Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0300 and BT0301 are separated by 4 nucleotides BT0301 and BT0302 are separated by 15 nucleotides BT0302 and BT0303 are separated by 149 nucleotides
BT0300: BT0300 - cation efflux system (AcrB/AcrD/AcrF family) (NCBI ptt file), at 354,807 to 358,001
BT0300
BT0301: BT0301 - ATP-binding transport protein natA (Na+ ABC transporter) (NCBI ptt file), at 358,006 to 359,517
BT0301
BT0302: BT0302 - hypothetical protein (NCBI ptt file), at 359,533 to 360,234
BT0302
BT0303: BT0303 - putative patatin-like phospholipase (NCBI ptt file), at 360,384 to 362,600
BT0303
Position (kb) 358
359
360 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 357.067 kb on - strand, within BT0300 at 357.209 kb on + strand, within BT0300 at 357.280 kb on + strand at 357.281 kb on - strand, within BT0300 at 357.369 kb on - strand, within BT0300 at 357.461 kb on - strand, within BT0300 at 357.464 kb on + strand, within BT0300 at 357.465 kb on - strand, within BT0300 at 357.493 kb on + strand, within BT0300 at 357.493 kb on + strand, within BT0300 at 357.557 kb on - strand, within BT0300 at 357.650 kb on - strand, within BT0300 at 357.655 kb on - strand, within BT0300 at 357.828 kb on - strand at 357.977 kb on + strand at 357.995 kb on - strand at 358.093 kb on - strand at 358.278 kb on + strand, within BT0301 at 358.279 kb on - strand, within BT0301 at 358.306 kb on + strand, within BT0301 at 358.354 kb on - strand, within BT0301 at 358.397 kb on - strand, within BT0301 at 358.402 kb on + strand, within BT0301 at 358.405 kb on + strand, within BT0301 at 358.506 kb on + strand, within BT0301 at 358.507 kb on - strand, within BT0301 at 358.579 kb on + strand, within BT0301 at 358.596 kb on + strand, within BT0301 at 358.596 kb on + strand, within BT0301 at 358.597 kb on - strand, within BT0301 at 358.597 kb on - strand, within BT0301 at 358.597 kb on - strand, within BT0301 at 358.598 kb on + strand, within BT0301 at 358.731 kb on + strand, within BT0301 at 358.731 kb on + strand, within BT0301 at 358.810 kb on + strand, within BT0301 at 358.811 kb on - strand, within BT0301 at 358.849 kb on + strand, within BT0301 at 358.850 kb on - strand, within BT0301 at 358.850 kb on - strand, within BT0301 at 358.936 kb on + strand, within BT0301 at 358.994 kb on - strand, within BT0301 at 359.013 kb on + strand, within BT0301 at 359.017 kb on + strand, within BT0301 at 359.058 kb on + strand, within BT0301 at 359.071 kb on - strand, within BT0301 at 359.099 kb on + strand, within BT0301 at 359.106 kb on + strand, within BT0301 at 359.217 kb on + strand, within BT0301 at 359.229 kb on + strand, within BT0301 at 359.230 kb on - strand, within BT0301 at 359.347 kb on - strand, within BT0301 at 359.388 kb on - strand at 359.395 kb on - strand at 359.447 kb on - strand at 359.488 kb on + strand at 359.750 kb on + strand, within BT0302 at 359.815 kb on - strand, within BT0302 at 359.819 kb on - strand, within BT0302 at 359.821 kb on - strand, within BT0302 at 359.901 kb on - strand, within BT0302 at 359.979 kb on + strand, within BT0302 at 359.979 kb on + strand, within BT0302 at 360.020 kb on + strand, within BT0302 at 360.021 kb on - strand, within BT0302 at 360.030 kb on + strand, within BT0302 at 360.346 kb on - strand at 360.350 kb on - strand at 360.351 kb on + strand at 360.362 kb on - strand at 360.386 kb on - strand at 360.394 kb on + strand at 360.471 kb on - strand at 360.479 kb on + strand at 360.479 kb on + strand at 360.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 357,067 - BT0300 0.71 -1.2 357,209 + BT0300 0.75 +1.4 357,280 + -0.9 357,281 - BT0300 0.77 -1.2 357,369 - BT0300 0.80 -1.6 357,461 - BT0300 0.83 -1.6 357,464 + BT0300 0.83 -0.1 357,465 - BT0300 0.83 -2.5 357,493 + BT0300 0.84 -1.4 357,493 + BT0300 0.84 -0.6 357,557 - BT0300 0.86 +0.3 357,650 - BT0300 0.89 +1.5 357,655 - BT0300 0.89 -1.1 357,828 - +0.4 357,977 + -0.9 357,995 - -0.9 358,093 - +0.5 358,278 + BT0301 0.18 -2.4 358,279 - BT0301 0.18 -0.1 358,306 + BT0301 0.20 -2.1 358,354 - BT0301 0.23 -1.5 358,397 - BT0301 0.26 -1.9 358,402 + BT0301 0.26 +0.5 358,405 + BT0301 0.26 -1.7 358,506 + BT0301 0.33 -0.4 358,507 - BT0301 0.33 -1.1 358,579 + BT0301 0.38 -2.1 358,596 + BT0301 0.39 +0.5 358,596 + BT0301 0.39 -0.9 358,597 - BT0301 0.39 -0.9 358,597 - BT0301 0.39 -0.5 358,597 - BT0301 0.39 +1.0 358,598 + BT0301 0.39 -2.0 358,731 + BT0301 0.48 -0.5 358,731 + BT0301 0.48 -1.4 358,810 + BT0301 0.53 +1.0 358,811 - BT0301 0.53 +0.1 358,849 + BT0301 0.56 -3.6 358,850 - BT0301 0.56 +0.0 358,850 - BT0301 0.56 -2.9 358,936 + BT0301 0.62 -1.7 358,994 - BT0301 0.65 +0.3 359,013 + BT0301 0.67 -1.0 359,017 + BT0301 0.67 -0.1 359,058 + BT0301 0.70 -0.9 359,071 - BT0301 0.70 -1.0 359,099 + BT0301 0.72 -0.1 359,106 + BT0301 0.73 +0.5 359,217 + BT0301 0.80 -0.9 359,229 + BT0301 0.81 -2.0 359,230 - BT0301 0.81 +0.5 359,347 - BT0301 0.89 +0.4 359,388 - -0.4 359,395 - +0.6 359,447 - +1.5 359,488 + -2.1 359,750 + BT0302 0.31 -1.7 359,815 - BT0302 0.40 -2.9 359,819 - BT0302 0.41 -0.5 359,821 - BT0302 0.41 -1.4 359,901 - BT0302 0.52 +1.5 359,979 + BT0302 0.64 -1.7 359,979 + BT0302 0.64 -2.2 360,020 + BT0302 0.69 -3.2 360,021 - BT0302 0.70 -0.9 360,030 + BT0302 0.71 -3.5 360,346 - -1.1 360,350 - -4.1 360,351 + -0.1 360,362 - -0.1 360,386 - -0.3 360,394 + -0.9 360,471 - -0.1 360,479 + -0.5 360,479 + -0.5 360,498 - -3.2
Or see this region's nucleotide sequence