Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3066 and Psest_3067 are separated by 41 nucleotides Psest_3067 and Psest_3068 are separated by 114 nucleotides Psest_3068 and Psest_3069 are separated by 382 nucleotides
Psest_3066: Psest_3066 - hypothetical protein, at 3,241,921 to 3,242,961
_3066
Psest_3067: Psest_3067 - hypothetical protein, at 3,243,003 to 3,243,713
_3067
Psest_3068: Psest_3068 - hypothetical protein, at 3,243,828 to 3,244,793
_3068
Psest_3069: Psest_3069 - 3-oxoadipate enol-lactonase, at 3,245,176 to 3,245,964
_3069
Position (kb)
3243
3244
3245 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3242.878 kb on - strand at 3242.919 kb on - strand at 3243.033 kb on + strand at 3243.033 kb on + strand at 3243.037 kb on + strand at 3243.038 kb on - strand at 3243.120 kb on - strand, within Psest_3067 at 3243.149 kb on - strand, within Psest_3067 at 3243.174 kb on - strand, within Psest_3067 at 3243.174 kb on - strand, within Psest_3067 at 3243.194 kb on + strand, within Psest_3067 at 3243.217 kb on + strand, within Psest_3067 at 3243.249 kb on + strand, within Psest_3067 at 3243.249 kb on + strand, within Psest_3067 at 3243.250 kb on - strand, within Psest_3067 at 3243.381 kb on + strand, within Psest_3067 at 3243.381 kb on + strand, within Psest_3067 at 3243.381 kb on + strand, within Psest_3067 at 3243.381 kb on + strand, within Psest_3067 at 3243.382 kb on - strand, within Psest_3067 at 3243.382 kb on - strand, within Psest_3067 at 3243.382 kb on - strand, within Psest_3067 at 3243.382 kb on - strand, within Psest_3067 at 3243.429 kb on + strand, within Psest_3067 at 3243.430 kb on - strand, within Psest_3067 at 3243.432 kb on + strand, within Psest_3067 at 3243.598 kb on - strand, within Psest_3067 at 3243.733 kb on + strand at 3243.752 kb on + strand at 3243.756 kb on + strand at 3243.757 kb on - strand at 3243.757 kb on - strand at 3243.854 kb on + strand at 3243.944 kb on - strand, within Psest_3068 at 3244.128 kb on + strand, within Psest_3068 at 3244.160 kb on - strand, within Psest_3068 at 3244.311 kb on - strand, within Psest_3068 at 3244.502 kb on + strand, within Psest_3068 at 3244.502 kb on + strand, within Psest_3068 at 3244.502 kb on + strand, within Psest_3068 at 3244.607 kb on - strand, within Psest_3068 at 3244.607 kb on - strand, within Psest_3068 at 3244.647 kb on - strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.681 kb on + strand, within Psest_3068 at 3244.682 kb on - strand, within Psest_3068 at 3244.771 kb on - strand at 3244.771 kb on - strand at 3244.772 kb on + strand at 3244.772 kb on + strand at 3244.772 kb on + strand at 3244.772 kb on + strand at 3244.772 kb on + strand at 3244.772 kb on + strand at 3244.773 kb on - strand at 3244.773 kb on - strand at 3244.773 kb on - strand at 3244.856 kb on + strand at 3244.856 kb on + strand at 3244.902 kb on + strand at 3244.903 kb on - strand at 3244.903 kb on - strand