Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2303 and Psest_2304 are separated by 140 nucleotides Psest_2304 and Psest_2305 are separated by 6 nucleotides Psest_2305 and Psest_2306 overlap by 4 nucleotides
Psest_2303: Psest_2303 - glutaminyl-tRNA synthetase, at 2,453,444 to 2,455,174
_2303
Psest_2304: Psest_2304 - Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family, at 2,455,315 to 2,455,809
_2304
Psest_2305: Psest_2305 - UDP-2,3-diacylglucosamine hydrolase, at 2,455,816 to 2,456,538
_2305
Psest_2306: Psest_2306 - Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA, at 2,456,535 to 2,457,185
_2306
Position (kb)
2455
2456 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2455.402 kb on + strand, within Psest_2304 at 2456.536 kb on + strand at 2456.537 kb on - strand at 2456.659 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.675 kb on - strand, within Psest_2306 at 2456.717 kb on - strand, within Psest_2306 at 2456.727 kb on + strand, within Psest_2306 at 2456.728 kb on - strand, within Psest_2306 at 2456.728 kb on - strand, within Psest_2306
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 2,455,402 + Psest_2304 0.18 -2.0 2,456,536 + -0.9 2,456,537 - +0.9 2,456,659 + Psest_2306 0.19 -2.0 2,456,674 + Psest_2306 0.21 +1.4 2,456,674 + Psest_2306 0.21 -1.1 2,456,674 + Psest_2306 0.21 +0.5 2,456,675 - Psest_2306 0.22 +2.5 2,456,717 - Psest_2306 0.28 +1.9 2,456,727 + Psest_2306 0.29 +0.6 2,456,728 - Psest_2306 0.30 -0.4 2,456,728 - Psest_2306 0.30 -1.8
Or see this region's nucleotide sequence