Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1200

Experiment: Spectinomycin 0.00625 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1198 and Psest_1199 are separated by 60 nucleotidesPsest_1199 and Psest_1200 overlap by 4 nucleotidesPsest_1200 and Psest_1201 are separated by 280 nucleotidesPsest_1201 and Psest_1202 are separated by 200 nucleotides Psest_1198: Psest_1198 - allantoicase, at 1,301,672 to 1,302,667 _1198 Psest_1199: Psest_1199 - OHCU decarboxylase, at 1,302,728 to 1,303,243 _1199 Psest_1200: Psest_1200 - putative urate catabolism protein, at 1,303,240 to 1,304,172 _1200 Psest_1201: Psest_1201 - hydroxyisourate hydrolase, at 1,304,453 to 1,304,806 _1201 Psest_1202: Psest_1202 - Putative threonine efflux protein, at 1,305,007 to 1,305,633 _1202 Position (kb) 1303 1304 1305Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1302.348 kb on + strand, within Psest_1198at 1302.348 kb on + strand, within Psest_1198at 1302.348 kb on + strand, within Psest_1198at 1302.349 kb on - strand, within Psest_1198at 1302.349 kb on - strand, within Psest_1198at 1302.349 kb on - strand, within Psest_1198at 1302.349 kb on - strand, within Psest_1198at 1302.349 kb on - strand, within Psest_1198at 1302.349 kb on - strand, within Psest_1198at 1302.460 kb on - strand, within Psest_1198at 1302.636 kb on + strandat 1302.700 kb on + strandat 1302.700 kb on + strandat 1302.700 kb on + strandat 1302.701 kb on - strandat 1302.701 kb on - strandat 1302.701 kb on - strandat 1302.729 kb on + strandat 1302.729 kb on + strandat 1302.729 kb on + strandat 1302.729 kb on + strandat 1302.729 kb on + strandat 1302.729 kb on + strandat 1302.730 kb on - strandat 1302.894 kb on + strand, within Psest_1199at 1302.895 kb on - strand, within Psest_1199at 1302.895 kb on - strand, within Psest_1199at 1302.895 kb on - strand, within Psest_1199at 1302.895 kb on - strand, within Psest_1199at 1303.107 kb on + strand, within Psest_1199at 1303.170 kb on + strand, within Psest_1199at 1303.170 kb on + strand, within Psest_1199at 1303.170 kb on + strand, within Psest_1199at 1303.443 kb on - strand, within Psest_1200at 1303.469 kb on + strand, within Psest_1200at 1303.470 kb on - strand, within Psest_1200at 1303.541 kb on + strand, within Psest_1200at 1303.541 kb on + strand, within Psest_1200at 1303.607 kb on + strand, within Psest_1200at 1303.607 kb on + strand, within Psest_1200at 1303.608 kb on - strand, within Psest_1200at 1303.608 kb on - strand, within Psest_1200at 1303.623 kb on - strand, within Psest_1200at 1303.682 kb on + strand, within Psest_1200at 1303.683 kb on - strand, within Psest_1200at 1303.683 kb on - strand, within Psest_1200at 1303.683 kb on - strand, within Psest_1200at 1303.949 kb on + strand, within Psest_1200at 