Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1985 and Psest_1986 overlap by 4 nucleotides Psest_1986 and Psest_1987 overlap by 4 nucleotides Psest_1987 and Psest_1988 are separated by 191 nucleotides
Psest_1985: Psest_1985 - histidinol-phosphate aminotransferase, at 2,101,462 to 2,102,571
_1985
Psest_1986: Psest_1986 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 2,102,568 to 2,104,811
_1986
Psest_1987: Psest_1987 - cytidylate kinase, at 2,104,808 to 2,105,497
_1987
Psest_1988: Psest_1988 - ribosomal protein S1, at 2,105,689 to 2,107,371
_1988
Position (kb)
2102
2103
2104
2105 Strain fitness (log2 ratio)
-1
0
1
2 at 2101.625 kb on + strand, within Psest_1985 at 2101.625 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.059 kb on + strand, within Psest_1985 at 2102.182 kb on + strand, within Psest_1985 at 2102.213 kb on + strand, within Psest_1985 at 2102.247 kb on + strand, within Psest_1985 at 2102.293 kb on + strand, within Psest_1985 at 2103.460 kb on + strand, within Psest_1986 at 2103.477 kb on + strand, within Psest_1986
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 2,101,625 + Psest_1985 0.15 -1.2 2,101,625 + Psest_1985 0.15 -1.6 2,102,059 + Psest_1985 0.54 +1.9 2,102,059 + Psest_1985 0.54 -0.4 2,102,059 + Psest_1985 0.54 -1.2 2,102,059 + Psest_1985 0.54 +0.2 2,102,059 + Psest_1985 0.54 -1.1 2,102,059 + Psest_1985 0.54 -1.6 2,102,059 + Psest_1985 0.54 +0.5 2,102,182 + Psest_1985 0.65 +0.1 2,102,213 + Psest_1985 0.68 -1.4 2,102,247 + Psest_1985 0.71 -0.1 2,102,293 + Psest_1985 0.75 -0.0 2,103,460 + Psest_1986 0.40 -1.4 2,103,477 + Psest_1986 0.41 -0.8
Or see this region's nucleotide sequence