Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1203

Experiment: Spectinomycin 0.00625 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1201 and Psest_1202 are separated by 200 nucleotidesPsest_1202 and Psest_1203 are separated by 80 nucleotidesPsest_1203 and Psest_1204 are separated by 347 nucleotides Psest_1201: Psest_1201 - hydroxyisourate hydrolase, at 1,304,453 to 1,304,806 _1201 Psest_1202: Psest_1202 - Putative threonine efflux protein, at 1,305,007 to 1,305,633 _1202 Psest_1203: Psest_1203 - Permeases, at 1,305,714 to 1,307,060 _1203 Psest_1204: Psest_1204 - Transcriptional regulators, at 1,307,408 to 1,308,163 _1204 Position (kb) 1305 1306 1307 1308Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1304.750 kb on + strand, within Psest_1201at 1304.751 kb on - strand, within Psest_1201at 1304.751 kb on - strand, within Psest_1201at 1304.751 kb on - strand, within Psest_1201at 1304.783 kb on + strandat 1304.783 kb on + strandat 1304.784 kb on - strandat 1304.784 kb on - strandat 1304.831 kb on + strandat 1304.831 kb on + strandat 1304.869 kb on + strandat 1304.870 kb on - strandat 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.129 kb on - strand, within Psest_1202at 1305.268 kb on + strand, within Psest_1202at 1305.269 kb on - strand, within Psest_1202at 1305.269 kb on - strand, within Psest_1202at 1305.269 kb on - strand, within Psest_1202at 1305.269 kb on - strand, within Psest_1202at 1305.269 kb on - strand, within Psest_1202at 1305.380 kb on + strand, within Psest_1202at 1305.380 kb on + strand, within Psest_1202at 1305.381 kb on - strand, within Psest_1202at 1305.381 kb on - strand, within Psest_1202at 1305.381 kb on - strand, within Psest_1202at 1305.381 kb on - strand, within Psest_1202at 1305.389 kb on + strand, within Psest_1202at 1305.389 kb on + strand, within Psest_1202at 1305.389 kb on + strand, within Psest_1202at 1305.389 kb on + strand, within Psest_1202at 1305.389 kb on + strand, within Psest_1202at 1305.389 kb on + strand, within Psest_1202at 1305.389 kb on + strand, within Psest_1202at 1305.389 kb on + strand, within Psest_1202at 1305.390 kb on - strand, within Psest_1202at 1305.527 kb on - strand, within Psest_1202at 1305.533 kb on + strand, within Psest_1202at 1305.533 kb on + strand, within Psest_1202at 1305.534 kb on - strand, within Psest_1202at 1305.650 kb on + strandat 1305.757 kb on + strandat 1305.757 kb on + strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.758 kb on - strandat 1305.811 kb on + strandat 1305.812 kb on - strandat 1305.812 kb on - strandat 1305.812 kb on - strandat 1305.812 kb on - strandat 1305.812 kb on - strandat 1305.812 kb on - strandat 1305.812 kb on - strandat 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.952 kb on + strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.953 kb on - strand, within