Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0495 and Psest_0496 are separated by 164 nucleotides Psest_0496 and Psest_0497 are separated by 9 nucleotides Psest_0497 and Psest_0498 are separated by 9 nucleotides
Psest_0495: Psest_0495 - HDOD domain., at 549,397 to 550,911
_0495
Psest_0496: Psest_0496 - flagellar motor stator protein MotA, at 551,076 to 551,927
_0496
Psest_0497: Psest_0497 - Flagellar motor protein, at 551,937 to 552,917
_0497
Psest_0498: Psest_0498 - ribosome small subunit-dependent GTPase A, at 552,927 to 553,958
_0498
Position (kb) 551
552 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 550.727 kb on - strand, within Psest_0495 at 550.892 kb on - strand at 551.069 kb on + strand at 551.069 kb on + strand at 551.070 kb on - strand at 551.070 kb on - strand at 551.075 kb on + strand at 551.075 kb on + strand at 551.075 kb on + strand at 551.075 kb on + strand at 551.076 kb on - strand at 551.293 kb on - strand, within Psest_0496 at 551.293 kb on - strand, within Psest_0496 at 551.293 kb on - strand, within Psest_0496 at 551.295 kb on + strand, within Psest_0496 at 551.295 kb on + strand, within Psest_0496 at 551.295 kb on + strand, within Psest_0496 at 551.320 kb on - strand, within Psest_0496 at 551.409 kb on + strand, within Psest_0496 at 551.409 kb on + strand, within Psest_0496 at 551.410 kb on - strand, within Psest_0496 at 551.410 kb on - strand, within Psest_0496 at 551.410 kb on - strand, within Psest_0496 at 551.457 kb on + strand, within Psest_0496 at 551.457 kb on + strand, within Psest_0496 at 551.458 kb on - strand, within Psest_0496 at 551.458 kb on - strand, within Psest_0496 at 551.458 kb on - strand, within Psest_0496 at 551.673 kb on + strand, within Psest_0496 at 551.725 kb on - strand, within Psest_0496 at 551.725 kb on - strand, within Psest_0496 at 551.725 kb on - strand, within Psest_0496 at 551.925 kb on + strand at 551.926 kb on - strand at 552.101 kb on + strand, within Psest_0497 at 552.101 kb on + strand, within Psest_0497 at 552.102 kb on - strand, within Psest_0497 at 552.112 kb on - strand, within Psest_0497 at 552.459 kb on + strand, within Psest_0497 at 552.459 kb on + strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.491 kb on + strand, within Psest_0497 at 552.492 kb on - strand, within Psest_0497 at 552.722 kb on + strand, within Psest_0497 at 552.826 kb on + strand at 552.838 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 550,727 - Psest_0495 0.88 -0.3 550,892 - +1.5 551,069 + -2.2 551,069 + -0.0 551,070 - -2.4 551,070 - -0.0 551,075 + -0.7 551,075 + -0.1 551,075 + +2.1 551,075 + -0.2 551,076 - +0.1 551,293 - Psest_0496 0.25 +0.3 551,293 - Psest_0496 0.25 -0.5 551,293 - Psest_0496 0.25 -1.1 551,295 + Psest_0496 0.26 +1.9 551,295 + Psest_0496 0.26 +0.3 551,295 + Psest_0496 0.26 +0.6 551,320 - Psest_0496 0.29 +0.2 551,409 + Psest_0496 0.39 -0.5 551,409 + Psest_0496 0.39 -0.2 551,410 - Psest_0496 0.39 +0.3 551,410 - Psest_0496 0.39 +1.0 551,410 - Psest_0496 0.39 +0.1 551,457 + Psest_0496 0.45 -1.6 551,457 + Psest_0496 0.45 +0.5 551,458 - Psest_0496 0.45 +0.4 551,458 - Psest_0496 0.45 -0.5 551,458 - Psest_0496 0.45 +0.4 551,673 + Psest_0496 0.70 -2.5 551,725 - Psest_0496 0.76 +0.6 551,725 - Psest_0496 0.76 +2.1 551,725 - Psest_0496 0.76 -0.2 551,925 + -3.5 551,926 - +0.4 552,101 + Psest_0497 0.17 -0.9 552,101 + Psest_0497 0.17 -1.2 552,102 - Psest_0497 0.17 +0.0 552,112 - Psest_0497 0.18 -0.5 552,459 + Psest_0497 0.53 -0.3 552,459 + Psest_0497 0.53 +1.9 552,460 - Psest_0497 0.53 -0.7 552,460 - Psest_0497 0.53 +1.4 552,460 - Psest_0497 0.53 +0.3 552,460 - Psest_0497 0.53 +0.5 552,491 + Psest_0497 0.56 +0.1 552,492 - Psest_0497 0.57 +0.2 552,722 + Psest_0497 0.80 -1.2 552,826 + -0.4 552,838 + +0.8
Or see this region's nucleotide sequence