Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0473 and Psest_0474 overlap by 8 nucleotides Psest_0474 and Psest_0475 are separated by 112 nucleotides Psest_0475 and Psest_0476 are separated by 196 nucleotides
Psest_0473: Psest_0473 - Glycerol-3-phosphate dehydrogenase, at 521,763 to 522,938
_0473
Psest_0474: Psest_0474 - Membrane transporters of cations and cationic drugs, at 522,931 to 523,329
_0474
Psest_0475: Psest_0475 - 3-deoxy-D-manno-octulosonic-acid transferase, at 523,442 to 524,710
_0475
Psest_0476: Psest_0476 - type I secretion outer membrane protein, TolC family, at 524,907 to 526,361
_0476
Position (kb) 523
524
525 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 522.952 kb on - strand at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 523.151 kb on + strand, within Psest_0474 at 523.151 kb on + strand, within Psest_0474 at 523.313 kb on + strand at 523.313 kb on + strand at 523.314 kb on - strand at 523.345 kb on - strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand at 524.796 kb on + strand at 524.827 kb on + strand at 524.830 kb on + strand at 524.830 kb on + strand at 524.831 kb on - strand at 524.831 kb on - strand at 524.831 kb on - strand at 524.864 kb on + strand at 524.864 kb on + strand at 524.865 kb on - strand at 524.865 kb on - strand at 524.980 kb on + strand at 524.980 kb on + strand at 524.980 kb on + strand at 525.070 kb on + strand, within Psest_0476 at 525.070 kb on + strand, within Psest_0476 at 525.070 kb on + strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.071 kb on - strand, within Psest_0476 at 525.091 kb on + strand, within Psest_0476 at 525.091 kb on + strand, within Psest_0476 at 525.091 kb on + strand, within Psest_0476 at 525.092 kb on - strand, within Psest_0476 at 525.092 kb on - strand, within Psest_0476 at 525.132 kb on + strand, within Psest_0476 at 525.132 kb on + strand, within Psest_0476 at 525.328 kb on + strand, within Psest_0476 at 525.331 kb on + strand, within Psest_0476 at 525.331 kb on + strand, within Psest_0476 at 525.332 kb on - strand, within Psest_0476 at 525.332 kb on - strand, within Psest_0476 at 525.370 kb on + strand, within Psest_0476 at 525.371 kb on - strand, within Psest_0476 at 525.371 kb on - strand, within Psest_0476 at 525.575 kb on - strand, within Psest_0476 at 525.691 kb on + strand, within Psest_0476 at 525.691 kb on + strand, within Psest_0476 at 525.691 kb on + strand, within Psest_0476 at 525.692 kb on - strand, within Psest_0476 at 525.692 kb on - strand, within Psest_0476
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 522,952 - -0.9 522,973 - Psest_0474 0.11 -2.5 522,973 - Psest_0474 0.11 -1.1 522,973 - Psest_0474 0.11 -0.2 522,973 - Psest_0474 0.11 -1.9 522,973 - Psest_0474 0.11 +1.0 522,974 + Psest_0474 0.11 +1.1 522,974 + Psest_0474 0.11 -2.2 522,974 + Psest_0474 0.11 +0.6 522,974 + Psest_0474 0.11 +0.8 522,974 + Psest_0474 0.11 +0.4 522,975 - Psest_0474 0.11 -1.8 522,975 - Psest_0474 0.11 -0.0 522,975 - Psest_0474 0.11 -2.0 522,975 - Psest_0474 0.11 +1.1 522,975 - Psest_0474 0.11 -1.2 522,975 - Psest_0474 0.11 -0.9 522,975 - Psest_0474 0.11 +2.1 522,975 - Psest_0474 0.11 +2.2 523,151 + Psest_0474 0.55 -1.3 523,151 + Psest_0474 0.55 +1.7 523,313 + -0.2 523,313 + -1.3 523,314 - +2.8 523,345 - +2.3 523,457 + -1.9 523,457 + -1.3 523,457 + -1.3 523,457 + -0.3 523,457 + +0.1 524,796 + -1.8 524,827 + -0.0 524,830 + +1.1 524,830 + -1.6 524,831 - -0.2 524,831 - -2.8 524,831 - +1.0 524,864 + -0.6 524,864 + +1.1 524,865 - -1.7 524,865 - -0.9 524,980 + -0.4 524,980 + -0.8 524,980 + -1.9 525,070 + Psest_0476 0.11 -3.2 525,070 + Psest_0476 0.11 +0.1 525,070 + Psest_0476 0.11 +0.1 525,071 - Psest_0476 0.11 +0.5 525,071 - Psest_0476 0.11 +1.1 525,071 - Psest_0476 0.11 -2.3 525,071 - Psest_0476 0.11 -1.5 525,071 - Psest_0476 0.11 +1.3 525,071 - Psest_0476 0.11 -2.3 525,091 + Psest_0476 0.13 +0.1 525,091 + Psest_0476 0.13 +1.8 525,091 + Psest_0476 0.13 +2.4 525,092 - Psest_0476 0.13 -2.3 525,092 - Psest_0476 0.13 +2.8 525,132 + Psest_0476 0.15 -1.8 525,132 + Psest_0476 0.15 -1.0 525,328 + Psest_0476 0.29 -2.8 525,331 + Psest_0476 0.29 +1.2 525,331 + Psest_0476 0.29 -0.3 525,332 - Psest_0476 0.29 -0.3 525,332 - Psest_0476 0.29 -0.5 525,370 + Psest_0476 0.32 +2.5 525,371 - Psest_0476 0.32 -0.4 525,371 - Psest_0476 0.32 +0.1 525,575 - Psest_0476 0.46 -2.9 525,691 + Psest_0476 0.54 -2.5 525,691 + Psest_0476 0.54 -1.9 525,691 + Psest_0476 0.54 -0.8 525,692 - Psest_0476 0.54 -1.5 525,692 - Psest_0476 0.54 +3.1
Or see this region's nucleotide sequence