Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0916 and Psest_0917 are separated by 448 nucleotides Psest_0917 and Psest_0918 are separated by 356 nucleotides
Psest_0916: Psest_0916 - Phage integrase family., at 995,596 to 996,489
_0916
Psest_0917: Psest_0917 - SEC-C motif., at 996,938 to 998,293
_0917
Psest_0918: Psest_0918 - Predicted permease, DMT superfamily, at 998,650 to 999,558
_0918
Position (kb) 996
997
998
999 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 995.958 kb on - strand, within Psest_0916 at 996.021 kb on + strand, within Psest_0916 at 996.021 kb on + strand, within Psest_0916 at 996.021 kb on + strand, within Psest_0916 at 996.021 kb on + strand, within Psest_0916 at 996.022 kb on - strand, within Psest_0916 at 996.022 kb on - strand, within Psest_0916 at 996.022 kb on - strand, within Psest_0916 at 996.022 kb on - strand, within Psest_0916 at 996.022 kb on - strand, within Psest_0916 at 996.022 kb on - strand, within Psest_0916 at 996.022 kb on - strand, within Psest_0916 at 996.023 kb on + strand, within Psest_0916 at 996.023 kb on + strand, within Psest_0916 at 996.023 kb on + strand, within Psest_0916 at 996.024 kb on - strand, within Psest_0916 at 996.024 kb on - strand, within Psest_0916 at 996.024 kb on - strand, within Psest_0916 at 996.126 kb on + strand, within Psest_0916 at 996.126 kb on + strand, within Psest_0916 at 996.146 kb on + strand, within Psest_0916 at 996.147 kb on - strand, within Psest_0916 at 996.214 kb on - strand, within Psest_0916 at 996.214 kb on - strand, within Psest_0916 at 996.214 kb on - strand, within Psest_0916 at 996.214 kb on - strand, within Psest_0916 at 996.217 kb on + strand, within Psest_0916 at 996.218 kb on - strand, within Psest_0916 at 996.218 kb on - strand, within Psest_0916 at 996.218 kb on - strand, within Psest_0916 at 996.283 kb on + strand, within Psest_0916 at 996.283 kb on + strand, within Psest_0916 at 996.283 kb on + strand, within Psest_0916 at 996.283 kb on + strand, within Psest_0916 at 996.283 kb on + strand, within Psest_0916 at 996.283 kb on + strand, within Psest_0916 at 996.284 kb on - strand, within Psest_0916 at 996.402 kb on + strand at 996.402 kb on + strand at 996.402 kb on + strand at 996.402 kb on + strand at 996.402 kb on + strand at 996.402 kb on + strand at 996.402 kb on + strand at 996.402 kb on + strand at 996.403 kb on - strand at 996.403 kb on - strand at 996.403 kb on - strand at 996.442 kb on - strand at 996.465 kb on + strand at 996.465 kb on + strand at 996.465 kb on + strand at 996.465 kb on + strand at 996.465 kb on + strand at 996.544 kb on + strand at 996.544 kb on + strand at 996.545 kb on - strand at 996.549 kb on - strand at 996.579 kb on + strand at 996.579 kb on + strand at 996.580 kb on - strand at 996.605 kb on + strand at 996.609 kb