Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0145 and Psest_0146 overlap by 4 nucleotides Psest_0146 and Psest_0147 are separated by 191 nucleotides Psest_0147 and Psest_0148 are separated by 88 nucleotides
Psest_0145: Psest_0145 - A/G-specific adenine glycosylase, at 143,312 to 144,379
_0145
Psest_0146: Psest_0146 - Uncharacterized protein involved in outer membrane biogenesis, at 144,376 to 146,601
_0146
Psest_0147: Psest_0147 - Acetyltransferase, GNAT family., at 146,793 to 147,191
_0147
Psest_0148: Psest_0148 - nitrate transport ATP-binding subunits C and D, at 147,280 to 148,062
_0148
Position (kb)
144
145
146
147 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 143.407 kb on + strand at 143.408 kb on - strand at 143.409 kb on + strand at 143.409 kb on + strand at 143.409 kb on + strand at 143.410 kb on - strand at 143.410 kb on - strand at 143.410 kb on - strand at 143.410 kb on - strand at 143.841 kb on + strand, within Psest_0145 at 143.842 kb on - strand, within Psest_0145 at 143.842 kb on - strand, within Psest_0145 at 143.937 kb on + strand, within Psest_0145 at 143.938 kb on - strand, within Psest_0145 at 143.938 kb on - strand, within Psest_0145 at 144.133 kb on - strand, within Psest_0145 at 144.136 kb on - strand, within Psest_0145 at 144.575 kb on + strand at 144.647 kb on + strand, within Psest_0146 at 144.647 kb on + strand, within Psest_0146 at 145.076 kb on - strand, within Psest_0146 at 145.076 kb on - strand, within Psest_0146 at 145.076 kb on - strand, within Psest_0146 at 145.112 kb on + strand, within Psest_0146 at 145.112 kb on + strand, within Psest_0146 at 145.113 kb on - strand, within Psest_0146 at 145.255 kb on + strand, within Psest_0146 at 146.030 kb on + strand, within Psest_0146 at 146.031 kb on - strand, within Psest_0146 at 146.031 kb on - strand, within Psest_0146 at 146.057 kb on + strand, within Psest_0146 at 146.058 kb on - strand, within Psest_0146 at 146.058 kb on - strand, within Psest_0146 at 146.058 kb on - strand, within Psest_0146 at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.499 kb on - strand at 146.499 kb on - strand at 146.499 kb on - strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.616 kb on - strand at 146.616 kb on - strand at 146.616 kb on - strand at 146.642 kb on + strand at 146.643 kb on - strand at 146.643 kb on - strand at 146.644 kb on + strand at 146.644 kb on + strand at 146.644 kb on + strand at 146.795 kb on - strand at 147.087 kb on - strand, within Psest_0147 at 147.091 kb on + strand, within Psest_0147 at 147.091 kb on + strand, within Psest_0147 at 147.092 kb on - strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.145 kb on - strand, within Psest_0147 at 147.181 kb on + strand at 147.181 kb on + strand at 147.181 kb on + strand at 147.182 kb on - strand at 147.231 kb on + strand at 147.338 kb on + strand at 147.338 kb on + strand at 147.339 kb on - strand at 147.339 kb on - strand at 147.339 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 143,407 + +0.2 143,408 - -0.3 143,409 + -0.2 143,409 + +0.7 143,409 + +0.3 143,410 - -0.1 143,410 - +0.8 143,410 - -1.6 143,410 - +0.6 143,841 + Psest_0145 0.50 -0.5 143,842 - Psest_0145 0.50 -2.1 143,842 - Psest_0145 0.50 -0.5 143,937 + Psest_0145 0.59 +0.4 143,938 - Psest_0145 0.59 +1.2 143,938 - Psest_0145 0.59 -1.9 144,133 - Psest_0145 0.77 +0.6 144,136 - Psest_0145 0.77 +0.2 144,575 + +1.4 144,647 + Psest_0146 0.12 +1.1 144,647 + Psest_0146 0.12 +2.4 145,076 - Psest_0146 0.31 +2.5 145,076 - Psest_0146 0.31 +1.8 145,076 - Psest_0146 0.31 -1.7 145,112 + Psest_0146 0.33 +2.4 145,112 + Psest_0146 0.33 -1.6 145,113 - Psest_0146 0.33 +2.8 145,255 + Psest_0146 0.39 -0.8 146,030 + Psest_0146 0.74 -1.1 146,031 - Psest_0146 0.74 -0.9 146,031 - Psest_0146 0.74 +0.9 146,057 + Psest_0146 0.76 +1.2 146,058 - Psest_0146 0.76 +0.3 146,058 - Psest_0146 0.76 +2.7 146,058 - Psest_0146 0.76 +2.3 146,498 + +1.1 146,498 + -0.1 146,498 + -0.9 146,498 + +0.1 146,498 + -1.1 146,498 + +0.9 146,498 + +0.1 146,499 - +0.9 146,499 - -0.6 146,499 - +1.4 146,615 + +1.2 146,615 + -0.4 146,615 + -0.2 146,615 + +0.5 146,615 + -1.1 146,615 + -1.7 146,615 + +0.5 146,615 + +0.9 146,615 + +0.3 146,616 - +0.7 146,616 - -1.4 146,616 - -1.3 146,642 + -0.5 146,643 - -2.6 146,643 - +2.2 146,644 + -1.1 146,644 + +0.6 146,644 + -0.7 146,795 - +0.9 147,087 - Psest_0147 0.74 +2.1 147,091 + Psest_0147 0.75 +0.2 147,091 + Psest_0147 0.75 -0.9 147,092 - Psest_0147 0.75 +0.3 147,124 + Psest_0147 0.83 -0.7 147,124 + Psest_0147 0.83 -0.2 147,124 + Psest_0147 0.83 +2.1 147,125 - Psest_0147 0.83 -0.8 147,125 - Psest_0147 0.83 +0.2 147,125 - Psest_0147 0.83 +1.0 147,125 - Psest_0147 0.83 +0.5 147,125 - Psest_0147 0.83 +0.6 147,125 - Psest_0147 0.83 -0.2 147,145 - Psest_0147 0.88 +0.5 147,181 + +1.3 147,181 + -1.3 147,181 + +4.1 147,182 - +1.7 147,231 + -2.0 147,338 + +1.5 147,338 + +0.2 147,339 - +1.0 147,339 - +2.8 147,339 - -2.1
Or see this region's nucleotide sequence