Experiment: Nalidixic 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0006 and Psest_0007 are separated by 70 nucleotides Psest_0007 and Psest_0008 are separated by 50 nucleotides Psest_0008 and Psest_0009 overlap by 4 nucleotides
Psest_0006: Psest_0006 - alkyl hydroperoxide reductase, F subunit, at 7,009 to 8,571
_0006
Psest_0007: Psest_0007 - diguanylate cyclase (GGDEF) domain, at 8,642 to 10,012
_0007
Psest_0008: Psest_0008 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 10,063 to 10,812
_0008
Psest_0009: Psest_0009 - histidinol-phosphate phosphatase family domain/HAD-superfamily hydrolase, subfamily IIIA, at 10,809 to 11,387
_0009
Position (kb)
8
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 7.693 kb on + strand, within Psest_0006 at 7.694 kb on - strand, within Psest_0006 at 7.844 kb on - strand, within Psest_0006 at 8.287 kb on + strand, within Psest_0006 at 8.287 kb on + strand, within Psest_0006 at 8.287 kb on + strand, within Psest_0006 at 8.287 kb on + strand, within Psest_0006 at 8.627 kb on + strand at 8.627 kb on + strand at 8.692 kb on - strand at 8.727 kb on + strand at 8.728 kb on - strand at 8.728 kb on - strand at 8.988 kb on + strand, within Psest_0007 at 8.989 kb on - strand, within Psest_0007 at 8.989 kb on - strand, within Psest_0007 at 8.989 kb on - strand, within Psest_0007 at 8.989 kb on - strand, within Psest_0007 at 9.083 kb on + strand, within Psest_0007 at 9.083 kb on + strand, within Psest_0007 at 9.247 kb on + strand, within Psest_0007 at 9.281 kb on + strand, within Psest_0007 at 9.281 kb on + strand, within Psest_0007 at 9.281 kb on + strand, within Psest_0007 at 9.281 kb on + strand, within Psest_0007 at 9.282 kb on - strand, within Psest_0007 at 9.282 kb on - strand, within Psest_0007 at 9.282 kb on - strand, within Psest_0007 at 9.282 kb on - strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.285 kb on + strand, within Psest_0007 at 9.286 kb on - strand, within Psest_0007 at 9.286 kb on - strand, within Psest_0007 at 9.286 kb on - strand, within Psest_0007 at 9.544 kb on - strand, within Psest_0007 at 9.883 kb on - strand at 9.883 kb on - strand at 10.001 kb on + strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.013 kb on - strand at 10.828 kb on - strand at 10.828 kb on - strand at 10.828 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.00390625 mg/ml remove 7,693 + Psest_0006 0.44 -0.6 7,694 - Psest_0006 0.44 -1.5 7,844 - Psest_0006 0.53 +1.7 8,287 + Psest_0006 0.82 +0.1 8,287 + Psest_0006 0.82 -0.2 8,287 + Psest_0006 0.82 +0.6 8,287 + Psest_0006 0.82 -0.8 8,627 + -0.7 8,627 + -0.7 8,692 - -1.1 8,727 + -0.6 8,728 - -1.0 8,728 - -0.3 8,988 + Psest_0007 0.25 +2.2 8,989 - Psest_0007 0.25 +0.8 8,989 - Psest_0007 0.25 +0.1 8,989 - Psest_0007 0.25 -0.1 8,989 - Psest_0007 0.25 -1.0 9,083 + Psest_0007 0.32 -0.6 9,083 + Psest_0007 0.32 -1.4 9,247 + Psest_0007 0.44 -0.3 9,281 + Psest_0007 0.47 +1.3 9,281 + Psest_0007 0.47 +0.7 9,281 + Psest_0007 0.47 +2.0 9,281 + Psest_0007 0.47 +1.7 9,282 - Psest_0007 0.47 -0.0 9,282 - Psest_0007 0.47 -1.8 9,282 - Psest_0007 0.47 -0.3 9,282 - Psest_0007 0.47 +2.0 9,285 + Psest_0007 0.47 -0.3 9,285 + Psest_0007 0.47 +0.8 9,285 + Psest_0007 0.47 -1.4 9,285 + Psest_0007 0.47 +2.4 9,285 + Psest_0007 0.47 +0.1 9,285 + Psest_0007 0.47 -0.6 9,285 + Psest_0007 0.47 -1.1 9,286 - Psest_0007 0.47 -0.0 9,286 - Psest_0007 0.47 -0.3 9,286 - Psest_0007 0.47 -0.5 9,544 - Psest_0007 0.66 -0.7 9,883 - -1.3 9,883 - +1.2 10,001 + -1.4 10,002 - +0.8 10,002 - -0.8 10,002 - +0.7 10,002 - -0.5 10,002 - -0.8 10,013 - -1.5 10,828 - +0.2 10,828 - -1.2 10,828 - +0.5
Or see this region's nucleotide sequence