Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3238 and Psest_3239 are separated by 353 nucleotides Psest_3239 and Psest_3240 are separated by 190 nucleotides Psest_3240 and Psest_3241 are separated by 43 nucleotides
Psest_3238: Psest_3238 - malate:quinone-oxidoreductase, at 3,408,135 to 3,409,625
_3238
Psest_3239: Psest_3239 - hypothetical protein, at 3,409,979 to 3,410,266
_3239
Psest_3240: Psest_3240 - Sulfate permease and related transporters (MFS superfamily), at 3,410,457 to 3,412,187
_3240
Psest_3241: Psest_3241 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 3,412,231 to 3,413,142
_3241
Position (kb)
3410
3411
3412
3413 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3409.653 kb on - strand at 3409.653 kb on - strand at 3409.653 kb on - strand at 3409.653 kb on - strand at 3409.653 kb on - strand at 3409.684 kb on - strand at 3410.011 kb on - strand, within Psest_3239 at 3410.011 kb on - strand, within Psest_3239 at 3410.011 kb on - strand, within Psest_3239 at 3410.011 kb on - strand, within Psest_3239 at 3410.011 kb on - strand, within Psest_3239 at 3410.011 kb on - strand, within Psest_3239 at 3410.048 kb on - strand, within Psest_3239 at 3410.360 kb on + strand at 3410.360 kb on + strand at 3410.360 kb on + strand at 3410.360 kb on + strand at 3410.360 kb on + strand at 3410.361 kb on - strand at 3410.361 kb on - strand at 3410.361 kb on - strand at 3410.361 kb on - strand at 3410.361 kb on - strand at 3410.369 kb on - strand at 3410.402 kb on + strand at 3410.402 kb on + strand at 3410.439 kb on + strand at 3410.440 kb on - strand at 3410.625 kb on + strand at 3410.625 kb on + strand at 3410.633 kb on - strand, within Psest_3240 at 3410.937 kb on + strand, within Psest_3240 at 3410.937 kb on + strand, within Psest_3240 at 3410.937 kb on + strand, within Psest_3240 at 3410.938 kb on - strand, within Psest_3240 at 3410.938 kb on - strand, within Psest_3240 at 3410.938 kb on - strand, within Psest_3240 at 3411.055 kb on + strand, within Psest_3240 at 3411.055 kb on + strand, within Psest_3240 at 3411.153 kb on + strand, within Psest_3240 at 3411.154 kb on - strand, within Psest_3240 at 3411.561 kb on + strand, within Psest_3240 at 3411.562 kb on - strand, within Psest_3240 at 3411.562 kb on - strand, within Psest_3240 at 3411.667 kb on + strand, within Psest_3240 at 3411.667 kb on + strand, within Psest_3240 at 3411.667 kb on + strand, within Psest_3240 at 3411.668 kb on - strand, within Psest_3240 at 3411.668 kb on - strand, within Psest_3240 at 3411.849 kb on + strand, within Psest_3240 at 3412.110 kb on + strand at 3412.110 kb on + strand at 3412.110 kb on + strand at 3412.110 kb on + strand at 3412.110 kb on + strand at 3412.110 kb on + strand at 3412.110 kb on + strand at 3412.110 kb on + strand at 3412.110 kb on + strand at 3412.111 kb on - strand at 3412.111 kb on - strand at 3412.111 kb on - strand at 3412.111 kb on - strand at 3412.111 kb on - strand at 3412.156 kb on + strand at 3412.472 kb on + strand, within Psest_3241 at 3412.472 kb on + strand, within Psest_3241 at 3412.472 kb on + strand, within Psest_3241 at 3412.473 kb on - strand, within Psest_3241 at 3412.473 kb on - strand, within Psest_3241 at 3412.473 kb on - strand, within Psest_3241 at 3412.473 kb on - strand, within Psest_3241 at 3412.473 kb on - strand, within Psest_3241 at 3412.628 kb on + strand, within Psest_3241 at 3412.653 kb on - strand, within Psest_3241 at 3412.676 kb on + strand, within Psest_3241 at 3412.677 kb on - strand, within Psest_3241 at 3412.677 kb on - strand, within Psest_3241 at 3412.677 kb on - strand, within Psest_3241 at 3412.680 kb on + strand, within Psest_3241 at 3412.705 kb on + strand, within Psest_3241 at 3412.715 kb on + strand, within Psest_3241 at 3412.715 kb on + strand, within Psest_3241 at 3412.715 kb on + strand, within Psest_3241 at 3412.715 kb on + strand, within Psest_3241 at 3412.716 kb on - strand, within Psest_3241 at 3412.716 kb on - strand, within Psest_3241 at 3412.732 kb on - strand, within Psest_3241 at 3412.732 kb on - strand, within Psest_3241 at 3412.760 kb on + strand, within Psest_3241 at 3412.760 kb on + strand, within Psest_3241 at 3412.760 kb on + strand, within Psest_3241 at 3412.761 kb on - strand, within Psest_3241 at 3412.761 kb on - strand, within Psest_3241 at 3412.761 kb on - strand, within Psest_3241 at 3412.761 kb on - strand, within Psest_3241 at 3412.761 kb on - strand, within Psest_3241 at 3412.874 kb on + strand, within Psest_3241 at 3412.875 kb on - strand, within Psest_3241 at 3413.110 kb on + strand at 3413.110 kb on + strand at 3413.110 kb on + strand at 3413.111 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 3,409,653 - +1.3 3,409,653 - +0.5 3,409,653 - +0.1 3,409,653 - +0.1 3,409,653 - +0.8 3,409,684 - +0.2 3,410,011 - Psest_3239 0.11 -0.9 3,410,011 - Psest_3239 0.11 -1.0 3,410,011 - Psest_3239 0.11 -1.5 3,410,011 - Psest_3239 0.11 -1.0 3,410,011 - Psest_3239 0.11 +0.6 3,410,011 - Psest_3239 0.11 -1.4 3,410,048 - Psest_3239 0.24 -1.1 3,410,360 + +0.7 3,410,360 + -0.1 3,410,360 + +0.3 3,410,360 + +0.5 3,410,360 + -1.6 3,410,361 - -0.4 3,410,361 - -0.0 3,410,361 - -1.2 3,410,361 - -0.7 3,410,361 - -1.3 3,410,369 - -1.5 3,410,402 + +1.1 3,410,402 + -1.4 3,410,439 + -1.5 3,410,440 - +2.0 3,410,625 + +2.8 3,410,625 + -0.1 3,410,633 - Psest_3240 0.10 -1.8 3,410,937 + Psest_3240 0.28 +0.5 3,410,937 + Psest_3240 0.28 +0.2 3,410,937 + Psest_3240 0.28 -0.5 3,410,938 - Psest_3240 0.28 -0.3 3,410,938 - Psest_3240 0.28 +0.8 3,410,938 - Psest_3240 0.28 +0.5 3,411,055 + Psest_3240 0.35 -1.5 3,411,055 + Psest_3240 0.35 -1.3 3,411,153 + Psest_3240 0.40 -1.0 3,411,154 - Psest_3240 0.40 -0.9 3,411,561 + Psest_3240 0.64 +0.5 3,411,562 - Psest_3240 0.64 -0.6 3,411,562 - Psest_3240 0.64 +1.1 3,411,667 + Psest_3240 0.70 -0.2 3,411,667 + Psest_3240 0.70 -1.7 3,411,667 + Psest_3240 0.70 -0.8 3,411,668 - Psest_3240 0.70 +1.4 3,411,668 - Psest_3240 0.70 -1.2 3,411,849 + Psest_3240 0.80 -0.6 3,412,110 + +1.1 3,412,110 + -1.2 3,412,110 + +0.0 3,412,110 + +0.8 3,412,110 + +0.7 3,412,110 + +0.8 3,412,110 + +0.5 3,412,110 + +2.6 3,412,110 + -0.2 3,412,111 - +0.1 3,412,111 - -0.3 3,412,111 - +1.3 3,412,111 - -0.8 3,412,111 - -0.7 3,412,156 + -0.4 3,412,472 + Psest_3241 0.26 +1.5 3,412,472 + Psest_3241 0.26 -1.1 3,412,472 + Psest_3241 0.26 -0.5 3,412,473 - Psest_3241 0.27 +1.6 3,412,473 - Psest_3241 0.27 -0.9 3,412,473 - Psest_3241 0.27 +2.5 3,412,473 - Psest_3241 0.27 -1.9 3,412,473 - Psest_3241 0.27 +0.8 3,412,628 + Psest_3241 0.44 +2.5 3,412,653 - Psest_3241 0.46 -0.5 3,412,676 + Psest_3241 0.49 +2.2 3,412,677 - Psest_3241 0.49 -0.4 3,412,677 - Psest_3241 0.49 -0.2 3,412,677 - Psest_3241 0.49 +1.2 3,412,680 + Psest_3241 0.49 +2.4 3,412,705 + Psest_3241 0.52 +0.6 3,412,715 + Psest_3241 0.53 +0.6 3,412,715 + Psest_3241 0.53 +1.0 3,412,715 + Psest_3241 0.53 -0.0 3,412,715 + Psest_3241 0.53 -0.6 3,412,716 - Psest_3241 0.53 +0.8 3,412,716 - Psest_3241 0.53 -1.1 3,412,732 - Psest_3241 0.55 +0.4 3,412,732 - Psest_3241 0.55 +0.2 3,412,760 + Psest_3241 0.58 -0.4 3,412,760 + Psest_3241 0.58 -0.5 3,412,760 + Psest_3241 0.58 +1.0 3,412,761 - Psest_3241 0.58 +0.6 3,412,761 - Psest_3241 0.58 -0.7 3,412,761 - Psest_3241 0.58 -1.5 3,412,761 - Psest_3241 0.58 +1.4 3,412,761 - Psest_3241 0.58 -0.1 3,412,874 + Psest_3241 0.71 +0.3 3,412,875 - Psest_3241 0.71 -1.9 3,413,110 + -1.7 3,413,110 + -0.1 3,413,110 + +1.1 3,413,111 - -1.5
Or see this region's nucleotide sequence