3721.927 kb on - strand, within BT2955 at 3721.986 kb on + strand, within BT2955 at 3721.986 kb on + strand, within BT2955 at 3722.050 kb on + strand, within BT2955 at 3722.051 kb on - strand, within BT2955 at 3722.147 kb on - strand, within BT2955 at 3722.297 kb on + strand, within BT2955 at 3722.297 kb on + strand, within BT2955 at 3722.297 kb on + strand, within BT2955 at 3722.363 kb on - strand, within BT2955 at 3722.365 kb on + strand, within BT2955 at 3722.385 kb on - strand, within BT2955 at 3722.513 kb on + strand, within BT2955 at 3722.519 kb on - strand, within BT2955
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 3,720,128 + +0.8 3,720,218 - BT2953 0.12 -2.5 3,720,218 - BT2953 0.12 -1.7 3,720,269 - BT2953 0.18 -0.9 3,720,288 + BT2953 0.19 -1.1 3,720,295 + BT2953 0.20 -0.4 3,720,306 + BT2953 0.21 -0.4 3,720,315 - BT2953 0.22 -0.5 3,720,335 + BT2953 0.24 -0.8 3,720,354 - BT2953 0.26 -0.3 3,720,400 + BT2953 0.31 -0.9 3,720,418 + BT2953 0.32 -1.2 3,720,446 + BT2953 0.35 -0.1 3,720,470 - BT2953 0.38 -2.2 3,720,538 + BT2953 0.44 -0.7 3,720,539 - BT2953 0.44 -2.1 3,720,539 - BT2953 0.44 -2.3 3,720,572 + BT2953 0.48 -1.0 3,720,631 + BT2953 0.53 -2.2 3,720,632 - BT2953 0.54 -1.1 3,720,651 - BT2953 0.55 +0.0 3,720,712 + BT2953 0.62 -0.0 3,720,772 + BT2953 0.67 -0.6 3,720,787 + BT2953 0.69 -0.4 3,720,836 + BT2953 0.74 -0.9 3,720,836 + BT2953 0.74 -0.2 3,720,839 + BT2953 0.74 +0.1 3,720,839 + BT2953 0.74 -1.2 3,720,906 + BT2953 0.81 -1.9 3,720,906 + BT2953 0.81 -0.0 3,720,908 + BT2953 0.81 +0.7 3,720,908 + BT2953 0.81 +0.0 3,720,908 + BT2953 0.81 -1.0 3,720,908 + BT2953 0.81 -0.4 3,720,909 - BT2953 0.81 +2.0 3,720,909 - BT2953 0.81 +0.6 3,720,909 - BT2953 0.81 -2.4 3,720,909 - BT2953 0.81 -1.2 3,720,909 - BT2953 0.81 +0.1 3,720,928 + BT2953 0.83 -0.4 3,720,928 + BT2953 0.83 -0.5 3,720,934 + BT2953 0.84 -0.2 3,721,084 + -1.5 3,721,085 - +0.0 3,721,085 - +1.6 3,721,099 + -1.9 3,721,100 - +1.6 3,721,100 - -3.2 3,721,138 - +2.3 3,721,218 + BT2954 0.26 -1.5 3,721,227 + BT2954 0.28 +1.4 3,721,281 + BT2954 0.41 +1.6 3,721,282 + BT2954 0.41 -2.1 3,721,282 + BT2954 0.41 -2.4 3,721,283 - BT2954 0.41 -2.4 3,721,283 - BT2954 0.41 +0.1 3,721,301 + BT2954 0.46 +0.1 3,721,305 + BT2954 0.46 -3.2 3,721,316 - BT2954 0.49 -3.4 3,721,374 + BT2954 0.63 -1.9 3,721,380 + BT2954 0.64 -0.7 3,721,380 + BT2954 0.64 -1.8 3,721,381 - BT2954 0.64 +0.6 3,721,381 - BT2954 0.64 -1.8 3,721,453 + BT2954 0.81 -1.4 3,721,458 + BT2954 0.82 -0.4 3,721,459 - BT2954 0.83 -1.4 3,721,555 + -2.2 3,721,585 + -2.2 3,721,585 - +0.6 3,721,690 + -0.6 3,721,691 - -2.4 3,721,691 - -3.2 3,721,691 - -1.4 3,721,707 + -1.0 3,721,707 + -1.2 3,721,708 - -0.9 3,721,719 + -0.4 3,721,768 + -0.4 3,721,768 + -2.5 3,721,769 - -3.1 3,721,769 - -1.5 3,721,798 - BT2955 0.11 -1.8 3,721,857 + BT2955 0.14 -0.2 3,721,858 - BT2955 0.14 -1.5 3,721,927 - BT2955 0.17 -0.5 3,721,986 + BT2955 0.20 -2.3 3,721,986 + BT2955 0.20 -2.1 3,722,050 + BT2955 0.23 -1.4 3,722,051 - BT2955 0.23 -2.0 3,722,147 - BT2955 0.27 -1.2 3,722,297 + BT2955 0.34 -0.6 3,722,297 + BT2955 0.34 -0.7 3,722,297 + BT2955 0.34 +0.0 3,722,363 - BT2955 0.37 -2.3 3,722,365 + BT2955 0.37 +1.6 3,722,385 - BT2955 0.38 -0.8 3,722,513 + BT2955 0.44 -3.1 3,722,519 - BT2955 0.45 +0.6
Or see this region's nucleotide sequence