Psest_1203at 1305.976 kb on + strand, within Psest_1203at 1305.976 kb on + strand, within Psest_1203at 1306.096 kb on + strand, within Psest_1203at 1306.096 kb on + strand, within Psest_1203at 1306.096 kb on + strand, within Psest_1203at 1306.096 kb on + strand, within Psest_1203at 1306.097 kb on - strand, within Psest_1203at 1306.097 kb on - strand, within Psest_1203at 1306.295 kb on + strand, within Psest_1203at 1306.366 kb on + strand, within Psest_1203at 1306.367 kb on - strand, within Psest_1203at 1306.434 kb on + strand, within Psest_1203at 1306.434 kb on + strand, within Psest_1203at 1306.435 kb on - strand, within Psest_1203at 1306.435 kb on - strand, within Psest_1203at 1306.435 kb on - strand, within Psest_1203at 1306.707 kb on + strand, within Psest_1203at 1306.707 kb on + strand, within Psest_1203at 1306.742 kb on + strand, within Psest_1203at 1306.742 kb on + strand, within Psest_1203at 1306.743 kb on - strand, within Psest_1203at 1306.743 kb on - strand, within Psest_1203at 1306.743 kb on - strand, within Psest_1203at 1306.743 kb on - strand, within Psest_1203at 1306.743 kb on - strand, within Psest_1203at 1306.743 kb on - strand, within Psest_1203at 1306.743 kb on - strand, within Psest_1203at 1306.743 kb on - strand, within Psest_1203at 1306.745 kb on - strand, within Psest_1203at 1306.745 kb on - strand, within Psest_1203at 1306.745 kb on - strand, within Psest_1203at 1306.745 kb on - strand, within Psest_1203at 1306.745 kb on - strand, within Psest_1203at 1306.802 kb on - strand, within Psest_1203at 1306.802 kb on - strand, within Psest_1203at 1306.912 kb on + strand, within Psest_1203at 1306.913 kb on - strand, within Psest_1203at 1306.913 kb on - strand, within Psest_1203at 1306.913 kb on - strand, within Psest_1203at 1306.913 kb on - strand, within Psest_1203at 1306.913 kb on - strand, within Psest_1203at 1306.913 kb on - strand, within Psest_1203at 1307.103 kb on + strandat 1307.104 kb on - strandat 1307.180 kb on + strandat 1307.192 kb on + strandat 1307.210 kb on + strandat 1307.252 kb on + strandat 1307.253 kb on - strandat 1307.261 kb on + strandat 1307.261 kb on + strandat 1307.262 kb on - strandat 1307.262 kb on - strandat 1307.329 kb on + strandat 1307.486 kb on + strand, within Psest_1204at 1307.565 kb on + strand, within Psest_1204at 1307.565 kb on + strand, within Psest_1204at 1307.566 kb on - strand, within Psest_1204at 1307.629 kb on - strand, within Psest_1204at 1307.973 kb on - strand, within Psest_1204at 1307.973 kb on - strand, within Psest_1204

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml
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1,304,750 + Psest_1201 0.84 +0.1
1,304,751 - Psest_1201 0.84 +1.2
1,304,751 - Psest_1201 0.84 -1.0
1,304,751 - Psest_1201 0.84 -1.2
1,304,783 + -2.4
1,304,783 + -1.3
1,304,784 - -1.3
