Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1911 and BT1912 are separated by 619 nucleotides BT1912 and BT1913 are separated by 109 nucleotides
BT1911: BT1911 - 7-alpha-hydroxysteroid dehydrogenase (NCBI ptt file), at 2,400,048 to 2,400,827
BT1911
BT1912: BT1912 - hypothetical protein (NCBI ptt file), at 2,401,447 to 2,401,650
BT1912
BT1913: BT1913 - nicotinate phosphoribosyltransferase (NCBI ptt file), at 2,401,760 to 2,402,929
BT1913
Position (kb)
2401
2402 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2400.476 kb on - strand, within BT1911 at 2400.482 kb on + strand, within BT1911 at 2400.483 kb on - strand, within BT1911 at 2400.483 kb on - strand, within BT1911 at 2400.483 kb on - strand, within BT1911 at 2400.665 kb on + strand, within BT1911 at 2400.668 kb on - strand, within BT1911 at 2400.726 kb on - strand, within BT1911 at 2400.756 kb on + strand at 2400.756 kb on + strand at 2400.756 kb on + strand at 2400.825 kb on + strand at 2400.826 kb on - strand at 2400.826 kb on - strand at 2400.829 kb on + strand at 2400.829 kb on + strand at 2400.830 kb on - strand at 2400.830 kb on - strand at 2400.900 kb on - strand at 2400.915 kb on - strand at 2400.928 kb on + strand at 2400.978 kb on + strand at 2400.996 kb on - strand at 2401.023 kb on - strand at 2401.069 kb on - strand at 2401.103 kb on + strand at 2401.103 kb on + strand at 2401.103 kb on + strand at 2401.114 kb on + strand at 2401.243 kb on - strand at 2401.281 kb on + strand at 2401.341 kb on + strand at 2401.341 kb on + strand at 2401.342 kb on - strand at 2401.344 kb on + strand at 2401.345 kb on - strand at 2401.494 kb on + strand, within BT1912 at 2401.495 kb on - strand, within BT1912 at 2401.521 kb on + strand, within BT1912 at 2401.572 kb on + strand, within BT1912 at 2401.666 kb on - strand at 2401.743 kb on + strand at 2401.767 kb on + strand at 2401.768 kb on - strand at 2401.776 kb on + strand at 2401.794 kb on - strand at 2401.795 kb on + strand at 2401.795 kb on + strand at 2401.795 kb on + strand at 2401.795 kb on + strand at 2401.796 kb on - strand at 2401.796 kb on - strand at 2401.812 kb on - strand at 2401.817 kb on + strand at 2401.818 kb on - strand at 2401.818 kb on - strand at 2401.882 kb on + strand, within BT1913 at 2401.882 kb on + strand, within BT1913 at 2401.990 kb on + strand, within BT1913 at 2402.044 kb on + strand, within BT1913 at 2402.044 kb on + strand, within BT1913 at 2402.111 kb on + strand, within BT1913 at 2402.112 kb on - strand, within BT1913 at 2402.112 kb on - strand, within BT1913 at 2402.119 kb on - strand, within BT1913 at 2402.169 kb on + strand, within BT1913 at 2402.188 kb on - strand, within BT1913 at 2402.346 kb on - strand, within BT1913 at 2402.360 kb on + strand, within BT1913 at 2402.361 kb on - strand, within BT1913 at 2402.429 kb on - strand, within BT1913 at 2402.443 kb on + strand, within BT1913 at 2402.469 kb on + strand, within BT1913 at 2402.507 kb on + strand, within BT1913 at 2402.510 kb on - strand, within BT1913 at 2402.556 kb on + strand, within BT1913 at 2402.622 kb on - strand, within BT1913
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 2,400,476 - BT1911 0.55 +0.9 2,400,482 + BT1911 0.56 -2.3 2,400,483 - BT1911 0.56 +0.6 2,400,483 - BT1911 0.56 +0.3 2,400,483 - BT1911 0.56 -1.7 2,400,665 + BT1911 0.79 -0.7 2,400,668 - BT1911 0.79 -0.4 2,400,726 - BT1911 0.87 +1.7 2,400,756 + +0.3 2,400,756 + -1.2 2,400,756 + -0.8 2,400,825 + -0.6 2,400,826 - +0.8 2,400,826 - -3.4 2,400,829 + -0.2 2,400,829 + -1.2 2,400,830 - -1.0 2,400,830 - -0.6 2,400,900 - +0.4 2,400,915 - -0.3 2,400,928 + -1.2 2,400,978 + -0.6 2,400,996 - -1.6 2,401,023 - -3.1 2,401,069 - -0.4 2,401,103 + -2.7 2,401,103 + -1.0 2,401,103 + -1.8 2,401,114 + +0.6 2,401,243 - -2.0 2,401,281 + -0.9 2,401,341 + -1.4 2,401,341 + -3.0 2,401,342 - -3.2 2,401,344 + -1.2 2,401,345 - +0.8 2,401,494 + BT1912 0.23 -3.4 2,401,495 - BT1912 0.24 -3.5 2,401,521 + BT1912 0.36 -0.2 2,401,572 + BT1912 0.61 +1.4 2,401,666 - -1.9 2,401,743 + +1.4 2,401,767 + -1.7 2,401,768 - -2.5 2,401,776 + -0.6 2,401,794 - -1.8 2,401,795 + -1.4 2,401,795 + -1.3 2,401,795 + +1.4 2,401,795 + -1.6 2,401,796 - +0.7 2,401,796 - +0.4 2,401,812 - -1.2 2,401,817 + -1.4 2,401,818 - +0.1 2,401,818 - -1.2 2,401,882 + BT1913 0.10 +1.3 2,401,882 + BT1913 0.10 +0.1 2,401,990 + BT1913 0.20 -0.2 2,402,044 + BT1913 0.24 +0.1 2,402,044 + BT1913 0.24 -1.6 2,402,111 + BT1913 0.30 +2.2 2,402,112 - BT1913 0.30 -0.7 2,402,112 - BT1913 0.30 -2.4 2,402,119 - BT1913 0.31 -2.2 2,402,169 + BT1913 0.35 -0.7 2,402,188 - BT1913 0.37 -0.6 2,402,346 - BT1913 0.50 -0.6 2,402,360 + BT1913 0.51 -2.3 2,402,361 - BT1913 0.51 -2.5 2,402,429 - BT1913 0.57 +0.3 2,402,443 + BT1913 0.58 -1.1 2,402,469 + BT1913 0.61 -2.2 2,402,507 + BT1913 0.64 -0.3 2,402,510 - BT1913 0.64 +0.7 2,402,556 + BT1913 0.68 -2.5 2,402,622 - BT1913 0.74 -0.3
Or see this region's nucleotide sequence