on + strand at 996.609 kb on + strand at 996.609 kb on + strand at 996.609 kb on + strand at 996.609 kb on + strand at 996.610 kb on - strand at 996.610 kb on - strand at 996.612 kb on + strand at 996.613 kb on - strand at 996.751 kb on + strand at 996.788 kb on + strand at 996.789 kb on - strand at 996.789 kb on - strand at 996.789 kb on - strand at 996.851 kb on + strand at 996.851 kb on + strand at 996.854 kb on + strand at 996.860 kb on + strand at 996.861 kb on - strand at 996.885 kb on + strand at 996.885 kb on + strand at 996.885 kb on + strand at 996.886 kb on - strand at 996.966 kb on + strand at 996.966 kb on + strand at 996.967 kb on - strand at 996.967 kb on - strand at 997.084 kb on - strand, within Psest_0917 at 997.302 kb on + strand, within Psest_0917 at 997.302 kb on + strand, within Psest_0917 at 997.303 kb on - strand, within Psest_0917 at 997.314 kb on + strand, within Psest_0917 at 997.314 kb on + strand, within Psest_0917 at 997.315 kb on - strand, within Psest_0917 at 997.486 kb on - strand, within Psest_0917 at 997.530 kb on + strand, within Psest_0917 at 997.530 kb on + strand, within Psest_0917 at 997.530 kb on + strand, within Psest_0917 at 997.531 kb on - strand, within Psest_0917 at 997.543 kb on + strand, within Psest_0917 at 997.543 kb on + strand, within Psest_0917 at 997.543 kb on + strand, within Psest_0917 at 997.544 kb on - strand, within Psest_0917 at 997.558 kb on + strand, within Psest_0917 at 997.565 kb on - strand, within Psest_0917 at 997.567 kb on + strand, within Psest_0917 at 997.593 kb on - strand, within Psest_0917 at 997.634 kb on - strand, within Psest_0917 at 997.682 kb on - strand, within Psest_0917 at 997.704 kb on + strand, within Psest_0917 at 997.768 kb on - strand, within Psest_0917 at 997.878 kb on + strand, within Psest_0917 at 997.884 kb on + strand, within Psest_0917 at 997.884 kb on + strand, within Psest_0917 at 997.884 kb on + strand, within Psest_0917 at 997.884 kb on + strand, within Psest_0917 at 997.885 kb on - strand, within Psest_0917 at 997.888 kb on + strand, within Psest_0917 at 997.952 kb on + strand, within Psest_0917 at 997.990 kb on + strand, within Psest_0917 at 998.018 kb on + strand, within Psest_0917 at 998.018 kb on + strand, within Psest_0917 at 998.022 kb on + strand, within Psest_0917 at 998.023 kb on - strand, within Psest_0917 at 998.055 kb on + strand, within Psest_0917 at 998.080 kb on + strand, within Psest_0917 at 998.081 kb on - strand, within Psest_0917 at 998.081 kb on - strand, within Psest_0917 at 998.089 kb on - strand, within Psest_0917 at 998.089 kb on - strand, within Psest_0917 at 998.089 kb on - strand, within Psest_0917 at 998.129 kb on + strand, within Psest_0917 at 998.129 kb on + strand, within Psest_0917 at 998.130 kb on - strand, within Psest_0917 at 998.270 