1,304,784 - -0.4
1,304,831 + -0.9
1,304,831 + -0.2
1,304,869 + -0.3
1,304,870 - +0.7
1,305,073 + Psest_1202 0.11 +1.0
1,305,073 + Psest_1202 0.11 -0.8
1,305,073 + Psest_1202 0.11 -0.8
1,305,073 + Psest_1202 0.11 +0.8
1,305,073 + Psest_1202 0.11 +1.5
1,305,073 + Psest_1202 0.11 -1.9
1,305,073 + Psest_1202 0.11 -0.4
1,305,073 + Psest_1202 0.11 -1.4
1,305,073 + Psest_1202 0.11 +3.2
1,305,073 + Psest_1202 0.11 +1.1
1,305,073 + Psest_1202 0.11 -0.1
1,305,073 + Psest_1202 0.11 -0.1
1,305,073 + Psest_1202 0.11 +2.8
1,305,073 + Psest_1202 0.11 +1.6
1,305,073 + Psest_1202 0.11 +1.6
1,305,073 + Psest_1202 0.11 +1.4
1,305,073 + Psest_1202 0.11 +1.0
1,305,073 + Psest_1202 0.11 -1.3
1,305,073 + Psest_1202 0.11 -2.1
1,305,073 + Psest_1202 0.11 +1.6
1,305,073 + Psest_1202 0.11 -0.4
1,305,074 - Psest_1202 0.11 -1.0
1,305,074 - Psest_1202 0.11 -1.5
1,305,074 - Psest_1202 0.11 +0.8
1,305,074 - Psest_1202 0.11 +0.3
1,305,074 - Psest_1202 0.11 -1.3
1,305,074 - Psest_1202 0.11 -0.4
1,305,074 - Psest_1202 0.11 -1.0
1,305,074 - Psest_1202 0.11 +0.1
1,305,074 - Psest_1202 0.11 -1.3
1,305,074 - Psest_1202 0.11 +0.7
1,305,074 - Psest_1202 0.11 -1.9
1,305,074 - Psest_1202 0.11 +0.8
1,305,074 - Psest_1202 0.11 -0.9
1,305,074 - Psest_1202 0.11 -1.4
1,305,074 - Psest_1202 0.11 -1.1
1,305,074 - Psest_1202 0.11 +0.1
1,305,074 - Psest_1202 0.11 -1.9
1,305,074 - Psest_1202 0.11 +0.2
1,305,074 - Psest_1202 0.11 -0.2
1,305,074 - Psest_1202 0.11 +2.8
1,305,074 - Psest_1202 0.11 -0.8
1,305,074 - Psest_1202 0.11 +0.4
1,305,074 - Psest_1202 0.11 -0.3
1,305,074 - Psest_1202 0.11 -0.0
1,305,074 - Psest_1202 0.11 -0.7
1,305,074 - Psest_1202 0.11 +1.4
1,305,074 - Psest_1202 0.11 +0.4
1,305,074 - Psest_1202 0.11 -1.7
1,305,129 - Psest_1202 0.19 +0.8
1,305,268 + Psest_1202 0.42 -0.1
1,305,269 - Psest_1202 0.42 -0.2
1,305,269 - Psest_1202 0.42 -0.0
1,305,269 - Psest_1202 0.42 -0.0
1,305,269 - Psest_1202 0.42 +0.9
1,305,269 - Psest_1202 0.42 +0.7
1,305,380 + Psest_1202 0.59 +0.3
1,305,380 + Psest_1202 0.59 +0.9
1,305,381 - Psest_1202 0.60 -1.1
1,305,381 - Psest_1202 0.60 +0.5
1,305,381 - Psest_1202 0.60 -0.2
1,305,381 - Psest_1202 0.60 +0.8
1,305,389 + Psest_1202 0.61 +0.3
1,305,389 + Psest_1202 0.61 -1.5
1,305,389 + Psest_1202 0.61 +1.6
1,305,389 + Psest_1202 0.61 -0.6
1,305,389 + Psest_1202 0.61 +0.0
1,305,389 + Psest_1202 0.61 +1.2
1,305,389 + Psest_1202 0.61 -2.4
1,305,389 + Psest_1202 0.61 +2.0
1,305,390 - Psest_1202 0.61 -0.3
1,305,527 - Psest_1202 0.83 -0.7
1,305,533 + Psest_1202 0.84 +0.6
1,305,533 + Psest_1202 0.84 +2.2
1,305,534 - Psest_1202 0.84 -1.3
1,305,650 + -0.9
1,305,757 + -0.6
1,305,757 + -0.8
1,305,758 - +1.4
1,305,758 - -1.8
1,305,758 - -1.3
1,305,758 - +0.9
1,305,758 - +0.4
1,305,758 - -0.1
1,305,758 - -0.5
1,305,758 - -0.3
1,305,758 - -0.6
1,305,758 - +1.3
1,305,758 - +0.7
1,305,758 - +1.2
1,305,811 + -0.7
