Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0922 and Psest_0923 are separated by 38 nucleotides Psest_0923 and Psest_0925 are separated by 561 nucleotides Psest_0925 and Psest_0926 are separated by 73 nucleotides
Psest_0922: Psest_0922 - alkylhydroperoxidase AhpD family core domain, at 1,002,285 to 1,002,782
_0922
Psest_0923: Psest_0923 - Glutaredoxin, GrxC family, at 1,002,821 to 1,003,093
_0923
Psest_0925: Psest_0925 - Uncharacterized conserved protein, at 1,003,655 to 1,003,945
_0925
Psest_0926: Psest_0926 - Transcriptional regulator, at 1,004,019 to 1,004,945
_0926
Position (kb)
1003
1004 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1002.708 kb on - strand, within Psest_0922 at 1002.752 kb on - strand at 1002.752 kb on - strand at 1002.752 kb on - strand at 1002.752 kb on - strand at 1002.804 kb on + strand at 1002.804 kb on + strand at 1002.804 kb on + strand at 1002.805 kb on - strand at 1002.805 kb on - strand at 1002.814 kb on + strand at 1002.814 kb on + strand at 1002.814 kb on + strand at 1002.814 kb on + strand at 1002.815 kb on - strand at 1002.815 kb on - strand at 1002.815 kb on - strand at 1002.836 kb on + strand at 1002.836 kb on + strand at 1002.836 kb on + strand at 1002.836 kb on + strand at 1002.836 kb on + strand at 1002.837 kb on - strand at 1002.837 kb on - strand at 1002.837 kb on - strand at 1002.837 kb on - strand at 1002.839 kb on - strand at 1002.839 kb on - strand at 1002.839 kb on - strand at 1002.839 kb on - strand at 1002.839 kb on - strand at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.986 kb on + strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1002.987 kb on - strand, within Psest_0923 at 1003.051 kb on + strand, within Psest_0923 at 1003.089 kb on + strand at 1003.089 kb on + strand at 1003.089 kb on + strand at 1003.089 kb on + strand at 1003.090 kb on - strand at 1003.140 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.169 kb on + strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.170 kb on - strand at 1003.198 kb on + strand at 1003.199 kb on - strand at 1003.424 kb on + strand at 1003.471 kb on + strand at 1003.517 kb on + strand at 1003.581 kb on + strand at 1003.582 kb on - strand at 1003.582 kb on - strand at 1003.640 kb on + strand at 1003.640 kb on + strand at 1003.646 kb on + strand at 1003.688 kb on - strand, within Psest_0925 at 1003.693 kb on - strand, within Psest_0925 at 1003.693 kb on - strand, within Psest_0925 at 1003.775 kb on + strand, within Psest_0925 at 1003.776 kb on - strand, within Psest_0925 at 1003.776 kb on - strand, within Psest_0925 at 1003.776 kb on - strand, within Psest_0925 at 1003.848 kb on + strand, within Psest_0925 at 1003.849 kb on - strand, within Psest_0925 at 1004.003 kb on + strand at 1004.003 kb on + strand at 1004.003 kb on + strand at 1004.004 kb on - strand at 1004.007 kb on + strand at 1004.007 kb on + strand at 1004.007 kb on + strand at 1004.007 kb on + strand at 1004.008 kb on - strand at 1004.008 kb on - strand at 1004.008 kb on - strand at 1004.008 kb on - strand at 