Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1236 and BT1237 overlap by 4 nucleotides BT1237 and BT1238 are separated by 75 nucleotides
BT1236: BT1236 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase precursor (NCBI ptt file), at 1,537,247 to 1,538,989
BT1236
BT1237: BT1237 - two-component system sensor histidine kinase (NCBI ptt file), at 1,538,986 to 1,540,911
BT1237
BT1238: BT1238 - putative RNA binding protein with S1 RNA-binding domain (NCBI ptt file), at 1,540,987 to 1,543,161
BT1238
Position (kb)
1538
1539
1540
1541 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1538.006 kb on - strand, within BT1236 at 1538.019 kb on + strand, within BT1236 at 1538.041 kb on + strand, within BT1236 at 1538.246 kb on + strand, within BT1236 at 1538.301 kb on + strand, within BT1236 at 1538.302 kb on - strand, within BT1236 at 1538.521 kb on + strand, within BT1236 at 1538.521 kb on + strand, within BT1236 at 1538.522 kb on - strand, within BT1236 at 1538.553 kb on - strand, within BT1236 at 1538.566 kb on + strand, within BT1236 at 1538.662 kb on - strand, within BT1236 at 1538.690 kb on + strand, within BT1236 at 1538.691 kb on - strand, within BT1236 at 1538.773 kb on + strand, within BT1236 at 1538.787 kb on + strand, within BT1236 at 1538.865 kb on + strand at 1538.968 kb on - strand at 1539.090 kb on + strand at 1539.251 kb on - strand, within BT1237 at 1539.298 kb on - strand, within BT1237 at 1539.418 kb on + strand, within BT1237 at 1539.489 kb on + strand, within BT1237 at 1539.489 kb on + strand, within BT1237 at 1539.489 kb on + strand, within BT1237 at 1539.490 kb on - strand, within BT1237 at 1539.490 kb on - strand, within BT1237 at 1539.531 kb on + strand, within BT1237 at 1539.598 kb on + strand, within BT1237 at 1539.619 kb on - strand, within BT1237 at 1539.822 kb on + strand, within BT1237 at 1539.822 kb on + strand, within BT1237 at 1539.823 kb on - strand, within BT1237 at 1539.823 kb on - strand, within BT1237 at 1539.823 kb on - strand, within BT1237 at 1539.824 kb on + strand, within BT1237 at 1540.115 kb on + strand, within BT1237 at 1540.119 kb on + strand, within BT1237 at 1540.119 kb on + strand, within BT1237 at 1540.219 kb on - strand, within BT1237 at 1540.227 kb on + strand, within BT1237 at 1540.317 kb on - strand, within BT1237 at 1540.397 kb on + strand, within BT1237 at 1540.581 kb on - strand, within BT1237 at 1540.722 kb on + strand at 1540.749 kb on - strand at 1540.760 kb on + strand at 1540.812 kb on + strand at 1540.850 kb on - strand at 1540.884 kb on + strand at 1540.898 kb on - strand at 1540.899 kb on + strand at 1540.901 kb on + strand at 1540.901 kb on + strand at 1540.902 kb on - strand at 1540.902 kb on - strand at 1540.912 kb on - strand at 1540.956 kb on + strand at 1540.959 kb on - strand at 1540.987 kb on - strand at 1541.060 kb on + strand at 1541.332 kb on + strand, within BT1238 at 1541.639 kb on - strand, within BT1238 at 1541.780 kb on + strand, within BT1238 at 1541.810 kb on + strand, within BT1238
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 1,538,006 - BT1236 0.44 -0.6 1,538,019 + BT1236 0.44 -1.9 1,538,041 + BT1236 0.46 -1.7 1,538,246 + BT1236 0.57 -3.8 1,538,301 + BT1236 0.60 +1.8 1,538,302 - BT1236 0.61 -0.6 1,538,521 + BT1236 0.73 -0.6 1,538,521 + BT1236 0.73 -0.9 1,538,522 - BT1236 0.73 -2.8 1,538,553 - BT1236 0.75 +1.1 1,538,566 + BT1236 0.76 -1.4 1,538,662 - BT1236 0.81 -1.4 1,538,690 + BT1236 0.83 -0.6 1,538,691 - BT1236 0.83 -0.7 1,538,773 + BT1236 0.88 -2.7 1,538,787 + BT1236 0.88 -1.9 1,538,865 + -1.4 1,538,968 - -1.4 1,539,090 + -0.6 1,539,251 - BT1237 0.14 +1.2 1,539,298 - BT1237 0.16 +0.4 1,539,418 + BT1237 0.22 +2.3 1,539,489 + BT1237 0.26 +0.0 1,539,489 + BT1237 0.26 +0.6 1,539,489 + BT1237 0.26 +2.0 1,539,490 - BT1237 0.26 -3.1 1,539,490 - BT1237 0.26 -0.4 1,539,531 + BT1237 0.28 +0.8 1,539,598 + BT1237 0.32 +1.4 1,539,619 - BT1237 0.33 -2.9 1,539,822 + BT1237 0.43 +1.4 1,539,822 + BT1237 0.43 -0.1 1,539,823 - BT1237 0.43 -0.6 1,539,823 - BT1237 0.43 -1.6 1,539,823 - BT1237 0.43 -1.8 1,539,824 + BT1237 0.44 -1.5 1,540,115 + BT1237 0.59 -1.5 1,540,119 + BT1237 0.59 -0.4 1,540,119 + BT1237 0.59 +1.4 1,540,219 - BT1237 0.64 -2.2 1,540,227 + BT1237 0.64 -1.8 1,540,317 - BT1237 0.69 -1.6 1,540,397 + BT1237 0.73 +0.0 1,540,581 - BT1237 0.83 -0.2 1,540,722 + -0.2 1,540,749 - -2.9 1,540,760 + -2.8 1,540,812 + -1.8 1,540,850 - -0.6 1,540,884 + -1.0 1,540,898 - +1.0 1,540,899 + +3.6 1,540,901 + -2.2 1,540,901 + -1.0 1,540,902 - -1.4 1,540,902 - -0.8 1,540,912 - -0.9 1,540,956 + -0.6 1,540,959 - -1.6 1,540,987 - -2.5 1,541,060 + -0.2 1,541,332 + BT1238 0.16 -0.3 1,541,639 - BT1238 0.30 -0.0 1,541,780 + BT1238 0.36 -1.6 1,541,810 + BT1238 0.38 -2.0
Or see this region's nucleotide sequence