Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0804 and Psest_0805 are separated by 23 nucleotides Psest_0805 and Psest_0806 overlap by 4 nucleotides Psest_0806 and Psest_0807 overlap by 4 nucleotides Psest_0807 and Psest_0808 overlap by 4 nucleotides
Psest_0804: Psest_0804 - hypothetical protein, at 878,428 to 881,445
_0804
Psest_0805: Psest_0805 - hypothetical protein, at 881,469 to 881,942
_0805
Psest_0806: Psest_0806 - prepilin-type N-terminal cleavage/methylation domain, at 881,939 to 883,054
_0806
Psest_0807: Psest_0807 - type IV pilus modification protein PilV, at 883,051 to 883,620
_0807
Psest_0808: Psest_0808 - prepilin-type N-terminal cleavage/methylation domain, at 883,617 to 884,009
_0808
Position (kb) 881
882
883
884 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 881.027 kb on + strand, within Psest_0804 at 881.027 kb on + strand, within Psest_0804 at 881.027 kb on + strand, within Psest_0804 at 881.027 kb on + strand, within Psest_0804 at 881.033 kb on + strand, within Psest_0804 at 881.033 kb on + strand, within Psest_0804 at 881.033 kb on + strand, within Psest_0804 at 881.033 kb on + strand, within Psest_0804 at 881.162 kb on + strand at 881.162 kb on + strand at 881.162 kb on + strand at 881.163 kb on - strand at 881.163 kb on - strand at 881.203 kb on - strand at 881.304 kb on + strand at 881.305 kb on - strand at 881.305 kb on - strand at 881.451 kb on + strand at 881.451 kb on + strand at 881.624 kb on + strand, within Psest_0805 at 881.682 kb on + strand, within Psest_0805 at 881.689 kb on + strand, within Psest_0805 at 881.689 kb on + strand, within Psest_0805 at 881.689 kb on + strand, within Psest_0805 at 881.689 kb on + strand, within Psest_0805 at 881.991 kb on + strand at 881.992 kb on - strand at 881.992 kb on - strand at 882.048 kb on + strand at 882.048 kb on + strand at 882.048 kb on + strand at 882.048 kb on + strand at 882.048 kb on + strand at 882.049 kb on - strand at 882.049 kb on - strand at 882.049 kb on - strand at 882.049 kb on - strand at 882.244 kb on - strand, within Psest_0806 at 882.308 kb on - strand, within Psest_0806 at 882.308 kb on - strand, within Psest_0806 at 882.423 kb on + strand, within Psest_0806 at 882.423 kb on + strand, within Psest_0806 at 882.423 kb on + strand, within Psest_0806 at 882.423 kb on + strand, within Psest_0806 at 882.423 kb on + strand, within Psest_0806 at 882.423 kb on + strand, within Psest_0806 at 882.423 kb on + strand, within Psest_0806 at 882.424 kb on - strand, within Psest_0806 at 882.424 kb on - strand, within Psest_0806 at 882.424 kb on - strand, within Psest_0806 at 882.424 kb on - strand, within Psest_0806 at 882.424 kb on - strand, within Psest_0806 at 882.426 kb on + strand, within Psest_0806 at 882.426 kb on + strand, within Psest_0806 at 882.510 kb on + strand, within Psest_0806 at 882.546 kb on + strand, within Psest_0806 at 882.546 kb on + strand, within Psest_0806 at 882.547 kb on - strand, within Psest_0806 at 882.611 kb on + strand, within Psest_0806 at 882.650 kb on + strand, within Psest_0806 at 882.672 kb on - strand, within Psest_0806 at 882.681 kb on + strand, within Psest_0806 at 882.717 kb on + strand, within Psest_0806 at 