Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0477 and Psest_0478 are separated by 57 nucleotides Psest_0478 and Psest_0479 are separated by 217 nucleotides Psest_0479 and Psest_0480 overlap by 4 nucleotides
Psest_0477: Psest_0477 - thiamine biosynthesis protein ThiC, at 526,744 to 528,624
_0477
Psest_0478: Psest_0478 - Protein of unknown function (DUF3298)., at 528,682 to 529,419
_0478
Psest_0479: Psest_0479 - nudix-type nucleoside diphosphatase, YffH/AdpP family, at 529,637 to 530,254
_0479
Psest_0480: Psest_0480 - Uncharacterized protein conserved in bacteria, at 530,251 to 530,697
_0480
Position (kb)
528
529
530 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 527.701 kb on + strand, within Psest_0477 at 527.701 kb on + strand, within Psest_0477 at 527.701 kb on + strand, within Psest_0477 at 527.701 kb on + strand, within Psest_0477 at 527.702 kb on - strand, within Psest_0477 at 527.702 kb on - strand, within Psest_0477 at 527.702 kb on - strand, within Psest_0477 at 527.702 kb on - strand, within Psest_0477 at 527.702 kb on - strand, within Psest_0477 at 527.702 kb on - strand, within Psest_0477 at 527.724 kb on - strand, within Psest_0477 at 527.735 kb on - strand, within Psest_0477 at 527.872 kb on + strand, within Psest_0477 at 528.094 kb on + strand, within Psest_0477 at 528.094 kb on + strand, within Psest_0477 at 528.094 kb on + strand, within Psest_0477 at 528.094 kb on + strand, within Psest_0477 at 528.094 kb on + strand, within Psest_0477 at 528.094 kb on + strand, within Psest_0477 at 528.095 kb on - strand, within Psest_0477 at 528.199 kb on + strand, within Psest_0477 at 528.200 kb on - strand, within Psest_0477 at 528.265 kb on + strand, within Psest_0477 at 528.265 kb on + strand, within Psest_0477 at 528.265 kb on + strand, within Psest_0477 at 528.265 kb on + strand, within Psest_0477 at 528.265 kb on + strand, within Psest_0477 at 528.265 kb on + strand, within Psest_0477 at 528.265 kb on + strand, within Psest_0477 at 528.266 kb on - strand, within Psest_0477 at 528.514 kb on + strand at 528.515 kb on - strand at 528.610 kb on + strand at 528.610 kb on + strand at 528.610 kb on + strand at 528.610 kb on + strand at 528.610 kb on + strand at 528.611 kb on - strand at 528.611 kb on - strand at 528.611 kb on - strand at 528.611 kb on - strand at 528.611 kb on - strand at 528.611 kb on - strand at 528.611 kb on - strand at 528.611 kb on - strand at 528.623 kb on - strand at 528.690 kb on - strand at 528.690 kb on - strand at 528.770 kb on + strand, within Psest_0478 at 528.770 kb on + strand, within Psest_0478 at 528.770 kb on + strand, within Psest_0478 at 528.770 kb on + strand, within Psest_0478 at 528.771 kb on - strand, within Psest_0478 at 528.771 kb on - strand, within Psest_0478 at 528.771 kb on - strand, within Psest_0478 at 528.771 kb on - strand, within Psest_0478 at 528.771 kb on - strand, within Psest_0478 at 528.779 kb on + strand, within Psest_0478 at 528.779 kb on + strand, within Psest_0478 at 528.779 kb on + strand, within Psest_0478 at 528.779 kb on + strand, within Psest_0478 at 528.780 kb on - strand, within Psest_0478 at 528.780 kb on - strand, within Psest_0478 at 528.780 kb on - strand, within Psest_0478 at 528.780 kb on - strand, within Psest_0478 at 528.983 kb on + strand, within Psest_0478 at 528.983 kb on + strand, within Psest_0478 at 528.983 kb on + strand, within Psest_0478 at 528.984 kb on - strand, within Psest_0478 at 528.984 kb on - strand, within Psest_0478 at 529.028 kb on + strand, within Psest_0478 at 529.028 kb on + strand, within Psest_0478 at 529.028 kb on + strand, within Psest_0478 at 529.028 kb on + strand, within Psest_0478 at 529.028 kb on + strand, within Psest_0478 at 529.028 kb on + strand, within Psest_0478 at 529.028 kb on + strand, within Psest_0478 at 529.029 kb on - strand, within Psest_0478 at 529.029 kb on - strand, within Psest_0478 at 529.029 kb on - strand, within Psest_0478 at 529.029 kb on - strand, within Psest_0478 at 529.029 kb on - strand, within Psest_0478 at 529.034 kb on + strand, within Psest_0478 at 529.034 kb on + strand, within Psest_0478 at 529.034 kb on + strand, within Psest_0478 at 529.034 kb on + strand, within Psest_0478 at 529.034 kb on + strand, within Psest_0478 at 529.035 kb on - strand, within Psest_0478 at 529.035 kb on - strand, within Psest_0478 at 529.035 kb on - strand, within Psest_0478 at 529.035 kb on - strand, within Psest_0478 at 529.094 kb on + strand, within Psest_0478 at 529.094 kb on + strand, within Psest_0478 at 529.095 kb on - strand, within Psest_0478 at 529.095 kb on - strand, within Psest_0478 at 529.095 kb on - strand, within Psest_0478 at 529.139 kb on + strand, within Psest_0478 at 529.140 kb on - strand, within Psest_0478 at 529.140 kb on - strand, within Psest_0478 at 529.140 kb on - strand, within Psest_0478 at 529.403 kb on + strand at 529.403 kb on + strand at 529.403 kb on + strand at 529.403 kb on + strand at 529.403 kb on + strand at 529.403 kb on + strand at 529.403 kb on + strand at 529.403 kb on + strand at 529.403 kb on + strand at 529.404 kb on - strand at 529.404 kb on - strand at 529.404 kb on - strand at 529.404 kb on - strand at 529.404 kb on - strand at 529.404 kb on - strand at 529.404 kb on - strand at 529.782 kb on + strand, within Psest_0479 at 529.803 kb on + strand, within Psest_0479 at 529.804 kb on - strand, within Psest_0479 at 529.804 kb on - strand, within Psest_0479 at 529.804 kb on - strand, within Psest_0479 at 530.012 kb on + strand, within Psest_0479 at 530.012 kb on + strand, within Psest_0479 at 530.048 kb on + strand, within Psest_0479 at 530.048 kb on + strand, within Psest_0479 at 530.048 kb on + strand, within Psest_0479 at 530.048 kb on + strand, within Psest_0479 at 530.048 kb on + strand, within Psest_0479 at 530.048 kb on + strand, within Psest_0479 at 530.049 kb on - strand, within Psest_0479 at 530.049 kb on - strand, within Psest_0479 at 530.049 kb on - strand, within Psest_0479 at 530.049 kb on - strand, within Psest_0479 at 530.049 kb on - strand, within Psest_0479 at 530.049 kb on - strand, within Psest_0479 at 530.054 kb