at 3245.005 kb on - strand at 3245.035 kb on + strand at 3245.035 kb on + strand at 3245.089 kb on - strand at 3245.174 kb on - strand at 3245.208 kb on + strand at 3245.208 kb on + strand at 3245.209 kb on - strand at 3245.209 kb on - strand at 3245.237 kb on - strand at 3245.578 kb on + strand, within Psest_3069 at 3245.630 kb on - strand, within Psest_3069 at 3245.630 kb on - strand, within Psest_3069 at 3245.630 kb on - strand, within Psest_3069 at 3245.762 kb on + strand, within Psest_3069
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 3,242,878 - +2.5 3,242,919 - -1.1 3,243,033 + +0.5 3,243,033 + +0.2 3,243,037 + +0.9 3,243,038 - -2.8 3,243,120 - Psest_3067 0.16 +1.1 3,243,149 - Psest_3067 0.21 +0.3 3,243,174 - Psest_3067 0.24 +1.8 3,243,174 - Psest_3067 0.24 -0.2 3,243,194 + Psest_3067 0.27 +0.3 3,243,217 + Psest_3067 0.30 +0.6 3,243,249 + Psest_3067 0.35 +0.4 3,243,249 + Psest_3067 0.35 +0.6 3,243,250 - Psest_3067 0.35 -0.5 3,243,381 + Psest_3067 0.53 -0.7 3,243,381 + Psest_3067 0.53 -3.0 3,243,381 + Psest_3067 0.53 +1.0 3,243,381 + Psest_3067 0.53 -0.6 3,243,382 - Psest_3067 0.53 +1.0 3,243,382 - Psest_3067 0.53 -0.8 3,243,382 - Psest_3067 0.53 +1.4 3,243,382 - Psest_3067 0.53 +0.1 3,243,429 + Psest_3067 0.60 -0.1 3,243,430 - Psest_3067 0.60 +0.9 3,243,432 + Psest_3067 0.60 +1.6 3,243,598 - Psest_3067 0.84 +0.8 3,243,733 + -1.5 3,243,752 + +1.1 3,243,756 + -2.2 3,243,757 - -0.4 3,243,757 - +1.6 3,243,854 + +2.0 3,243,944 - Psest_3068 0.12 +0.6 3,244,128 + Psest_3068 0.31 -0.2 3,244,160 - Psest_3068 0.34 +1.3 3,244,311 - Psest_3068 0.50 -0.4 3,244,502 + Psest_3068 0.70 +0.4 3,244,502 + Psest_3068 0.70 +1.1 3,244,502 + Psest_3068 0.70 -0.9 3,244,607 - Psest_3068 0.81 +2.0 3,244,607 - Psest_3068 0.81 -1.3 3,244,647 - Psest_3068 0.85 +2.7 3,244,681 + Psest_3068 0.88 -1.2 3,244,681 + Psest_3068 0.88 +1.8 3,244,681 + Psest_3068 0.88 +0.7 3,244,681 + Psest_3068 0.88 +0.2 3,244,681 + Psest_3068 0.88 +0.6 3,244,681 + Psest_3068 0.88 +0.9 3,244,681 + Psest_3068 0.88 +2.2 3,244,681 + Psest_3068 0.88 +2.1 3,244,681 + Psest_3068 0.88 +2.3 3,244,681 + Psest_3068 0.88 +0.5 3,244,681 + Psest_3068 0.88 -0.3 3,244,681 + Psest_3068 0.88 +1.2 3,244,681 + Psest_3068 0.88 +0.5 3,244,681 + Psest_3068 0.88 -0.0 3,244,682 - Psest_3068 0.88 +1.0 3,244,771 - +1.0 3,244,771 - +2.3 3,244,772 + -2.2 3,244,772 + +1.5 3,244,772 + +2.3 3,244,772 + -3.0 3,244,772 + -1.4 3,244,772 + -0.2 3,244,773 - -0.2 3,244,773 - +1.6 3,244,773 - -0.4 3,244,856 + -0.7 3,244,856 + -2.0 3,244,902 + +0.6 3,244,903 - +3.9 3,244,903 - +0.9 3,245,005 - +0.2 3,245,035 + -2.1 3,245,035 + +0.6 3,245,089 - -1.6 3,245,174 - +0.9 3,245,208 + -1.9 3,245,208 + -1.9 3,245,209 - +2.3 3,245,209 - +2.2 3,245,237 - -0.0 3,245,578 + Psest_3069 0.51 -1.3 3,245,630 - Psest_3069 0.58 -1.5 3,245,630 - Psest_3069 0.58 +0.8 3,245,630 - Psest_3069 0.58 +2.0 3,245,762 + Psest_3069 0.74 -1.7
Or see this region's nucleotide sequence