Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0566 and BT0567 are separated by 35 nucleotides BT0567 and BT0568 overlap by 4 nucleotides BT0568 and BT0569 are separated by 308 nucleotides
BT0566: BT0566 - alanyl-tRNA synthetase (NCBI ptt file), at 702,524 to 703,000
BT0566
BT0567: BT0567 - putative protein involved in barrier function of the cell envelope (NCBI ptt file), at 703,036 to 703,680
BT0567
BT0568: BT0568 - hypothetical protein (NCBI ptt file), at 703,677 to 704,345
BT0568
BT0569: BT0569 - conserved hypothetical protein (NCBI ptt file), at 704,654 to 705,364
BT0569
Position (kb) 703
704
705 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 702.731 kb on - strand, within BT0566 at 702.791 kb on + strand, within BT0566 at 702.797 kb on - strand, within BT0566 at 702.845 kb on - strand, within BT0566 at 702.847 kb on - strand, within BT0566 at 702.873 kb on - strand, within BT0566 at 702.874 kb on + strand, within BT0566 at 702.916 kb on + strand, within BT0566 at 702.943 kb on + strand, within BT0566 at 702.944 kb on - strand, within BT0566 at 702.949 kb on + strand, within BT0566 at 702.950 kb on - strand, within BT0566 at 702.950 kb on - strand, within BT0566 at 703.037 kb on + strand at 703.086 kb on + strand at 703.086 kb on + strand at 703.087 kb on - strand at 703.087 kb on - strand at 703.127 kb on - strand, within BT0567 at 703.166 kb on - strand, within BT0567 at 703.277 kb on - strand, within BT0567 at 703.395 kb on - strand, within BT0567 at 703.428 kb on + strand, within BT0567 at 703.541 kb on - strand, within BT0567 at 703.558 kb on - strand, within BT0567 at 703.574 kb on + strand, within BT0567 at 703.617 kb on + strand at 703.648 kb on + strand at 703.649 kb on - strand at 703.661 kb on + strand at 703.747 kb on + strand, within BT0568 at 703.761 kb on - strand, within BT0568 at 703.784 kb on + strand, within BT0568 at 703.842 kb on + strand, within BT0568 at 703.901 kb on + strand, within BT0568 at 703.914 kb on - strand, within BT0568 at 703.916 kb on + strand, within BT0568 at 703.995 kb on - strand, within BT0568 at 703.998 kb on - strand, within BT0568 at 704.053 kb on + strand, within BT0568 at 704.123 kb on - strand, within BT0568 at 704.123 kb on - strand, within BT0568 at 704.149 kb on + strand, within BT0568 at 704.149 kb on + strand, within BT0568 at 704.150 kb on - strand, within BT0568 at 704.151 kb on + strand, within BT0568 at 704.152 kb on - strand, within BT0568 at 704.272 kb on - strand, within BT0568 at 704.640 kb on - strand at 704.657 kb on + strand at 704.658 kb on - strand at 704.760 kb on - strand, within BT0569 at 704.935 kb on - strand, within BT0569 at 704.986 kb on + strand, within BT0569 at 705.026 kb on + strand, within BT0569 at 705.026 kb on + strand, within BT0569 at 705.040 kb on + strand, within BT0569 at 705.040 kb on + strand, within BT0569 at 705.062 kb on - strand, within BT0569 at 705.149 kb on - strand, within BT0569 at 705.190 kb on - strand, within BT0569 at 705.211 kb on + strand, within BT0569 at 705.281 kb on - strand, within BT0569 at 705.294 kb on + strand at 705.319 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 702,731 - BT0566 0.43 -0.1 702,791 + BT0566 0.56 -0.2 702,797 - BT0566 0.57 -0.9 702,845 - BT0566 0.67 -0.9 702,847 - BT0566 0.68 -0.5 702,873 - BT0566 0.73 -0.5 702,874 + BT0566 0.73 -1.1 702,916 + BT0566 0.82 -0.9 702,943 + BT0566 0.88 -0.3 702,944 - BT0566 0.88 -2.1 702,949 + BT0566 0.89 +0.1 702,950 - BT0566 0.89 -2.0 702,950 - BT0566 0.89 +0.6 703,037 + -1.0 703,086 + -0.9 703,086 + -0.9 703,087 - -1.4 703,087 - +0.1 703,127 - BT0567 0.14 +1.5 703,166 - BT0567 0.20 +0.1 703,277 - BT0567 0.37 -1.9 703,395 - BT0567 0.56 -1.7 703,428 + BT0567 0.61 -0.5 703,541 - BT0567 0.78 -1.4 703,558 - BT0567 0.81 -3.5 703,574 + BT0567 0.83 +0.5 703,617 + +0.9 703,648 + -2.4 703,649 - -2.4 703,661 + -0.1 703,747 + BT0568 0.10 -2.6 703,761 - BT0568 0.13 -1.0 703,784 + BT0568 0.16 -1.3 703,842 + BT0568 0.25 -4.1 703,901 + BT0568 0.33 -2.6 703,914 - BT0568 0.35 +2.4 703,916 + BT0568 0.36 +0.4 703,995 - BT0568 0.48 -0.7 703,998 - BT0568 0.48 -0.1 704,053 + BT0568 0.56 +1.0 704,123 - BT0568 0.67 +0.9 704,123 - BT0568 0.67 -0.6 704,149 + BT0568 0.71 +1.0 704,149 + BT0568 0.71 -2.0 704,150 - BT0568 0.71 +0.9 704,151 + BT0568 0.71 -2.0 704,152 - BT0568 0.71 +0.4 704,272 - BT0568 0.89 +0.4 704,640 - +1.4 704,657 + +0.0 704,658 - +0.4 704,760 - BT0569 0.15 -0.1 704,935 - BT0569 0.40 -1.9 704,986 + BT0569 0.47 -2.5 705,026 + BT0569 0.52 -1.1 705,026 + BT0569 0.52 -1.1 705,040 + BT0569 0.54 -0.1 705,040 + BT0569 0.54 -0.8 705,062 - BT0569 0.57 +0.7 705,149 - BT0569 0.70 -1.1 705,190 - BT0569 0.75 -3.0 705,211 + BT0569 0.78 -0.6 705,281 - BT0569 0.88 -2.3 705,294 + -0.6 705,319 + -0.6
Or see this region's nucleotide sequence