882.718 kb on - strand, within Psest_0806 at 882.736 kb on + strand, within Psest_0806 at 882.737 kb on - strand, within Psest_0806 at 882.737 kb on - strand, within Psest_0806 at 882.738 kb on + strand, within Psest_0806 at 882.739 kb on - strand, within Psest_0806 at 882.758 kb on + strand, within Psest_0806 at 882.792 kb on + strand, within Psest_0806 at 882.792 kb on + strand, within Psest_0806 at 882.940 kb on - strand, within Psest_0806 at 883.081 kb on + strand at 883.153 kb on + strand, within Psest_0807 at 883.154 kb on - strand, within Psest_0807 at 883.154 kb on - strand, within Psest_0807 at 883.211 kb on + strand, within Psest_0807 at 883.211 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.400 kb on + strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.401 kb on - strand, within Psest_0807 at 883.478 kb on + strand, within Psest_0807 at 883.478 kb on + strand, within Psest_0807 at 883.479 kb on - strand, within Psest_0807 at 883.479 kb on - strand, within Psest_0807 at 883.479 kb on - strand, within Psest_0807 at 883.479 kb on - strand, within Psest_0807 at 883.479 kb on - strand, within Psest_0807 at 883.711 kb on + strand, within Psest_0808 at 883.711 kb on + strand, within Psest_0808 at 883.740 kb on - strand, within Psest_0808 at 883.753 kb on + strand, within Psest_0808 at 883.754 kb on - strand, within Psest_0808 at 883.754 kb on - strand, within Psest_0808 at 883.796 kb on + strand, within Psest_0808 at 883.796 kb on + strand, within Psest_0808 at 883.797 kb on - strand, within Psest_0808 at 883.816 kb on + strand, within Psest_0808 at 883.816 kb on + strand, within Psest_0808 at 883.906 kb on + strand, within Psest_0808 at 883.906 kb on + strand, within Psest_0808 at 883.907 kb on - strand, within Psest_0808 at 883.907 kb on - strand, within Psest_0808 at 883.968 kb on + strand, within Psest_0808 at 883.968 kb on + strand, within Psest_0808 at 883.969 kb on - strand, within Psest_0808 at 883.969 kb on - strand, within Psest_0808 at 883.969 kb on - strand, within Psest_0808 at 883.969 kb on - strand, within Psest_0808 at 883.997 kb on + strand at 883.997 kb on + strand at 883.997 kb on + strand at 883.997 kb on + strand at 883.997 kb on + strand at 883.997 kb on + strand at 883.997 kb on + strand at 883.998 kb on - strand at 883.998 kb on - strand at 883.998 kb on - strand at 883.998 kb on - strand at 883.998 kb on - strand at 883.998 kb on - strand at 883.998 kb on - strand at 884.034 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 881,027 + Psest_0804 0.86 -0.9 881,027 + Psest_0804 0.86 -0.4 881,027 + Psest_0804 0.86 +2.4 881,027 + Psest_0804 0.86 +0.1 881,033 + Psest_0804 0.86 -2.1 881,033 + Psest_0804 0.86 +2.7 881,033 + Psest_0804 0.86 -3.6 881,033 + Psest_0804 0.86 +1.9 881,162 + -1.6 881,162 + +0.1 881,162 + -0.4 881,163 - -0.3 881,163 - -0.4 881,203 - -3.0 881,304 + -4.0 881,305 - +1.2 881,305 - +0.2 881,451 + +0.7 881,451 + -3.7 881,624 + Psest_0805 0.33 -3.7 881,682 + Psest_0805 0.45 -1.8 881,689 + Psest_0805 0.46 -0.1 881,689 + Psest_0805 0.46 -2.5 881,689 + Psest_0805 0.46 +1.0 881,689 + Psest_0805 0.46 +0.7 881,991 + +0.2 881,992 - +2.1 881,992 - +0.6 882,048 + -1.4 882,048 + -0.3 882,048 + -2.6 882,048 + -1.2 882,048 + -1.9 882,049 - -2.6 882,049 - +0.4 882,049 - -0.5 882,049 - +0.3 882,244 - Psest_0806 0.27 -0.4 882,308 - Psest_0806 0.33 -2.3 882,308 - Psest_0806 0.33 -1.9 882,423 + Psest_0806 0.43 +0.5 882,423 + Psest_0806 0.43 +2.8 882,423 + Psest_0806 0.43 -2.4 882,423 + Psest_0806 0.43 +1.0 882,423 + Psest_0806 0.43 -0.5 882,423 + Psest_0806 0.43 -1.5 882,423 + Psest_0806 0.43 -1.6 882,424 - Psest_0806 0.43 -1.1 882,424 - Psest_0806 0.43 -1.6 882,424 - Psest_0806 0.43 -1.9 882,424 - Psest_0806 0.43 -2.0 882,424 - Psest_0806 0.43 +0.2 882,426 + Psest_0806 0.44 +1.4 882,426 + Psest_0806 0.44 -0.5 882,510 + Psest_0806 0.51 +1.9 882,546 + Psest_0806 0.54 -1.7 882,546 + Psest_0806 0.54 -1.8 882,547 - Psest_0806 0.54 -0.4 882,611 + Psest_0806 0.60 -0.2 882,650 + Psest_0806 0.64 -0.1 882,672 - Psest_0806 0.66 +1.2 882,681 + Psest_0806 0.66 -1.4 882,717 + Psest_0806 0.70 +0.3 882,718 - Psest_0806 0.70 -0.2 882,736 + Psest_0806 0.71 -0.9 882,737 - Psest_0806 0.72 -0.7 882,737 - Psest_0806 0.72 -0.1 882,738 + Psest_0806 0.72 -2.1 882,739 - Psest_0806 0.72 +2.2 882,758 + Psest_0806 0.73 -2.2 882,792 + Psest_0806 0.76 -1.8 882,792 + Psest_0806 0.76 +0.4 882,940 - Psest_0806 0.90 +1.0 883,081 + -3.7 883,153 + Psest_0807 0.18 -1.6 883,154 - Psest_0807 0.18 -1.0 883,154 - Psest_0807 0.18 -1.6 883,211 + Psest_0807 0.28 +2.4 883,211 + Psest_0807 0.28 +0.1 883,400 + Psest_0807 0.61 -1.1 883,400 + Psest_0807 0.61 -1.9 883,400 + Psest_0807 0.61 +1.2 883,400 + Psest_0807 0.61 +1.2 883,400 + Psest_0807 0.61 -0.2 883,400 + Psest_0807 0.61 -1.2 883,400 + Psest_0807 0.61 +1.4 883,400 + Psest_0807 0.61 -2.0 883,400 + Psest_0807 0.61 +2.8 883,400 + Psest_0807 0.61 -1.6 883,400 + Psest_0807 0.61 +0.1 883,401 - Psest_0807 0.61 -0.7 883,401 - Psest_0807 0.61 -4.4 883,401 - Psest_0807 0.61 +1.9 883,401 - Psest_0807 0.61 -0.4 883,401 - Psest_0807 0.61 +0.4 883,401 - Psest_0807 0.61 -1.4 883,401 - Psest_0807 0.61 -0.0 883,401 - Psest_0807 0.61 +1.4 883,401 - Psest_0807 0.61 +2.0 883,401 - Psest_0807 0.61 -0.2 883,401 - Psest_0807 0.61 +1.0 883,478 + Psest_0807 0.75 -0.6 883,478 + Psest_0807 0.75 -3.3 883,479 - Psest_0807 0.75 -0.3 883,479 - Psest_0807 0.75 +2.2 883,479 - Psest_0807 0.75 -2.2 883,479 - Psest_0807 0.75 +2.6 883,479 - Psest_0807 0.75 +1.8 883,711 + Psest_0808 0.24 -3.2 883,711 + Psest_0808 0.24 +0.3 883,740 - Psest_0808 0.31 -2.4 883,753 + Psest_0808 0.35 +2.6 883,754 - Psest_0808 0.35 +0.0 883,754 - Psest_0808 0.35 -1.7 883,796 + Psest_0808 0.46 +0.0 883,796 + Psest_0808 0.46 -1.6 883,797 - Psest_0808 0.46 +0.3 883,816 + Psest_0808 0.51 -2.6 883,816 + Psest_0808 0.51 -1.2 883,906 + Psest_0808 0.74 +0.7 883,906 + Psest_0808 0.74 -1.2 883,907 - Psest_0808 0.74 +1.5 883,907 - Psest_0808 0.74 +0.1 883,968 + Psest_0808 0.89 +1.4 883,968 + Psest_0808 0.89 -0.6 883,969 - Psest_0808 0.90 +1.8 883,969 - Psest_0808 0.90 +0.7 883,969 - Psest_0808 0.90 -0.4 883,969 - Psest_0808 0.90 +1.0 883,997 + -2.8 883,997 + +1.3 883,997 + -1.0 883,997 + -2.3 883,997 + -2.2 883,997 + +0.2 883,997 + -1.5 883,998 - -1.4 883,998 - -1.8 883,998 - -0.7 883,998 - -1.7 883,998 - -0.9 883,998 - -0.3 883,998 - -2.3 884,034 + +0.7
Or see this region's nucleotide sequence