1303.949 kb on + strand, within Psest_1200at 1303.949 kb on + strand, within Psest_1200at 1303.949 kb on + strand, within Psest_1200at 1303.949 kb on + strand, within Psest_1200at 1304.069 kb on + strand, within Psest_1200at 1304.070 kb on - strand, within Psest_1200at 1304.159 kb on + strandat 1304.188 kb on - strandat 1304.249 kb on + strandat 1304.262 kb on - strandat 1304.270 kb on + strandat 1304.271 kb on - strandat 1304.321 kb on + strandat 1304.476 kb on - strandat 1304.476 kb on - strandat 1304.526 kb on - strand, within Psest_1201at 1304.618 kb on + strand, within Psest_1201at 1304.633 kb on + strand, within Psest_1201at 1304.634 kb on - strand, within Psest_1201at 1304.660 kb on + strand, within Psest_1201at 1304.661 kb on - strand, within Psest_1201at 1304.668 kb on + strand, within Psest_1201at 1304.668 kb on + strand, within Psest_1201at 1304.750 kb on + strand, within Psest_1201at 1304.751 kb on - strand, within Psest_1201at 1304.751 kb on - strand, within Psest_1201at 1304.751 kb on - strand, within Psest_1201at 1304.783 kb on + strandat 1304.783 kb on + strandat 1304.784 kb on - strandat 1304.784 kb on - strandat 1304.831 kb on + strandat 1304.831 kb on + strandat 1304.869 kb on + strandat 1304.870 kb on - strandat 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.073 kb on + strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.074 kb on - strand, within Psest_1202at 1305.129 kb on - strand, within Psest_1202

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml
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1,302,348 + Psest_1198 0.68 +1.8
1,302,348 + Psest_1198 0.68 +0.8
1,302,348 + Psest_1198 0.68 +0.7
1,302,349 - Psest_1198 0.68 -0.6
1,302,349 - Psest_1198 0.68 +2.1
1,302,349 - Psest_1198 0.68 -0.5
1,302,349 - Psest_1198 0.68 +0.8
1,302,349 - Psest_1198 0.68 +0.4
1,302,349 - Psest_1198 0.68 +0.4
1,302,460 - Psest_1198 0.79 +1.8
1,302,636 + -1.3
1,302,700 + -1.1
1,302,700 + +0.1
1,302,700 + +0.5
1,302,701 - +0.2
1,302,701 - -1.6
1,302,701 - -0.8
1,302,729 + +1.2
1,302,729 + +0.4
1,302,729 + -1.1
1,302,729 + +1.4
1,302,729 + -1.0
1,302,729 + +1.2
1,302,730 - +0.2
1,302,894 + Psest_1199 0.32 -1.4
1,302,895 - Psest_1199 0.32 -0.6
1,302,895 - Psest_1199 0.32 +0.5
1,302,895 - Psest_1199 0.32 -0.8
1,302,895 - Psest_1199 0.32 +0.8
1,303,107 + Psest_1199 0.73 +1.3
1,303,170 + Psest_1199 0.86 -0.0
1,303,170 + Psest_1199 0.86 -0.5
1,303,170 + Psest_1199 0.86 +0.9
1,303,443 - Psest_1200 0.22 -1.9
1,303,469 + Psest_1200 0.25 -0.6
1,303,470 - Psest_1200 0.25 +1.5
1,303,541 + Psest_1200 0.32 +0.1
1,303,541 + Psest_1200 0.32 +0.2
1,303,607 + Psest_1200 0.39 +2.2
1,303,607 + Psest_1200 0.39 -0.2
1,303,608 - Psest_1200 0.39 -0.8
1,303,608 - Psest_1200 0.39 -1.0
1,303,623 - Psest_1200 0.41 -0.8