kb on + strand at 998.270 kb on + strand at 998.271 kb on - strand at 998.271 kb on - strand at 998.271 kb on - strand at 998.325 kb on + strand at 998.354 kb on + strand at 998.354 kb on + strand at 998.354 kb on + strand at 998.354 kb on + strand at 998.447 kb on + strand at 998.486 kb on + strand at 998.486 kb on + strand at 998.486 kb on + strand at 998.486 kb on + strand at 998.487 kb on - strand at 998.668 kb on + strand at 998.668 kb on + strand at 998.668 kb on + strand at 998.668 kb on + strand at 998.668 kb on + strand at 998.668 kb on + strand at 998.668 kb on + strand at 998.668 kb on + strand at 998.668 kb on + strand at 998.668 kb on + strand at 998.669 kb on - strand at 998.669 kb on - strand at 998.669 kb on - strand at 998.669 kb on - strand at 998.669 kb on - strand at 998.669 kb on - strand at 998.669 kb on - strand at 998.669 kb on - strand at 998.669 kb on - strand at 998.669 kb on - strand at 998.669 kb on - strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.673 kb on - strand at 998.756 kb on + strand, within Psest_0918 at 998.756 kb on + strand, within Psest_0918 at 998.756 kb on + strand, within Psest_0918 at 998.756 kb on + strand, within Psest_0918 at 998.767 kb on + strand, within Psest_0918 at 998.861 kb on + strand, within Psest_0918 at 998.861 kb on + strand, within Psest_0918 at 998.862 kb on - strand, within Psest_0918 at 998.862 kb on - strand, within Psest_0918 at 998.862 kb on - strand, within Psest_0918 at 998.867 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 999.047 kb on - strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.128 kb on - strand, within Psest_0918 at 999.128 kb on - strand, within Psest_0918 at 999.128 kb on - strand, within Psest_0918 at 999.156 kb on + strand, within Psest_0918 at 999.156 kb on + strand, within Psest_0918 at 999.156 kb on + strand, within Psest_0918 at 999.157 kb on - strand, within Psest_0918 at 999.160 kb on + strand, within Psest_0918 at 999.160 kb on + strand, within Psest_0918 at 999.160 kb on + strand, within Psest_0918 at 999.160 kb on + strand, within Psest_0918 at 999.161 kb on - strand, within Psest_0918 at 999.196 kb on - strand, within Psest_0918 at 999.237 kb on + strand, within Psest_0918
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 995,958 - Psest_0916 0.40 +0.5 996,021 + Psest_0916 0.48 -0.5 996,021 + Psest_0916 0.48 -0.8 996,021 + Psest_0916 0.48 +1.5 996,021 + Psest_0916 0.48 +0.5 996,022 - Psest_0916 0.48 +0.5 996,022 - Psest_0916 0.48 -3.2 996,022 - Psest_0916 0.48 +0.4 996,022 - Psest_0916 0.48 -1.2 996,022 - Psest_0916 0.48 -1.7 996,022 - Psest_0916 0.48 +3.1 996,022 - Psest_0916 0.48 +2.8 996,023 + Psest_0916 0.48 +1.7 996,023 + Psest_0916 0.48 +2.6 996,023 + Psest_0916 0.48 -1.3 996,024 - Psest_0916 0.48 -0.7 996,024 - Psest_0916 0.48 -0.3 996,024 - Psest_0916 0.48 -1.9 996,126 + Psest_0916 0.59 -2.6 996,126 + Psest_0916 0.59 -0.0 996,146 + Psest_0916 0.62 +1.7 996,147 - Psest_0916 0.62 -0.6 996,214 - Psest_0916 0.69 +0.4 996,214 - Psest_0916 0.69 +0.2 996,214 - Psest_0916 0.69 +2.0 996,214 - Psest_0916 0.69 -2.0 996,217 + Psest_0916 0.69 +2.3 996,218 - Psest_0916 0.70 +1.4 996,218 - Psest_0916 0.70 -0.0 996,218 - Psest_0916 0.70 +1.5 996,283 + Psest_0916 0.77 -0.2 996,283 + Psest_0916 0.77 +0.6 996,283 + Psest_0916 0.77 +0.6 996,283 + Psest_0916 0.77 +0.7 996,283 + Psest_0916 0.77 +1.3 996,283 + Psest_0916 0.77 +0.8 996,284 - Psest_0916 0.77 -0.6 996,402 + -2.5 996,402 + -0.3 996,402 + +0.5 996,402 + +3.9 996,402 + -0.3 996,402 + +1.4 996,402 + +0.3 996,402 + -2.6 996,403 - +2.0 996,403 - -0.1 996,403 - +1.7 996,442 - -1.0 996,465 + +2.9 996,465 + +0.5 996,465 + -1.9 996,465 + +1.0 996,465 + -0.5 996,544 + +1.0 996,544 + +2.4 996,545 - +2.0 996,549 - +2.1 996,579 + -2.3 996,579 + -1.0 996,580 - -2.6 996,605 + +1.1 996,609 + -0.2 996,609 + +1.4 996,609 + -0.3 996,609 + +4.4 996,609 + +3.4 996,610 - +0.3 996,610 - -2.7 996,612 + +2.1 996,613 - +3.3 996,751 + +1.2 996,788 + -2.3 996,789 - -0.6 996,789 - -1.9 996,789 - +1.1 996,851 + -2.1 996,851 + +0.4 996,854 + -3.6 996,860 + -1.0 996,861 - -0.8 996,885 + -0.3 996,885 + +0.5 996,885 + +0.2 996,886 - -2.0 996,966 + -1.3 996,966 + -0.8 996,967 - -1.6 996,967 - +0.3 997,084 - Psest_0917 0.11 -0.2 997,302 + Psest_0917 0.27 -1.0 997,302 + Psest_0917 0.27 +1.3 997,303 - Psest_0917 0.27 +1.5 997,314 + Psest_0917 0.28 +0.4 997,314 + Psest_0917 0.28 +4.7 997,315 - Psest_0917 0.28 +1.2 997,486 - Psest_0917 0.40 +1.0 997,530 + Psest_0917 0.44 -2.3 997,530 + Psest_0917 0.44 -1.6 997,530 + Psest_0917 0.44 +2.8 997,531 - Psest_0917 0.44 -3.3 997,543 + Psest_0917 0.45 +2.2 997,543 + Psest_0917 0.45 +0.3 997,543 + Psest_0917 0.45 -2.5 997,544 - Psest_0917 0.45 +1.1 997,558 + Psest_0917 0.46 -2.0 997,565 - Psest_0917 0.46 +1.6 997,567 + Psest_0917 0.46 +0.5 997,593 - Psest_0917 0.48 +1.2 997,634 - Psest_0917 0.51 -0.5 997,682 - Psest_0917 0.55 +0.2 997,704 + Psest_0917 0.56 -2.3 997,768 - Psest_0917 0.61 +0.4 997,878 + Psest_0917 0.69 +0.7 997,884 + Psest_0917 0.70 +3.2 997,884 + Psest_0917 0.70 -1.0 997,884 + Psest_0917 0.70 -2.0 997,884 + Psest_0917 0.70 -1.2 997,885 - Psest_0917 0.70 -2.5 997,888 + Psest_0917 0.70 -2.3 997,952 + Psest_0917 0.75 -1.1 997,990 + Psest_0917 0.78 +0.1 998,018 + Psest_0917 0.80 -1.9 998,018 + Psest_0917 0.80 -0.5 998,022 + Psest_0917 0.80 +0.4 998,023 - Psest_0917 0.80 -1.3 998,055 + Psest_0917 0.82 +3.4 998,080 + Psest_0917 0.84 +1.1 998,081 - Psest_0917 0.84 -0.8 998,081 - Psest_0917 0.84 +1.2 998,089 - Psest_0917 0.85 +2.5 998,089 - Psest_0917 0.85 +1.9 998,089 - Psest_0917 0.85 +1.4 998,129 + Psest_0917 0.88 +1.9 998,129 + Psest_0917 0.88 -1.0 998,130 - Psest_0917 0.88 -0.9 998,270 + -1.2 998,270 + -0.8 998,271 - -1.9 998,271 - -0.5 998,271 - +2.5 998,325 + -3.1 998,354 + -0.8 998,354 + +2.0 998,354 + +0.1 998,354 + +0.6 998,447 + +0.5 998,486 + -2.3 998,486 + +3.0 998,486 + +0.6 998,486 + +1.5 998,487 - -3.1 998,668 + -1.2 998,668 + +1.9 998,668 + -1.4 998,668 + -0.6 998,668 + -1.3 998,668 + -1.8 998,668 + -1.3 998,668 + +0.5 998,668 + -1.0 998,668 + +0.1 998,669 - -0.6 998,669 - +1.0 998,669 - -0.9 998,669 - -2.9 998,669 - +0.2 998,669 - -0.3 998,669 - -0.9 998,669 - +1.3 998,669 - -0.3 998,669 - +0.6 998,669 - +2.3 998,672 + +2.4 998,672 + -1.5 998,672 + -1.2 998,672 + -1.6 998,672 + +1.9 998,672 + -1.7 998,672 + +4.1 998,672 + +0.5 998,672 + -1.2 998,672 + +1.5 998,672 + +3.0 998,673 - +1.2 998,756 + Psest_0918 0.12 -1.5 998,756 + Psest_0918 0.12 +1.5 998,756 + Psest_0918 0.12 +3.0 998,756 + Psest_0918 0.12 +0.7 998,767 + Psest_0918 0.13 +0.6 998,861 + Psest_0918 0.23 -0.1 998,861 + Psest_0918 0.23 +3.7 998,862 - Psest_0918 0.23 -1.8 998,862 - Psest_0918 0.23 +1.1 998,862 - Psest_0918 0.23 -1.2 998,867 + Psest_0918 0.24 +2.3 998,954 + Psest_0918 0.33 -0.6 998,954 + Psest_0918 0.33 -0.3 998,954 + Psest_0918 0.33 -2.8 998,954 + Psest_0918 0.33 -1.3 998,954 + Psest_0918 0.33 +1.5 998,954 + Psest_0918 0.33 +0.3 998,954 + Psest_0918 0.33 +1.6 998,954 + Psest_0918 0.33 +1.0 998,954 + Psest_0918 0.33 +0.5 998,954 + Psest_0918 0.33 -1.0 998,954 + Psest_0918 0.33 -0.4 998,954 + Psest_0918 0.33 +1.5 998,954 + Psest_0918 0.33 +1.8 998,955 - Psest_0918 0.34 -0.7 998,955 - Psest_0918 0.34 -0.6 998,955 - Psest_0918 0.34 -2.0 998,955 - Psest_0918 0.34 +1.0 998,955 - Psest_0918 0.34 -1.6 998,955 - Psest_0918 0.34 +3.1 998,955 - Psest_0918 0.34 +0.5 998,955 - Psest_0918 0.34 +3.3 998,955 - Psest_0918 0.34 -0.9 998,955 - Psest_0918 0.34 -1.5 998,955 - Psest_0918 0.34 +0.2 998,955 - Psest_0918 0.34 +2.1 998,955 - Psest_0918 0.34 -0.2 999,047 - Psest_0918 0.44 -2.5 999,127 + Psest_0918 0.52 -3.1 999,127 + Psest_0918 0.52 -0.0 999,127 + Psest_0918 0.52 -2.8 999,127 + Psest_0918 0.52 -2.8 999,127 + Psest_0918 0.52 +0.7 999,127 + Psest_0918 0.52 +0.8 999,127 + Psest_0918 0.52 -1.0 999,127 + Psest_0918 0.52 -0.0 999,127 + Psest_0918 0.52 -1.2 999,127 + Psest_0918 0.52 +0.0 999,127 + Psest_0918 0.52 -4.3 999,127 + Psest_0918 0.52 +3.6 999,128 - Psest_0918 0.53 +2.1 999,128 - Psest_0918 0.53 -0.3 999,128 - Psest_0918 0.53 -0.5 999,156 + Psest_0918 0.56 +0.6 999,156 + Psest_0918 0.56 -0.4 999,156 + Psest_0918 0.56 +1.3 999,157 - Psest_0918 0.56 -0.1 999,160 + Psest_0918 0.56 -0.1 999,160 + Psest_0918 0.56 +1.0 999,160 + Psest_0918 0.56 +1.4 999,160 + Psest_0918 0.56 -0.5 999,161 - Psest_0918 0.56 -1.5 999,196 - Psest_0918 0.60 -0.9 999,237 + Psest_0918 0.65 +1.6
Or see this region's nucleotide sequence