Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0096 and Psest_0097 are separated by 11 nucleotides Psest_0097 and Psest_0098 are separated by 14 nucleotides Psest_0098 and Psest_0099 are separated by 62 nucleotides
Psest_0096: Psest_0096 - phosphonate C-P lyase system protein PhnG, at 96,195 to 96,641
_0096
Psest_0097: Psest_0097 - phosphonates metabolism transcriptional regulator PhnF, at 96,653 to 97,381
_0097
Psest_0098: Psest_0098 - phosphonate ABC transporter, permease protein PhnE, at 97,396 to 98,190
_0098
Psest_0099: Psest_0099 - phosphonate ABC transporter, periplasmic phosphonate binding protein, at 98,253 to 99,257
_0099
Position (kb) 97
98
99 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 96.535 kb on + strand, within Psest_0096 at 96.669 kb on + strand at 96.865 kb on - strand, within Psest_0097 at 97.101 kb on + strand, within Psest_0097 at 97.125 kb on + strand, within Psest_0097 at 97.349 kb on - strand at 97.567 kb on + strand, within Psest_0098 at 97.567 kb on + strand, within Psest_0098 at 97.567 kb on + strand, within Psest_0098 at 97.567 kb on + strand, within Psest_0098 at 97.567 kb on + strand, within Psest_0098 at 97.567 kb on + strand, within Psest_0098 at 97.567 kb on + strand, within Psest_0098 at 97.568 kb on - strand, within Psest_0098 at 97.568 kb on - strand, within Psest_0098 at 97.568 kb on - strand, within Psest_0098 at 97.568 kb on - strand, within Psest_0098 at 97.615 kb on + strand, within Psest_0098 at 97.615 kb on + strand, within Psest_0098 at 97.615 kb on + strand, within Psest_0098 at 97.616 kb on - strand, within Psest_0098 at 97.865 kb on + strand, within Psest_0098 at 97.866 kb on - strand, within Psest_0098 at 97.982 kb on + strand, within Psest_0098 at 97.982 kb on + strand, within Psest_0098 at 97.982 kb on + strand, within Psest_0098 at 97.983 kb on - strand, within Psest_0098 at 97.983 kb on - strand, within Psest_0098 at 97.983 kb on - strand, within Psest_0098 at 97.983 kb on - strand, within Psest_0098 at 98.172 kb on + strand at 98.172 kb on + strand at 98.172 kb on + strand at 98.172 kb on + strand at 98.173 kb on - strand at 98.506 kb on + strand, within Psest_0099 at 98.507 kb on - strand, within Psest_0099 at 98.507 kb on - strand, within Psest_0099 at 98.782 kb on + strand, within Psest_0099 at 98.782 kb on + strand, within Psest_0099 at 98.782 kb on + strand, within Psest_0099 at 98.782 kb on + strand, within Psest_0099 at 98.782 kb on + strand, within Psest_0099 at 98.782 kb on + strand, within Psest_0099 at 98.783 kb on - strand, within Psest_0099 at 98.783 kb on - strand, within Psest_0099 at 98.783 kb on - strand, within Psest_0099 at 98.783 kb on - strand, within Psest_0099 at 98.783 kb on - strand, within Psest_0099 at 98.783 kb on - strand, within Psest_0099 at 98.783 kb on - strand, within Psest_0099 at 98.911 kb on + strand, within Psest_0099 at 98.911 kb on + strand, within Psest_0099 at 98.911 kb on + strand, within Psest_0099 at 98.911 kb on + strand, within Psest_0099 at 98.912 kb on - strand, within Psest_0099 at 98.912 kb on - strand, within Psest_0099 at 98.912 kb on - strand, within Psest_0099 at 98.912 kb on - strand, within Psest_0099 at 98.914 kb on + strand, within Psest_0099 at 98.915 kb on - strand, within Psest_0099 at 98.915 kb on - strand, within Psest_0099 at 99.042 kb on + strand, within Psest_0099
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 96,535 + Psest_0096 0.76 -1.3 96,669 + -3.0 96,865 - Psest_0097 0.29 +0.9 97,101 + Psest_0097 0.61 -0.4 97,125 + Psest_0097 0.65 +1.3 97,349 - -1.4 97,567 + Psest_0098 0.22 +1.8 97,567 + Psest_0098 0.22 +3.5 97,567 + Psest_0098 0.22 -1.8 97,567 + Psest_0098 0.22 +1.8 97,567 + Psest_0098 0.22 -0.0 97,567 + Psest_0098 0.22 +2.3 97,567 + Psest_0098 0.22 -1.7 97,568 - Psest_0098 0.22 -1.8 97,568 - Psest_0098 0.22 +0.2 97,568 - Psest_0098 0.22 +4.9 97,568 - Psest_0098 0.22 -1.7 97,615 + Psest_0098 0.28 -1.2 97,615 + Psest_0098 0.28 +0.0 97,615 + Psest_0098 0.28 +1.6 97,616 - Psest_0098 0.28 -0.8 97,865 + Psest_0098 0.59 +0.8 97,866 - Psest_0098 0.59 +2.2 97,982 + Psest_0098 0.74 +2.5 97,982 + Psest_0098 0.74 +1.2 97,982 + Psest_0098 0.74 +0.1 97,983 - Psest_0098 0.74 +0.8 97,983 - Psest_0098 0.74 +1.8 97,983 - Psest_0098 0.74 -3.2 97,983 - Psest_0098 0.74 -2.1 98,172 + +2.7 98,172 + -1.8 98,172 + +2.2 98,172 + +2.0 98,173 - -2.2 98,506 + Psest_0099 0.25 +1.4 98,507 - Psest_0099 0.25 +0.2 98,507 - Psest_0099 0.25 -0.4 98,782 + Psest_0099 0.53 +0.2 98,782 + Psest_0099 0.53 -2.6 98,782 + Psest_0099 0.53 +0.7 98,782 + Psest_0099 0.53 +0.4 98,782 + Psest_0099 0.53 -2.4 98,782 + Psest_0099 0.53 -1.8 98,783 - Psest_0099 0.53 -2.6 98,783 - Psest_0099 0.53 +0.1 98,783 - Psest_0099 0.53 -2.8 98,783 - Psest_0099 0.53 +1.2 98,783 - Psest_0099 0.53 +3.5 98,783 - Psest_0099 0.53 +2.0 98,783 - Psest_0099 0.53 -1.7 98,911 + Psest_0099 0.65 -0.3 98,911 + Psest_0099 0.65 -2.6 98,911 + Psest_0099 0.65 +1.9 98,911 + Psest_0099 0.65 +5.8 98,912 - Psest_0099 0.66 +1.8 98,912 - Psest_0099 0.66 -0.8 98,912 - Psest_0099 0.66 +0.8 98,912 - Psest_0099 0.66 +1.4 98,914 + Psest_0099 0.66 -1.6 98,915 - Psest_0099 0.66 -3.6 98,915 - Psest_0099 0.66 +2.5 99,042 + Psest_0099 0.79 -1.6
Or see this region's nucleotide sequence