1004.008 kb on - strand at 1004.008 kb on - strand at 1004.122 kb on + strand, within Psest_0926 at 1004.123 kb on - strand, within Psest_0926 at 1004.150 kb on + strand, within Psest_0926 at 1004.150 kb on + strand, within Psest_0926 at 1004.151 kb on - strand, within Psest_0926 at 1004.220 kb on + strand, within Psest_0926 at 1004.220 kb on + strand, within Psest_0926 at 1004.221 kb on - strand, within Psest_0926 at 1004.221 kb on - strand, within Psest_0926 at 1004.221 kb on - strand, within Psest_0926 at 1004.240 kb on - strand, within Psest_0926 at 1004.297 kb on - strand, within Psest_0926 at 1004.317 kb on - strand, within Psest_0926 at 1004.317 kb on - strand, within Psest_0926 at 1004.317 kb on - strand, within Psest_0926 at 1004.368 kb on + strand, within Psest_0926 at 1004.369 kb on - strand, within Psest_0926 at 1004.369 kb on - strand, within Psest_0926 at 1004.431 kb on + strand, within Psest_0926 at 1004.431 kb on + strand, within Psest_0926 at 1004.432 kb on - strand, within Psest_0926 at 1004.512 kb on + strand, within Psest_0926 at 1004.545 kb on - strand, within Psest_0926 at 1004.628 kb on + strand, within Psest_0926 at 1004.629 kb on - strand, within Psest_0926 at 1004.629 kb on - strand, within Psest_0926 at 1004.723 kb on + strand, within Psest_0926 at 1004.753 kb on - strand, within Psest_0926 at 1004.753 kb on - strand, within Psest_0926
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 1,002,708 - Psest_0922 0.85 +0.7 1,002,752 - +0.1 1,002,752 - -0.8 1,002,752 - -1.0 1,002,752 - -0.1 1,002,804 + -0.8 1,002,804 + -1.5 1,002,804 + -0.7 1,002,805 - +0.2 1,002,805 - +2.5 1,002,814 + +0.1 1,002,814 + +2.0 1,002,814 + -1.2 1,002,814 + -2.0 1,002,815 - -0.6 1,002,815 - -0.6 1,002,815 - +1.7 1,002,836 + +1.7 1,002,836 + +1.1 1,002,836 + -2.1 1,002,836 + +0.2 1,002,836 + -0.6 1,002,837 - -3.1 1,002,837 - +0.4 1,002,837 - -0.2 1,002,837 - -0.1 1,002,839 - +1.7 1,002,839 - +4.2 1,002,839 - +1.0 1,002,839 - -1.2 1,002,839 - -0.0 1,002,986 + Psest_0923 0.60 -2.0 1,002,986 + Psest_0923 0.60 -0.6 1,002,986 + Psest_0923 0.60 +1.1 1,002,986 + Psest_0923 0.60 -0.4 1,002,986 + Psest_0923 0.60 +0.8 1,002,986 + Psest_0923 0.60 +1.6 1,002,986 + Psest_0923 0.60 +2.5 1,002,986 + Psest_0923 0.60 +1.8 1,002,986 + Psest_0923 0.60 +0.9 1,002,986 + Psest_0923 0.60 -1.2 1,002,986 + Psest_0923 0.60 -2.3 1,002,986 + Psest_0923 0.60 -1.0 1,002,986 + Psest_0923 0.60 +0.6 1,002,987 - Psest_0923 0.61 +1.8 1,002,987 - Psest_0923 0.61 +1.8 1,002,987 - Psest_0923 0.61 -2.7 1,002,987 - Psest_0923 0.61 +1.2 1,002,987 - Psest_0923 0.61 +1.3 1,002,987 - Psest_0923 0.61 +2.1 1,002,987 - Psest_0923 0.61 -1.4 1,002,987 - Psest_0923 0.61 +2.5 1,002,987 - Psest_0923 0.61 +2.7 1,002,987 - Psest_0923 0.61 -2.8 1,002,987 - Psest_0923 0.61 +0.5 1,002,987 - Psest_0923 0.61 -0.8 1,002,987 - Psest_0923 0.61 +1.2 1,002,987 - Psest_0923 0.61 -0.6 1,002,987 - Psest_0923 0.61 -1.3 1,002,987 - Psest_0923 0.61 +0.2 1,002,987 - Psest_0923 0.61 +0.9 1,002,987 - Psest_0923 0.61 +1.6 1,003,051 + Psest_0923 0.84 +2.1 1,003,089 + -1.4 