1,305,812 - +2.4
1,305,812 - +0.1
1,305,812 - +0.2
1,305,812 - +0.7
1,305,812 - -1.2
1,305,812 - +0.0
1,305,812 - +1.2
1,305,952 + Psest_1203 0.18 +1.8
1,305,952 + Psest_1203 0.18 +1.4
1,305,952 + Psest_1203 0.18 +0.1
1,305,952 + Psest_1203 0.18 +0.6
1,305,952 + Psest_1203 0.18 -0.6
1,305,952 + Psest_1203 0.18 -3.7
1,305,952 + Psest_1203 0.18 -1.6
1,305,952 + Psest_1203 0.18 +1.1
1,305,952 + Psest_1203 0.18 +3.3
1,305,952 + Psest_1203 0.18 +0.7
1,305,952 + Psest_1203 0.18 +1.3
1,305,952 + Psest_1203 0.18 -1.3
1,305,952 + Psest_1203 0.18 +0.8
1,305,952 + Psest_1203 0.18 -0.7
1,305,953 - Psest_1203 0.18 +2.0
1,305,953 - Psest_1203 0.18 -1.1
1,305,953 - Psest_1203 0.18 +1.6
1,305,953 - Psest_1203 0.18 -1.3
1,305,953 - Psest_1203 0.18 +0.3
1,305,953 - Psest_1203 0.18 -0.0
1,305,953 - Psest_1203 0.18 -0.8
1,305,953 - Psest_1203 0.18 +1.1
1,305,953 - Psest_1203 0.18 -0.6
1,305,953 - Psest_1203 0.18 -1.1
1,305,953 - Psest_1203 0.18 -0.8
1,305,953 - Psest_1203 0.18 +0.7
1,305,976 + Psest_1203 0.19 -0.6
1,305,976 + Psest_1203 0.19 +1.6
1,306,096 + Psest_1203 0.28 +2.9
1,306,096 + Psest_1203 0.28 -1.1
1,306,096 + Psest_1203 0.28 +1.1
1,306,096 + Psest_1203 0.28 -1.6
1,306,097 - Psest_1203 0.28 +0.4
1,306,097 - Psest_1203 0.28 -1.0
1,306,295 + Psest_1203 0.43 +0.5
1,306,366 + Psest_1203 0.48 -2.6
1,306,367 - Psest_1203 0.48 -0.7
1,306,434 + Psest_1203 0.53 +0.9
1,306,434 + Psest_1203 0.53 -0.5
1,306,435 - Psest_1203 0.54 +1.2
1,306,435 - Psest_1203 0.54 +0.1
1,306,435 - Psest_1203 0.54 +0.7
1,306,707 + Psest_1203 0.74 -0.2
1,306,707 + Psest_1203 0.74 -2.0
1,306,742 + Psest_1203 0.76 +0.9
1,306,742 + Psest_1203 0.76 -0.5
1,306,743 - Psest_1203 0.76 +0.3
1,306,743 - Psest_1203 0.76 +1.0
1,306,743 - Psest_1203 0.76 -1.0
1,306,743 - Psest_1203 0.76 -0.8
1,306,743 - Psest_1203 0.76 +1.1
1,306,743 - Psest_1203 0.76 -1.7
1,306,743 - Psest_1203 0.76 -0.0
1,306,743 - Psest_1203 0.76 -0.7
1,306,745 - Psest_1203 0.77 -3.3
1,306,745 - Psest_1203 0.77 -0.6
1,306,745 - Psest_1203 0.77 -0.4
1,306,745 - Psest_1203 0.77 -0.3
1,306,745 - Psest_1203 0.77 +1.5
1,306,802 - Psest_1203 0.81 +0.9
1,306,802 - Psest_1203 0.81 -0.5
1,306,912 + Psest_1203 0.89 +2.5
1,306,913 - Psest_1203 0.89 -0.5
1,306,913 - Psest_1203 0.89 -1.4
1,306,913 - Psest_1203 0.89 +0.8
1,306,913 - Psest_1203 0.89 -1.6
1,306,913 - Psest_1203 0.89 +1.6
1,306,913 - Psest_1203 0.89 -0.7
1,307,103 + +0.5
1,307,104 - -3.5
1,307,180 + -2.1
1,307,192 + -1.6
1,307,210 + -1.7
1,307,252 + -0.1
1,307,253 - -1.2
1,307,261 + -2.2
1,307,261 + +0.3
1,307,262 - +0.6
1,307,262 - -1.0
1,307,329 + -1.6
1,307,486 + Psest_1204 0.10 -0.4
1,307,565 + Psest_1204 0.21 -3.0
1,307,565 + Psest_1204 0.21 -0.6
1,307,566 - Psest_1204 0.21 -2.0
1,307,629 - Psest_1204 0.29 +0.3
1,307,973 - Psest_1204 0.75 -3.4
1,307,973 - Psest_1204 0.75 -2.0

Or see this region's nucleotide sequence