Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0745 and Psest_0746 are separated by 49 nucleotides Psest_0746 and Psest_0747 overlap by 1 nucleotides Psest_0747 and Psest_0748 are separated by 9 nucleotides Psest_0748 and Psest_0749 are separated by 359 nucleotides
Psest_0745: Psest_0745 - Domain of Unknown Function (DUF326)., at 817,937 to 818,266
_0745
Psest_0746: Psest_0746 - Protein of unknown function (DUF1826)., at 818,316 to 818,960
_0746
Psest_0747: Psest_0747 - Putative GTPases (G3E family), at 818,960 to 820,162
_0747
Psest_0748: Psest_0748 - RNA polymerase-binding protein DksA, at 820,172 to 820,576
_0748
Psest_0749: Psest_0749 - Transcriptional regulator, at 820,936 to 821,646
_0749
Position (kb) 818
819
820
821 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 818.551 kb on + strand, within Psest_0746 at 818.551 kb on + strand, within Psest_0746 at 818.552 kb on - strand, within Psest_0746 at 818.552 kb on - strand, within Psest_0746 at 818.552 kb on - strand, within Psest_0746 at 818.568 kb on + strand, within Psest_0746 at 818.583 kb on + strand, within Psest_0746 at 818.583 kb on + strand, within Psest_0746 at 818.583 kb on + strand, within Psest_0746 at 818.583 kb on + strand, within Psest_0746 at 818.583 kb on + strand, within Psest_0746 at 818.583 kb on + strand, within Psest_0746 at 818.584 kb on - strand, within Psest_0746 at 818.584 kb on - strand, within Psest_0746 at 818.584 kb on - strand, within Psest_0746 at 818.584 kb on - strand, within Psest_0746 at 818.584 kb on - strand, within Psest_0746 at 818.584 kb on - strand, within Psest_0746 at 818.584 kb on - strand, within Psest_0746 at 818.898 kb on + strand at 818.898 kb on + strand at 818.898 kb on + strand at 818.899 kb on - strand at 818.899 kb on - strand at 818.899 kb on - strand at 819.222 kb on + strand, within Psest_0747 at 819.223 kb on - strand, within Psest_0747 at 819.223 kb on - strand, within Psest_0747 at 819.348 kb on + strand, within Psest_0747 at 819.348 kb on + strand, within Psest_0747 at 819.348 kb on + strand, within Psest_0747 at 819.349 kb on - strand, within Psest_0747 at 819.349 kb on - strand, within Psest_0747 at 819.349 kb on - strand, within Psest_0747 at 819.349 kb on - strand, within Psest_0747 at 819.384 kb on + strand, within Psest_0747 at 819.405 kb on + strand, within Psest_0747 at 819.405 kb on + strand, within Psest_0747 at 819.405 kb on + strand, within Psest_0747 at 819.405 kb on + strand, within Psest_0747 at 820.536 kb on + strand at 820.536 kb on + strand at 820.536 kb on + strand at 820.537 kb on - strand at 820.537 kb on - strand at 820.537 kb on - strand at 820.537 kb on - strand at 820.558 kb on + strand at 820.629 kb on + strand at 820.629 kb on + strand at 820.629 kb on + strand at 820.630 kb on - strand at 820.630 kb on - strand at 820.630 kb on - strand at 820.630 kb on - strand at 820.780 kb on + strand at 820.900 kb on - strand at 820.905 kb on - strand at 821.049 kb on + strand, within Psest_0749 at 821.050 kb on - strand, within Psest_0749
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 818,551 + Psest_0746 0.36 -1.0 818,551 + Psest_0746 0.36 +1.1 818,552 - Psest_0746 0.37 +0.7 818,552 - Psest_0746 0.37 +1.4 818,552 - Psest_0746 0.37 -1.6 818,568 + Psest_0746 0.39 -1.0 818,583 + Psest_0746 0.41 -0.6 818,583 + Psest_0746 0.41 +0.6 818,583 + Psest_0746 0.41 +2.4 818,583 + Psest_0746 0.41 -1.0 818,583 + Psest_0746 0.41 +3.5 818,583 + Psest_0746 0.41 -1.1 818,584 - Psest_0746 0.42 -0.6 818,584 - Psest_0746 0.42 +0.5 818,584 - Psest_0746 0.42 -3.3 818,584 - Psest_0746 0.42 +1.1 818,584 - Psest_0746 0.42 -0.9 818,584 - Psest_0746 0.42 -0.6 818,584 - Psest_0746 0.42 -2.3 818,898 + -1.2 818,898 + +1.8 818,898 + +2.3 818,899 - +0.2 818,899 - -2.7 818,899 - -0.9 819,222 + Psest_0747 0.22 -1.6 819,223 - Psest_0747 0.22 -2.0 819,223 - Psest_0747 0.22 -0.0 819,348 + Psest_0747 0.32 -3.2 819,348 + Psest_0747 0.32 -1.8 819,348 + Psest_0747 0.32 -2.3 819,349 - Psest_0747 0.32 -1.3 819,349 - Psest_0747 0.32 -1.7 819,349 - Psest_0747 0.32 +1.5 819,349 - Psest_0747 0.32 -2.1 819,384 + Psest_0747 0.35 +2.1 819,405 + Psest_0747 0.37 -1.1 819,405 + Psest_0747 0.37 -2.6 819,405 + Psest_0747 0.37 -2.9 819,405 + Psest_0747 0.37 -0.8 820,536 + -2.7 820,536 + -3.6 820,536 + -0.8 820,537 - +0.2 820,537 - -1.2 820,537 - -0.2 820,537 - -0.5 820,558 + -1.6 820,629 + -0.9 820,629 + -2.1 820,629 + +0.3 820,630 - -0.9 820,630 - -2.6 820,630 - -2.7 820,630 - -3.9 820,780 + -1.7 820,900 - +0.3 820,905 - +1.5 821,049 + Psest_0749 0.16 -1.1 821,050 - Psest_0749 0.16 -1.0
Or see this region's nucleotide sequence