Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2745 and BT2746 overlap by 4 nucleotides BT2746 and BT2747 are separated by 272 nucleotides
BT2745: BT2745 - thiol:disulfide interchange protein (NCBI ptt file), at 3,400,447 to 3,401,661
BT2745
BT2746: BT2746 - thiol:disulfide interchange protein tlpA (NCBI ptt file), at 3,401,658 to 3,403,286
BT2746
BT2747: BT2747 - 3-deoxy-D-manno-octulosonic-acid transferase (NCBI ptt file), at 3,403,559 to 3,404,782
BT2747
Position (kb)
3401
3402
3403
3404 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3400.743 kb on - strand, within BT2745 at 3400.743 kb on - strand, within BT2745 at 3400.820 kb on + strand, within BT2745 at 3400.820 kb on + strand, within BT2745 at 3400.869 kb on + strand, within BT2745 at 3400.882 kb on - strand, within BT2745 at 3400.892 kb on + strand, within BT2745 at 3401.041 kb on - strand, within BT2745 at 3401.041 kb on - strand, within BT2745 at 3401.054 kb on + strand, within BT2745 at 3401.088 kb on + strand, within BT2745 at 3401.089 kb on - strand, within BT2745 at 3401.145 kb on + strand, within BT2745 at 3401.146 kb on - strand, within BT2745 at 3401.189 kb on - strand, within BT2745 at 3401.267 kb on + strand, within BT2745 at 3401.291 kb on + strand, within BT2745 at 3401.324 kb on + strand, within BT2745 at 3401.325 kb on - strand, within BT2745 at 3401.429 kb on + strand, within BT2745 at 3401.491 kb on + strand, within BT2745 at 3401.502 kb on - strand, within BT2745 at 3401.521 kb on - strand, within BT2745 at 3401.705 kb on - strand at 3401.707 kb on + strand at 3401.707 kb on + strand at 3401.719 kb on + strand at 3401.733 kb on + strand at 3401.752 kb on + strand at 3401.752 kb on + strand at 3401.753 kb on - strand at 3401.755 kb on + strand at 3401.767 kb on + strand at 3401.771 kb on + strand at 3401.771 kb on + strand at 3401.772 kb on - strand at 3401.772 kb on - strand at 3401.775 kb on + strand at 3401.776 kb on - strand at 3401.778 kb on - strand at 3401.816 kb on + strand at 3401.817 kb on - strand at 3401.817 kb on - strand at 3401.817 kb on - strand at 3401.819 kb on + strand at 3401.820 kb on - strand at 3401.895 kb on + strand, within BT2746 at 3401.912 kb on - strand, within BT2746 at 3401.912 kb on - strand, within BT2746 at 3401.950 kb on + strand, within BT2746 at 3401.969 kb on + strand at 3402.013 kb on + strand, within BT2746 at 3402.027 kb on + strand, within BT2746 at 3402.027 kb on + strand, within BT2746 at 3402.028 kb on - strand, within BT2746 at 3402.028 kb on - strand, within BT2746 at 3402.068 kb on + strand, within BT2746 at 3402.076 kb on + strand, within BT2746 at 3402.077 kb on - strand, within BT2746 at 3402.162 kb on + strand, within BT2746 at 3402.415 kb on - strand, within BT2746 at 3402.453 kb on + strand, within BT2746 at 3402.489 kb on + strand, within BT2746 at 3402.566 kb on + strand, within BT2746 at 3402.567 kb on - strand, within BT2746 at 3402.646 kb on + strand, within BT2746 at 3402.646 kb on + strand, within BT2746 at 3402.667 kb on - strand, within BT2746 at 3402.715 kb on + strand, within BT2746 at 3402.726 kb on + strand, within BT2746 at 3402.726 kb on + strand, within BT2746 at 3402.727 kb on - strand, within BT2746 at 3402.780 kb on - strand, within BT2746 at 3402.874 kb on - strand, within BT2746 at 3402.893 kb on + strand, within BT2746 at 3402.894 kb on - strand, within