on + strand, within Psest_0479 at 530.054 kb on + strand, within Psest_0479 at 530.054 kb on + strand, within Psest_0479 at 530.054 kb on + strand, within Psest_0479 at 530.054 kb on + strand, within Psest_0479 at 530.054 kb on + strand, within Psest_0479 at 530.054 kb on + strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.055 kb on - strand, within Psest_0479 at 530.119 kb on + strand, within Psest_0479 at 530.317 kb on + strand, within Psest_0480 at 530.317 kb on + strand, within Psest_0480 at 530.317 kb on + strand, within Psest_0480 at 530.317 kb on + strand, within Psest_0480 at 530.317 kb on + strand, within Psest_0480 at 530.317 kb on + strand, within Psest_0480 at 530.318 kb on - strand, within Psest_0480 at 530.318 kb on - strand, within Psest_0480 at 530.318 kb on - strand, within Psest_0480 at 530.318 kb on - strand, within Psest_0480 at 530.318 kb on - strand, within Psest_0480 at 530.321 kb on + strand, within Psest_0480 at 530.321 kb on + strand, within Psest_0480 at 530.321 kb on + strand, within Psest_0480 at 530.322 kb on - strand, within Psest_0480 at 530.322 kb on - strand, within Psest_0480 at 530.322 kb on - strand, within Psest_0480
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 527,701 + Psest_0477 0.51 -1.9 527,701 + Psest_0477 0.51 -0.4 527,701 + Psest_0477 0.51 +0.4 527,701 + Psest_0477 0.51 -0.4 527,702 - Psest_0477 0.51 -2.3 527,702 - Psest_0477 0.51 -0.8 527,702 - Psest_0477 0.51 -1.3 527,702 - Psest_0477 0.51 -1.5 527,702 - Psest_0477 0.51 -2.8 527,702 - Psest_0477 0.51 -2.5 527,724 - Psest_0477 0.52 -0.1 527,735 - Psest_0477 0.53 -0.9 527,872 + Psest_0477 0.60 -2.0 528,094 + Psest_0477 0.72 -1.4 528,094 + Psest_0477 0.72 -1.5 528,094 + Psest_0477 0.72 -0.3 528,094 + Psest_0477 0.72 +0.3 528,094 + Psest_0477 0.72 -2.0 528,094 + Psest_0477 0.72 -1.4 528,095 - Psest_0477 0.72 -2.0 528,199 + Psest_0477 0.77 +0.4 528,200 - Psest_0477 0.77 -1.2 528,265 + Psest_0477 0.81 -1.3 528,265 + Psest_0477 0.81 -1.9 528,265 + Psest_0477 0.81 -2.3 528,265 + Psest_0477 0.81 -2.2 528,265 + Psest_0477 0.81 -1.1 528,265 + Psest_0477 0.81 -2.0 528,265 + Psest_0477 0.81 -1.5 528,266 - Psest_0477 0.81 -1.5 528,514 + -1.1 528,515 - -0.3 528,610 + -0.4 528,610 + +2.6 528,610 + -2.1 528,610 + +0.7 528,610 + +1.0 528,611 - -1.0 528,611 - -1.9 528,611 - -0.1 528,611 - -1.3 528,611 - -1.3 528,611 - -1.0 528,611 - -1.1 528,611 - +0.3 528,623 - -1.1 528,690 - +1.0 528,690 - +1.0 528,770 + Psest_0478 0.12 +0.5 528,770 + Psest_0478 0.12 -0.5 528,770 + Psest_0478 0.12 +2.0 528,770 + Psest_0478 0.12 +0.3 528,771 - Psest_0478 0.12 -1.5 528,771 - Psest_0478 0.12 -0.2 528,771 - Psest_0478 0.12 -2.0 528,771 - Psest_0478 0.12 +0.7 528,771 - Psest_0478 0.12 +0.0 528,779 + Psest_0478 0.13 -0.3 528,779 + Psest_0478 0.13 +0.9 528,779 + Psest_0478 0.13 -2.3 528,779 + Psest_0478 0.13 +1.0 528,780 - Psest_0478 0.13 +1.5 528,780 - Psest_0478 0.13 -0.1 528,780 - Psest_0478 0.13 +0.5 528,780 - Psest_0478 0.13 -0.5 528,983 + Psest_0478 0.41 -1.5 528,983 + Psest_0478 0.41 -2.3 528,983 + Psest_0478 0.41 +0.0 528,984 - Psest_0478 0.41 -2.4 528,984 - Psest_0478 0.41 -0.5 529,028 + Psest_0478 0.47 +0.0 529,028 + Psest_0478 0.47 +2.0 529,028 + Psest_0478 0.47 +0.9 529,028 + Psest_0478 0.47 -0.5 529,028 + Psest_0478 0.47 -0.5 529,028 + Psest_0478 0.47 +0.3 529,028 + Psest_0478 0.47 +1.4 529,029 - Psest_0478 0.47 -1.0 529,029 - Psest_0478 0.47 -0.9 529,029 - Psest_0478 0.47 -1.6 529,029 - Psest_0478 0.47 +2.3 529,029 - Psest_0478 0.47 -1.5 529,034 + Psest_0478 0.48 +0.5 529,034 + Psest_0478 0.48 +0.6 529,034 + Psest_0478 0.48 -1.0 529,034 + Psest_0478 0.48 +1.7 529,034 + Psest_0478 0.48 -2.8 529,035 - Psest_0478 0.48 -0.8 529,035 - Psest_0478 0.48 -0.6 529,035 - Psest_0478 0.48 +1.3 529,035 - Psest_0478 0.48 -1.1 529,094 + Psest_0478 0.56 +1.5 529,094 + Psest_0478 0.56 +1.0 529,095 - Psest_0478 0.56 +3.7 529,095 - Psest_0478 0.56 -0.6 529,095 - Psest_0478 0.56 -1.4 529,139 + Psest_0478 0.62 +1.5 529,140 - Psest_0478 0.62 -2.4 529,140 - Psest_0478 0.62 -2.0 529,140 - Psest_0478 0.62 +2.4 529,403 + +2.0 529,403 + +2.5 529,403 + +0.0 529,403 + -0.3 529,403 + +5.0 529,403 + -0.8 529,403 + -0.3 529,403 + +1.9 529,403 + -1.1 529,404 - -0.8 529,404 - +0.7 529,404 - +1.5 529,404 - +1.8 529,404 - -1.6 529,404 - +0.9 529,404 - -1.4 529,782 + Psest_0479 0.23 -1.0 529,803 + Psest_0479 0.27 -1.1 529,804 - Psest_0479 0.27 +0.7 529,804 - Psest_0479 0.27 +1.6 529,804 - Psest_0479 0.27 +1.8 530,012 + Psest_0479 0.61 +0.5 530,012 + Psest_0479 0.61 -2.0 530,048 + Psest_0479 0.67 +1.0 530,048 + Psest_0479 0.67 +1.6 530,048 + Psest_0479 0.67 +0.0 530,048 + Psest_0479 0.67 +1.6 530,048 + Psest_0479 0.67 +3.3 530,048 + Psest_0479 0.67 -2.4 530,049 - Psest_0479 0.67 -0.5 530,049 - Psest_0479 0.67 +1.1 530,049 - Psest_0479 0.67 +4.0 530,049 - Psest_0479 0.67 -1.4 530,049 - Psest_0479 0.67 +0.4 530,049 - Psest_0479 0.67 -1.1 530,054 + Psest_0479 0.67 -0.8 530,054 + Psest_0479 0.67 -1.9 530,054 + Psest_0479 0.67 -1.7 530,054 + Psest_0479 0.67 +0.4 530,054 + Psest_0479 0.67 +1.4 530,054 + Psest_0479 0.67 +1.0 530,054 + Psest_0479 0.67 +1.0 530,055 - Psest_0479 0.68 +4.1 530,055 - Psest_0479 0.68 -0.3 530,055 - Psest_0479 0.68 -0.6 530,055 - Psest_0479 0.68 -2.7 530,055 - Psest_0479 0.68 -0.2 530,055 - Psest_0479 0.68 +0.2 530,055 - Psest_0479 0.68 -1.4 530,055 - Psest_0479 0.68 +0.7 530,055 - Psest_0479 0.68 -1.0 530,055 - Psest_0479 0.68 -1.1 530,055 - Psest_0479 0.68 +6.0 530,055 - Psest_0479 0.68 -0.1 530,055 - Psest_0479 0.68 -2.0 530,055 - Psest_0479 0.68 +0.7 530,119 + Psest_0479 0.78 -0.5 530,317 + Psest_0480 0.15 -2.0 530,317 + Psest_0480 0.15 -2.1 530,317 + Psest_0480 0.15 +0.5 530,317 + Psest_0480 0.15 +2.2 530,317 + Psest_0480 0.15 -0.3 530,317 + Psest_0480 0.15 -1.0 530,318 - Psest_0480 0.15 -2.6 530,318 - Psest_0480 0.15 +0.5 530,318 - Psest_0480 0.15 +0.9 530,318 - Psest_0480 0.15 -4.1 530,318 - Psest_0480 0.15 -2.0 530,321 + Psest_0480 0.16 +0.9 530,321 + Psest_0480 0.16 +1.0 530,321 + Psest_0480 0.16 +3.4 530,322 - Psest_0480 0.16 +0.2 530,322 - Psest_0480 0.16 +2.2 530,322 - Psest_0480 0.16 -1.3
Or see this region's nucleotide sequence