Experiment: Benzethonium chloride 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2536 and Psest_2537 are separated by 205 nucleotides Psest_2537 and Psest_2538 are separated by 106 nucleotides Psest_2538 and Psest_2539 are separated by 24 nucleotides
Psest_2536: Psest_2536 - NAD+ synthetase, at 2,716,576 to 2,717,403
_2536
Psest_2537: Psest_2537 - oxygen-independent coproporphyrinogen III oxidase, at 2,717,609 to 2,718,991
_2537
Psest_2538: Psest_2538 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 2,719,098 to 2,719,832
_2538
Psest_2539: Psest_2539 - adenine phosphoribosyltransferase, at 2,719,857 to 2,720,405
_2539
Position (kb)
2717
2718
2719 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2717.423 kb on - strand at 2717.573 kb on - strand at 2717.674 kb on + strand at 2717.674 kb on + strand at 2717.674 kb on + strand at 2717.674 kb on + strand at 2717.674 kb on + strand at 2717.674 kb on + strand at 2717.674 kb on + strand at 2717.675 kb on - strand at 2717.675 kb on - strand at 2717.675 kb on - strand at 2717.777 kb on + strand, within Psest_2537 at 2717.810 kb on + strand, within Psest_2537 at 2717.814 kb on - strand, within Psest_2537 at 2717.938 kb on - strand, within Psest_2537 at 2717.938 kb on - strand, within Psest_2537 at 2718.153 kb on + strand, within Psest_2537 at 2718.154 kb on - strand, within Psest_2537 at 2718.154 kb on - strand, within Psest_2537 at 2718.254 kb on + strand, within Psest_2537 at 2718.254 kb on + strand, within Psest_2537 at 2718.254 kb on + strand, within Psest_2537 at 2718.254 kb on + strand, within Psest_2537 at 2718.254 kb on + strand, within Psest_2537 at 2718.255 kb on - strand, within Psest_2537 at 2718.255 kb on - strand, within Psest_2537 at 2718.255 kb on - strand, within Psest_2537 at 2718.255 kb on - strand, within Psest_2537 at 2718.255 kb on - strand, within Psest_2537 at 2718.255 kb on - strand, within Psest_2537 at 2718.255 kb on - strand, within Psest_2537 at 2718.255 kb on - strand, within Psest_2537 at 2718.255 kb on - strand, within Psest_2537 at 2718.255 kb on - strand, within Psest_2537 at 2718.345 kb on - strand, within Psest_2537 at 2718.345 kb on - strand, within Psest_2537 at 2718.345 kb on - strand, within Psest_2537 at 2718.384 kb on + strand, within Psest_2537 at 2718.385 kb on - strand, within Psest_2537 at 2718.385 kb on - strand, within Psest_2537 at 2718.421 kb on - strand, within Psest_2537 at 2718.421 kb on - strand, within Psest_2537 at 2718.421 kb on - strand, within Psest_2537 at 2718.470 kb on + strand, within Psest_2537 at 2718.626 kb on + strand, within Psest_2537 at 2718.648 kb on + strand, within Psest_2537 at 2718.816 kb on - strand, within Psest_2537 at 2718.816 kb on - strand, within Psest_2537 at 2718.947 kb on + strand at 2718.948 kb on - strand at 2718.962 kb on - strand at 2718.989 kb on + strand at 2719.028 kb on - strand at 2719.058 kb on + strand at 2719.072 kb on - strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.097 kb on + strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.098 kb on - strand at 2719.256 kb on - strand, within Psest_2538 at 2719.313 kb on + strand, within Psest_2538 at 2719.774 kb on + strand at 2719.775 kb on - strand at 2719.892 kb on - strand at 2719.940 kb on - strand, within Psest_2539
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.01 mM remove 2,717,423 - +0.0 2,717,573 - +1.8 2,717,674 + -0.2 2,717,674 + +1.6 2,717,674 + -0.8 2,717,674 + -0.5 2,717,674 + +1.1 2,717,674 + +2.2 2,717,674 + -1.6 2,717,675 - -0.3 2,717,675 - -0.3 2,717,675 - +0.6 2,717,777 + Psest_2537 0.12 +3.3 2,717,810 + Psest_2537 0.15 -0.1 2,717,814 - Psest_2537 0.15 -0.1 2,717,938 - Psest_2537 0.24 -0.1 2,717,938 - Psest_2537 0.24 +0.8 2,718,153 + Psest_2537 0.39 -0.4 2,718,154 - Psest_2537 0.39 +1.6 2,718,154 - Psest_2537 0.39 +0.7 2,718,254 + Psest_2537 0.47 +2.4 2,718,254 + Psest_2537 0.47 -0.6 2,718,254 + Psest_2537 0.47 +1.9 2,718,254 + Psest_2537 0.47 +1.8 2,718,254 + Psest_2537 0.47 -0.9 2,718,255 - Psest_2537 0.47 +2.4 2,718,255 - Psest_2537 0.47 +1.5 2,718,255 - Psest_2537 0.47 -1.2 2,718,255 - Psest_2537 0.47 +1.4 2,718,255 - Psest_2537 0.47 -0.5 2,718,255 - Psest_2537 0.47 -2.1 2,718,255 - Psest_2537 0.47 -0.7 2,718,255 - Psest_2537 0.47 -0.6 2,718,255 - Psest_2537 0.47 -0.5 2,718,255 - Psest_2537 0.47 +1.4 2,718,345 - Psest_2537 0.53 -1.8 2,718,345 - Psest_2537 0.53 +0.6 2,718,345 - Psest_2537 0.53 -0.2 2,718,384 + Psest_2537 0.56 -1.6 2,718,385 - Psest_2537 0.56 +1.9 2,718,385 - Psest_2537 0.56 +2.4 2,718,421 - Psest_2537 0.59 +0.1 2,718,421 - Psest_2537 0.59 +0.6 2,718,421 - Psest_2537 0.59 +1.2 2,718,470 + Psest_2537 0.62 +2.8 2,718,626 + Psest_2537 0.74 -1.9 2,718,648 + Psest_2537 0.75 -1.7 2,718,816 - Psest_2537 0.87 +0.4 2,718,816 - Psest_2537 0.87 -0.8 2,718,947 + +0.1 2,718,948 - +0.3 2,718,962 - +0.1 2,718,989 + -1.2 2,719,028 - -0.7 2,719,058 + +0.0 2,719,072 - -1.2 2,719,097 + -0.5 2,719,097 + +0.2 2,719,097 + +0.3 2,719,097 + -2.4 2,719,097 + +2.2 2,719,097 + -1.6 2,719,097 + +1.1 2,719,098 - -0.6 2,719,098 - -0.5 2,719,098 - +0.1 2,719,098 - -0.3 2,719,098 - +3.3 2,719,098 - -0.7 2,719,098 - +1.5 2,719,256 - Psest_2538 0.21 +1.4 2,719,313 + Psest_2538 0.29 -2.5 2,719,774 + -2.5 2,719,775 - -2.1 2,719,892 - -0.6 2,719,940 - Psest_2539 0.15 -0.5
Or see this region's nucleotide sequence