1,303,682 + Psest_1200 0.47 +1.1
1,303,683 - Psest_1200 0.47 -1.9
1,303,683 - Psest_1200 0.47 -1.1
1,303,683 - Psest_1200 0.47 -0.8
1,303,949 + Psest_1200 0.76 -0.4
1,303,949 + Psest_1200 0.76 -1.6
1,303,949 + Psest_1200 0.76 -0.7
1,303,949 + Psest_1200 0.76 -0.2
1,303,949 + Psest_1200 0.76 +1.6
1,304,069 + Psest_1200 0.89 +0.4
1,304,070 - Psest_1200 0.89 -0.8
1,304,159 + -0.2
1,304,188 - -0.8
1,304,249 + -1.5
1,304,262 - -0.1
1,304,270 + -1.4
1,304,271 - -0.8
1,304,321 + -2.4
1,304,476 - +0.1
1,304,476 - +1.0
1,304,526 - Psest_1201 0.21 +0.8
1,304,618 + Psest_1201 0.47 +3.0
1,304,633 + Psest_1201 0.51 -2.5
1,304,634 - Psest_1201 0.51 +1.1
1,304,660 + Psest_1201 0.58 +0.3
1,304,661 - Psest_1201 0.59 +0.3
1,304,668 + Psest_1201 0.61 +1.3
1,304,668 + Psest_1201 0.61 +0.7
1,304,750 + Psest_1201 0.84 +0.1
1,304,751 - Psest_1201 0.84 +1.2
1,304,751 - Psest_1201 0.84 -1.0
1,304,751 - Psest_1201 0.84 -1.2
1,304,783 + -2.4
1,304,783 + -1.3
1,304,784 - -1.3
1,304,784 - -0.4
1,304,831 + -0.9
1,304,831 + -0.2
1,304,869 + -0.3
1,304,870 - +0.7
1,305,073 + Psest_1202 0.11 +1.0
1,305,073 + Psest_1202 0.11 -0.8
1,305,073 + Psest_1202 0.11 -0.8
1,305,073 + Psest_1202 0.11 +0.8
1,305,073 + Psest_1202 0.11 +1.5
1,305,073 + Psest_1202 0.11 -1.9
1,305,073 + Psest_1202 0.11 -0.4
1,305,073 + Psest_1202 0.11 -1.4
1,305,073 + Psest_1202 0.11 +3.2
1,305,073 + Psest_1202 0.11 +1.1
1,305,073 + Psest_1202 0.11 -0.1
1,305,073 + Psest_1202 0.11 -0.1
1,305,073 + Psest_1202 0.11 +2.8
1,305,073 + Psest_1202 0.11 +1.6
1,305,073 + Psest_1202 0.11 +1.6
1,305,073 + Psest_1202 0.11 +1.4
1,305,073 + Psest_1202 0.11 +1.0
1,305,073 + Psest_1202 0.11 -1.3
1,305,073 + Psest_1202 0.11 -2.1
1,305,073 + Psest_1202 0.11 +1.6
1,305,073 + Psest_1202 0.11 -0.4
1,305,074 - Psest_1202 0.11 -1.0
1,305,074 - Psest_1202 0.11 -1.5
1,305,074 - Psest_1202 0.11 +0.8
1,305,074 - Psest_1202 0.11 +0.3
1,305,074 - Psest_1202 0.11 -1.3
1,305,074 - Psest_1202 0.11 -0.4
1,305,074 - Psest_1202 0.11 -1.0
1,305,074 - Psest_1202 0.11 +0.1
1,305,074 - Psest_1202 0.11 -1.3
1,305,074 - Psest_1202 0.11 +0.7
1,305,074 - Psest_1202 0.11 -1.9
1,305,074 - Psest_1202 0.11 +0.8
1,305,074 - Psest_1202 0.11 -0.9
1,305,074 - Psest_1202 0.11 -1.4
1,305,074 - Psest_1202 0.11 -1.1
1,305,074 - Psest_1202 0.11 +0.1
1,305,074 - Psest_1202 0.11 -1.9
1,305,074 - Psest_1202 0.11 +0.2
1,305,074 - Psest_1202 0.11 -0.2
1,305,074 - Psest_1202 0.11 +2.8
1,305,074 - Psest_1202 0.11 -0.8
1,305,074 - Psest_1202 0.11 +0.4
1,305,074 - Psest_1202 0.11 -0.3
1,305,074 - Psest_1202 0.11 -0.0
1,305,074 - Psest_1202 0.11 -0.7
1,305,074 - Psest_1202 0.11 +1.4
1,305,074 - Psest_1202 0.11 +0.4
1,305,074 - Psest_1202 0.11 -1.7
1,305,129 - Psest_1202 0.19 +0.8

Or see this region's nucleotide sequence