Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0374

Experiment: BHIS with Simvastatin 30 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0373 and BT0374 are separated by 148 nucleotidesBT0374 and BT0375 are separated by 369 nucleotides BT0373: BT0373 - mannose-6-phosphate isomerase (NCBI ptt file), at 459,328 to 460,299 BT0373 BT0374: BT0374 - conserved hypothetical protein (NCBI ptt file), at 460,448 to 461,998 BT0374 BT0375: BT0375 - integrase (NCBI ptt file), at 462,368 to 463,057 BT0375 Position (kb) 460 461 462Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 460.312 kb on - strandat 460.320 kb on + strandat 460.408 kb on + strandat 460.408 kb on + strandat 460.430 kb on + strandat 461.551 kb on + strand, within BT0374at 461.574 kb on - strand, within BT0374at 461.583 kb on + strand, within BT0374at 461.593 kb on - strand, within BT0374at 461.620 kb on + strand, within BT0374at 461.723 kb on + strand, within BT0374at 461.996 kb on + strandat 462.082 kb on - strandat 462.106 kb on + strandat 462.108 kb on + strandat 462.117 kb on - strandat 462.148 kb on - strandat 462.183 kb on - strandat 462.291 kb on + strandat 462.334 kb on - strandat 462.334 kb on - strandat 462.334 kb on - strandat 462.480 kb on - strand, within BT0375at 462.659 kb on - strand, within BT0375at 462.774 kb on - strand, within BT0375at 462.864 kb on - strand, within BT0375at 462.864 kb on - strand, within BT0375at 462.864 kb on - strand, within BT0375at 462.865 kb on + strand, within BT0375at 462.865 kb on + strand, within BT0375at 462.868 kb on + strand, within BT0375at 462.887 kb on - strand, within BT0375at 462.889 kb on + strand, within BT0375at 462.944 kb on - strand, within BT0375at 462.998 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM
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460,312 - -2.0
460,320 + +2.4
460,408 + +0.6
460,408 + -0.2
460,430 + -1.2
461,551 + BT0374 0.71 -1.9
461,574 - BT0374 0.73 +1.4
461,583 + BT0374 0.73 -2.4
461,593 - BT0374 0.74 +0.3
461,620 + BT0374 0.76 +0.7
461,723 + BT0374 0.82 -2.5
461,996 + -0.6
462,082 - -4.0
462,106 + -1.6
462,108 + +0.2
462,117 - -1.2
462,148 - -3.4
462,183 - -0.1
462,291 + -0.9
462,334 - -3.0
462,334 - -1.0
462,334 - -0.4
462,480 - BT0375 0.16 -3.2
462,659 - BT0375 0.42 +1.7
462,774 - BT0375 0.59 -1.2
462,864 - BT0375 0.72 -2.0
462,864 - BT0375 0.72 -1.4
462,864 - BT0375 0.72 +0.1
462,865 + BT0375 0.72 +0.4
462,865 + BT0375 0.72 -3.2
462,868 + BT0375 0.72 +0.4
462,887 - BT0375 0.75 -1.2
462,889 + BT0375 0.76 -1.4
462,944 - BT0375 0.83 -1.6
462,998 - -0.2

Or see this region's nucleotide sequence