Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0663 and Psest_0664 are separated by 143 nucleotides Psest_0664 and Psest_0665 are separated by 84 nucleotides Psest_0665 and Psest_0666 are separated by 105 nucleotides
Psest_0663: Psest_0663 - PAS fold., at 721,219 to 723,018
_0663
Psest_0664: Psest_0664 - diguanylate cyclase (GGDEF) domain, at 723,162 to 724,652
_0664
Psest_0665: Psest_0665 - Inorganic pyrophosphatase, at 724,737 to 725,264
_0665
Psest_0666: Psest_0666 - Uncharacterized protein conserved in bacteria, at 725,370 to 726,182
_0666
Position (kb) 723
724
725 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 722.176 kb on + strand, within Psest_0663 at 722.176 kb on + strand, within Psest_0663 at 722.176 kb on + strand, within Psest_0663 at 722.176 kb on + strand, within Psest_0663 at 722.177 kb on - strand, within Psest_0663 at 722.177 kb on - strand, within Psest_0663 at 722.177 kb on - strand, within Psest_0663 at 722.401 kb on - strand, within Psest_0663 at 722.567 kb on + strand, within Psest_0663 at 722.626 kb on - strand, within Psest_0663 at 722.666 kb on + strand, within Psest_0663 at 722.761 kb on + strand, within Psest_0663 at 722.872 kb on + strand at 722.905 kb on + strand at 722.906 kb on - strand at 723.024 kb on + strand at 723.197 kb on - strand at 723.237 kb on - strand at 723.427 kb on - strand, within Psest_0664 at 723.601 kb on + strand, within Psest_0664 at 723.601 kb on + strand, within Psest_0664 at 723.602 kb on - strand, within Psest_0664 at 723.702 kb on + strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.837 kb on + strand, within Psest_0664 at 723.838 kb on - strand, within Psest_0664 at 723.838 kb on - strand, within Psest_0664 at 723.870 kb on + strand, within Psest_0664 at 723.870 kb on + strand, within Psest_0664 at 723.870 kb on + strand, within Psest_0664 at 723.871 kb on - strand, within Psest_0664 at 723.871 kb on - strand, within Psest_0664 at 723.871 kb on - strand, within Psest_0664 at 723.947 kb on - strand, within Psest_0664 at 724.490 kb on - strand, within Psest_0664 at 724.548 kb on + strand at 724.548 kb on + strand at 724.549 kb on - strand at 724.576 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.611 kb on + strand at 724.611 kb on + strand at 724.738 kb on + strand at 724.738 kb on + strand at 724.738 kb on + strand at 724.739 kb on - strand at 724.739 kb on - strand at 725.326 kb on - strand at 725.371 kb on + strand at 725.372 kb on - strand at 725.461 kb on + strand, within Psest_0666 at 725.462 kb on - strand, within Psest_0666 at 725.475 kb on + strand, within Psest_0666 at 725.475 kb on + strand, within Psest_0666 at 725.476 kb on - strand, within Psest_0666 at 725.476 kb on - strand, within Psest_0666 at 725.509 kb on + strand, within Psest_0666 at 725.509 kb on + strand, within Psest_0666 at 725.510 kb on - strand, within Psest_0666 at 725.614 kb on + strand, within Psest_0666
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 722,176 + Psest_0663 0.53 -0.7 722,176 + Psest_0663 0.53 -0.6 722,176 + Psest_0663 0.53 +0.4 722,176 + Psest_0663 0.53 -2.3 722,177 - Psest_0663 0.53 -0.9 722,177 - Psest_0663 0.53 -2.2 722,177 - Psest_0663 0.53 +0.3 722,401 - Psest_0663 0.66 -0.9 722,567 + Psest_0663 0.75 -1.3 722,626 - Psest_0663 0.78 -0.2 722,666 + Psest_0663 0.80 +0.0 722,761 + Psest_0663 0.86 +1.5 722,872 + +1.0 722,905 + -0.8 722,906 - +2.0 723,024 + +0.3 723,197 - +0.0 723,237 - -3.0 723,427 - Psest_0664 0.18 -1.1 723,601 + Psest_0664 0.29 +0.2 723,601 + Psest_0664 0.29 +0.4 723,602 - Psest_0664 0.30 +0.2 723,702 + Psest_0664 0.36 +0.9 723,703 - Psest_0664 0.36 +0.2 723,703 - Psest_0664 0.36 -0.2 723,703 - Psest_0664 0.36 -2.2 723,703 - Psest_0664 0.36 +0.7 723,837 + Psest_0664 0.45 +0.0 723,838 - Psest_0664 0.45 +2.3 723,838 - Psest_0664 0.45 -1.6 723,870 + Psest_0664 0.47 -3.2 723,870 + Psest_0664 0.47 -1.4 723,870 + Psest_0664 0.47 -0.2 723,871 - Psest_0664 0.48 -1.7 723,871 - Psest_0664 0.48 +0.1 723,871 - Psest_0664 0.48 -0.7 723,947 - Psest_0664 0.53 -1.3 724,490 - Psest_0664 0.89 -2.0 724,548 + +1.0 724,548 + +1.2 724,549 - +0.5 724,576 + -1.3 724,578 + +0.3 724,578 + -1.4 724,578 + -1.3 724,578 + -0.4 724,578 + -2.4 724,579 - -1.0 724,579 - +0.3 724,579 - +0.7 724,579 - -0.7 724,579 - -1.9 724,611 + +0.3 724,611 + -0.1 724,738 + +0.4 724,738 + +0.7 724,738 + -0.4 724,739 - +0.8 724,739 - -0.6 725,326 - -1.2 725,371 + +1.8 725,372 - -0.2 725,461 + Psest_0666 0.11 -0.7 725,462 - Psest_0666 0.11 +1.4 725,475 + Psest_0666 0.13 +0.1 725,475 + Psest_0666 0.13 -2.1 725,476 - Psest_0666 0.13 -1.6 725,476 - Psest_0666 0.13 -1.4 725,509 + Psest_0666 0.17 +0.1 725,509 + Psest_0666 0.17 -0.2 725,510 - Psest_0666 0.17 -0.2 725,614 + Psest_0666 0.30 -0.5
Or see this region's nucleotide sequence