Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0404 and Psest_0405 overlap by 4 nucleotides Psest_0405 and Psest_0406 overlap by 41 nucleotides Psest_0406 and Psest_0407 are separated by 83 nucleotides
Psest_0404: Psest_0404 - phytoene desaturase, at 439,233 to 440,738
_0404
Psest_0405: Psest_0405 - Phytoene/squalene synthetase, at 440,735 to 441,661
_0405
Psest_0406: Psest_0406 - Fatty acid hydroxylase superfamily., at 441,621 to 442,154
_0406
Psest_0407: Psest_0407 - Dioxygenases related to 2-nitropropane dioxygenase, at 442,238 to 443,281
_0407
Position (kb) 441
442
443 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.624 kb on + strand at 440.849 kb on + strand, within Psest_0405 at 440.849 kb on + strand, within Psest_0405 at 440.975 kb on + strand, within Psest_0405 at 440.975 kb on + strand, within Psest_0405 at 440.976 kb on - strand, within Psest_0405 at 441.099 kb on - strand, within Psest_0405 at 441.099 kb on - strand, within Psest_0405 at 441.099 kb on - strand, within Psest_0405 at 441.131 kb on + strand, within Psest_0405 at 441.131 kb on + strand, within Psest_0405 at 441.291 kb on - strand, within Psest_0405 at 441.349 kb on - strand, within Psest_0405 at 441.407 kb on + strand, within Psest_0405 at 441.411 kb on - strand, within Psest_0405 at 441.791 kb on + strand, within Psest_0406 at 441.838 kb on + strand, within Psest_0406 at 441.839 kb on - strand, within Psest_0406 at 441.839 kb on - strand, within Psest_0406 at 441.886 kb on + strand, within Psest_0406 at 441.886 kb on + strand, within Psest_0406 at 441.898 kb on + strand, within Psest_0406 at 441.994 kb on + strand, within Psest_0406 at 441.994 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.137 kb on - strand at 442.154 kb on + strand at 442.154 kb on + strand at 442.155 kb on - strand at 442.155 kb on - strand at 442.231 kb on + strand at 442.495 kb on + strand, within Psest_0407 at 442.566 kb on + strand, within Psest_0407 at 442.577 kb on + strand, within Psest_0407 at 442.578 kb on - strand, within Psest_0407 at 442.578 kb on - strand, within Psest_0407 at 442.578 kb on - strand, within Psest_0407 at 442.578 kb on - strand, within Psest_0407 at 442.583 kb on + strand, within Psest_0407 at 442.584 kb on - strand, within Psest_0407 at 443.091 kb on + strand, within Psest_0407 at 443.091 kb on + strand, within Psest_0407 at 443.091 kb on + strand, within Psest_0407 at 443.092 kb on - strand, within Psest_0407
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 440,622 + +0.7 440,622 + -0.7 440,622 + +1.2 440,622 + +2.0 440,622 + +0.7 440,622 + -1.8 440,622 + +1.9 440,622 + -2.4 440,622 + +1.8 440,623 - -1.0 440,623 - -2.1 440,623 - -1.0 440,623 - +0.2 440,623 - +1.0 440,623 - +3.7 440,623 - +0.6 440,623 - -0.1 440,623 - -0.8 440,623 - +0.0 440,623 - +2.2 440,623 - -3.1 440,624 + +2.9 440,849 + Psest_0405 0.12 -2.9 440,849 + Psest_0405 0.12 +0.8 440,975 + Psest_0405 0.26 -1.3 440,975 + Psest_0405 0.26 -0.7 440,976 - Psest_0405 0.26 -1.5 441,099 - Psest_0405 0.39 +0.5 441,099 - Psest_0405 0.39 -1.7 441,099 - Psest_0405 0.39 -1.7 441,131 + Psest_0405 0.43 +1.7 441,131 + Psest_0405 0.43 -2.1 441,291 - Psest_0405 0.60 +1.7 441,349 - Psest_0405 0.66 +0.7 441,407 + Psest_0405 0.72 +0.7 441,411 - Psest_0405 0.73 -1.9 441,791 + Psest_0406 0.32 -1.4 441,838 + Psest_0406 0.41 -0.3 441,839 - Psest_0406 0.41 +2.0 441,839 - Psest_0406 0.41 -0.7 441,886 + Psest_0406 0.50 -0.2 441,886 + Psest_0406 0.50 -0.3 441,898 + Psest_0406 0.52 -0.4 441,994 + Psest_0406 0.70 -0.7 441,994 + Psest_0406 0.70 -1.0 442,075 + Psest_0406 0.85 -2.0 442,075 + Psest_0406 0.85 -2.3 442,075 + Psest_0406 0.85 +1.6 442,075 + Psest_0406 0.85 -2.7 442,075 + Psest_0406 0.85 +1.3 442,075 + Psest_0406 0.85 +1.0 442,075 + Psest_0406 0.85 -1.5 442,075 + Psest_0406 0.85 -0.4 442,075 + Psest_0406 0.85 +3.0 442,075 + Psest_0406 0.85 -0.5 442,075 + Psest_0406 0.85 +1.4 442,075 + Psest_0406 0.85 +0.1 442,075 + Psest_0406 0.85 -1.0 442,076 - Psest_0406 0.85 +1.0 442,076 - Psest_0406 0.85 +2.1 442,076 - Psest_0406 0.85 +0.1 442,076 - Psest_0406 0.85 -1.9 442,076 - Psest_0406 0.85 +2.8 442,076 - Psest_0406 0.85 -1.3 442,076 - Psest_0406 0.85 -1.6 442,076 - Psest_0406 0.85 -0.4 442,076 - Psest_0406 0.85 -1.1 442,076 - Psest_0406 0.85 -2.3 442,076 - Psest_0406 0.85 -0.4 442,076 - Psest_0406 0.85 -3.9 442,076 - Psest_0406 0.85 +0.7 442,137 - -1.5 442,154 + -1.9 442,154 + +0.0 442,155 - +1.0 442,155 - -1.7 442,231 + -0.4 442,495 + Psest_0407 0.25 -0.4 442,566 + Psest_0407 0.31 -3.7 442,577 + Psest_0407 0.32 -1.3 442,578 - Psest_0407 0.33 +2.0 442,578 - Psest_0407 0.33 +1.3 442,578 - Psest_0407 0.33 +2.2 442,578 - Psest_0407 0.33 +0.9 442,583 + Psest_0407 0.33 -1.0 442,584 - Psest_0407 0.33 -3.5 443,091 + Psest_0407 0.82 -2.9 443,091 + Psest_0407 0.82 +0.0 443,091 + Psest_0407 0.82 -2.2 443,092 - Psest_0407 0.82 -1.7
Or see this region's nucleotide sequence