BT2746 at 3402.939 kb on - strand, within BT2746 at 3403.008 kb on + strand, within BT2746 at 3403.031 kb on - strand, within BT2746 at 3403.053 kb on + strand, within BT2746 at 3403.057 kb on + strand, within BT2746 at 3403.057 kb on + strand, within BT2746 at 3403.058 kb on - strand, within BT2746 at 3403.099 kb on + strand at 3403.100 kb on - strand, within BT2746 at 3403.150 kb on - strand at 3403.189 kb on - strand at 3403.198 kb on + strand at 3403.214 kb on + strand at 3403.217 kb on - strand at 3403.262 kb on - strand at 3403.277 kb on - strand at 3403.310 kb on + strand at 3403.317 kb on - strand at 3403.367 kb on + strand at 3403.368 kb on - strand at 3403.419 kb on - strand at 3403.430 kb on - strand at 3403.430 kb on - strand at 3403.438 kb on - strand at 3403.440 kb on - strand at 3403.440 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 3,400,743 - BT2745 0.24 -0.5 3,400,743 - BT2745 0.24 -0.3 3,400,820 + BT2745 0.31 -1.1 3,400,820 + BT2745 0.31 -0.3 3,400,869 + BT2745 0.35 -1.2 3,400,882 - BT2745 0.36 -0.7 3,400,892 + BT2745 0.37 -1.7 3,401,041 - BT2745 0.49 -0.3 3,401,041 - BT2745 0.49 -1.5 3,401,054 + BT2745 0.50 +1.7 3,401,088 + BT2745 0.53 -2.0 3,401,089 - BT2745 0.53 -2.9 3,401,145 + BT2745 0.57 +0.1 3,401,146 - BT2745 0.58 -1.7 3,401,189 - BT2745 0.61 -0.7 3,401,267 + BT2745 0.67 +1.1 3,401,291 + BT2745 0.69 -1.0 3,401,324 + BT2745 0.72 -1.2 3,401,325 - BT2745 0.72 -1.4 3,401,429 + BT2745 0.81 -0.9 3,401,491 + BT2745 0.86 -1.5 3,401,502 - BT2745 0.87 +1.7 3,401,521 - BT2745 0.88 +0.0 3,401,705 - -1.0 3,401,707 + -0.7 3,401,707 + -3.0 3,401,719 + -1.3 3,401,733 + -0.0 3,401,752 + -1.7 3,401,752 + +0.3 3,401,753 - -1.7 3,401,755 + -0.9 3,401,767 + +0.2 3,401,771 + -0.8 3,401,771 + -0.3 3,401,772 - -2.7 3,401,772 - -0.3 3,401,775 + -1.5 3,401,776 - -1.8 3,401,778 - -1.2 3,401,816 + -0.7 3,401,817 - -0.7 3,401,817 - +0.9 3,401,817 - -1.2 3,401,819 + -1.6 3,401,820 - +0.1 3,401,895 + BT2746 0.15 -2.3 3,401,912 - BT2746 0.16 +0.7 3,401,912 - BT2746 0.16 -1.8 3,401,950 + BT2746 0.18 +0.7 3,401,969 + -0.7 3,402,013 + BT2746 0.22 -4.7 3,402,027 + BT2746 0.23 -4.6 3,402,027 + BT2746 0.23 +0.1 3,402,028 - BT2746 0.23 -1.7 3,402,028 - BT2746 0.23 -1.3 3,402,068 + BT2746 0.25 -1.2 3,402,076 + BT2746 0.26 -0.1 3,402,077 - BT2746 0.26 +0.7 3,402,162 + BT2746 0.31 -2.9 3,402,415 - BT2746 0.46 -0.3 3,402,453 + BT2746 0.49 -2.2 3,402,489 + BT2746 0.51 -1.1 3,402,566 + BT2746 0.56 -1.9 3,402,567 - BT2746 0.56 -1.7 3,402,646 + BT2746 0.61 +0.1 3,402,646 + BT2746 0.61 -2.5 3,402,667 - BT2746 0.62 -0.9 3,402,715 + BT2746 0.65 -1.2 3,402,726 + BT2746 0.66 -1.9 3,402,726 + BT2746 0.66 +0.7 3,402,727 - BT2746 0.66 -1.6 3,402,780 - BT2746 0.69 -1.1 3,402,874 - BT2746 0.75 +1.7 3,402,893 + BT2746 0.76 -2.0 3,402,894 - BT2746 0.76 -1.5 3,402,939 - BT2746 0.79 -1.2 3,403,008 + BT2746 0.83 -0.7 3,403,031 - BT2746 0.84 -1.2 3,403,053 + BT2746 0.86 -1.3 3,403,057 + BT2746 0.86 -2.0 3,403,057 + BT2746 0.86 -0.7 3,403,058 - BT2746 0.86 -0.9 3,403,099 + -1.2 3,403,100 - BT2746 0.89 +0.7 3,403,150 - -1.5 3,403,189 - -2.5 3,403,198 + -2.4 3,403,214 + -1.0 3,403,217 - -0.7 3,403,262 - -1.0 3,403,277 - -1.7 3,403,310 + -2.0 3,403,317 - -0.4 3,403,367 + +0.7 3,403,368 - -2.2 3,403,419 - -1.6 3,403,430 - -2.4 3,403,430 - -2.0 3,403,438 - -0.6 3,403,440 - -1.9 3,403,440 - -3.0
Or see this region's nucleotide sequence