1,003,089 + -0.0 1,003,089 + -2.2 1,003,089 + +0.6 1,003,090 - -1.7 1,003,140 + +2.8 1,003,169 + +0.9 1,003,169 + +2.3 1,003,169 + +1.4 1,003,169 + -1.8 1,003,169 + -2.5 1,003,169 + -0.7 1,003,169 + -2.0 1,003,169 + +0.4 1,003,169 + +0.7 1,003,169 + +2.1 1,003,169 + +0.4 1,003,169 + -3.0 1,003,169 + -1.1 1,003,169 + -2.6 1,003,169 + +0.2 1,003,169 + +0.1 1,003,169 + -0.6 1,003,169 + -1.0 1,003,169 + +0.9 1,003,169 + -0.1 1,003,169 + -1.0 1,003,169 + -0.4 1,003,169 + -0.8 1,003,169 + +0.8 1,003,169 + -1.0 1,003,169 + -1.2 1,003,169 + -2.9 1,003,169 + -0.8 1,003,169 + +2.1 1,003,169 + -3.5 1,003,169 + -1.3 1,003,169 + -0.8 1,003,169 + +0.9 1,003,169 + -0.6 1,003,169 + -2.8 1,003,170 - -2.4 1,003,170 - -1.2 1,003,170 - +0.5 1,003,170 - -1.4 1,003,170 - -1.1 1,003,170 - -0.4 1,003,170 - +1.0 1,003,170 - +2.9 1,003,170 - -0.8 1,003,170 - +0.5 1,003,170 - +1.1 1,003,170 - +0.6 1,003,170 - +3.1 1,003,170 - +0.7 1,003,170 - -0.8 1,003,170 - -1.0 1,003,170 - +1.2 1,003,170 - -0.9 1,003,170 - -1.4 1,003,170 - +3.5 1,003,170 - -1.2 1,003,170 - -1.5 1,003,170 - -0.9 1,003,170 - -2.3 1,003,170 - -0.4 1,003,170 - +0.5 1,003,170 - -2.2 1,003,170 - +0.3 1,003,170 - -0.8 1,003,170 - -0.1 1,003,170 - -0.8 1,003,170 - +1.1 1,003,170 - +3.4 1,003,170 - -1.2 1,003,170 - -2.6 1,003,170 - +1.3 1,003,170 - +1.9 1,003,170 - +0.5 1,003,170 - +1.8 1,003,198 + +1.8 1,003,199 - -1.5 1,003,424 + +1.2 1,003,471 + +1.6 1,003,517 + +0.4 1,003,581 + +1.5 1,003,582 - -1.5 1,003,582 - +2.6 1,003,640 + -3.2 1,003,640 + +2.6 1,003,646 + -1.4 1,003,688 - Psest_0925 0.11 -2.6 1,003,693 - Psest_0925 0.13 +5.1 1,003,693 - Psest_0925 0.13 +1.2 1,003,775 + Psest_0925 0.41 +5.3 1,003,776 - Psest_0925 0.42 -0.9 1,003,776 - Psest_0925 0.42 +0.8 1,003,776 - Psest_0925 0.42 -0.8 1,003,848 + Psest_0925 0.66 -0.2 1,003,849 - Psest_0925 0.67 -0.8 1,004,003 + +2.4 1,004,003 + -2.2 1,004,003 + -1.9 1,004,004 - -1.1 1,004,007 + -3.1 1,004,007 + +2.0 1,004,007 + -2.3 1,004,007 + +1.2 1,004,008 - +3.3 1,004,008 - -0.4 1,004,008 - -1.6 1,004,008 - +2.5 1,004,008 - -1.2 1,004,008 - +2.7 1,004,122 + Psest_0926 0.11 +2.0 1,004,123 - Psest_0926 0.11 +2.7 1,004,150 + Psest_0926 0.14 -0.4 1,004,150 + Psest_0926 0.14 -0.0 1,004,151 - Psest_0926 0.14 -0.1 1,004,220 + Psest_0926 0.22 +2.4 1,004,220 + Psest_0926 0.22 -2.5 1,004,221 - Psest_0926 0.22 -3.4 1,004,221 - Psest_0926 0.22 -1.8 1,004,221 - Psest_0926 0.22 +2.6 1,004,240 - Psest_0926 0.24 +0.6 1,004,297 - Psest_0926 0.30 -1.4 1,004,317 - Psest_0926 0.32 -1.9 1,004,317 - Psest_0926 0.32 +0.4 1,004,317 - Psest_0926 0.32 -1.0 1,004,368 + Psest_0926 0.38 -1.2 1,004,369 - Psest_0926 0.38 -0.4 1,004,369 - Psest_0926 0.38 +1.3 1,004,431 + Psest_0926 0.44 +0.5 1,004,431 + Psest_0926 0.44 -0.6 1,004,432 - Psest_0926 0.45 -2.0 1,004,512 + Psest_0926 0.53 +1.7 1,004,545 - Psest_0926 0.57 +0.5 1,004,628 + Psest_0926 0.66 +1.2 1,004,629 - Psest_0926 0.66 +1.4 1,004,629 - Psest_0926 0.66 +1.0 1,004,723 + Psest_0926 0.76 -1.1 1,004,753 - Psest_0926 0.79 -1.6 1,004,753 - Psest_0926 0.79 +1.5
Or see this region's nucleotide sequence