Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3538 and Psest_3539 are separated by 10 nucleotides Psest_3539 and Psest_3540 overlap by 4 nucleotides
Psest_3538: Psest_3538 - hypothetical protein, at 3,715,233 to 3,716,282
_3538
Psest_3539: Psest_3539 - exonuclease SbcD, at 3,716,293 to 3,717,528
_3539
Psest_3540: Psest_3540 - hypothetical protein, at 3,717,525 to 3,721,178
_3540
Position (kb)
3716
3717
3718 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3715.299 kb on + strand at 3715.328 kb on + strand at 3715.328 kb on + strand at 3715.328 kb on + strand at 3715.328 kb on + strand at 3715.328 kb on + strand at 3715.329 kb on - strand at 3715.329 kb on - strand at 3715.385 kb on + strand, within Psest_3538 at 3715.470 kb on - strand, within Psest_3538 at 3715.470 kb on - strand, within Psest_3538 at 3715.470 kb on - strand, within Psest_3538 at 3715.756 kb on + strand, within Psest_3538 at 3715.757 kb on - strand, within Psest_3538 at 3715.757 kb on - strand, within Psest_3538 at 3715.780 kb on + strand, within Psest_3538 at 3715.780 kb on + strand, within Psest_3538 at 3715.780 kb on + strand, within Psest_3538 at 3715.780 kb on + strand, within Psest_3538 at 3715.781 kb on - strand, within Psest_3538 at 3715.781 kb on - strand, within Psest_3538 at 3715.781 kb on - strand, within Psest_3538 at 3715.781 kb on - strand, within Psest_3538 at 3715.781 kb on - strand, within Psest_3538 at 3715.868 kb on + strand, within Psest_3538 at 3715.868 kb on + strand, within Psest_3538 at 3715.868 kb on + strand, within Psest_3538 at 3715.868 kb on + strand, within Psest_3538 at 3715.868 kb on + strand, within Psest_3538 at 3715.869 kb on - strand, within Psest_3538 at 3716.130 kb on - strand, within Psest_3538 at 3716.179 kb on + strand at 3716.179 kb on + strand at 3716.180 kb on - strand at 3716.180 kb on - strand at 3716.180 kb on - strand at 3716.215 kb on + strand at 3716.215 kb on + strand at 3716.215 kb on + strand at 3716.215 kb on + strand at 3716.215 kb on + strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.620 kb on - strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.959 kb on + strand, within Psest_3539 at 3716.960 kb on - strand, within Psest_3539 at 3716.960 kb on - strand, within Psest_3539 at 3716.960 kb on - strand, within Psest_3539 at 3717.245 kb on + strand, within Psest_3539 at 3717.246 kb on - strand, within Psest_3539 at 3717.377 kb on + strand, within Psest_3539 at 3717.377 kb on + strand, within Psest_3539 at 3717.378 kb on - strand, within Psest_3539 at 3717.378 kb on - strand, within Psest_3539 at 3717.378 kb on - strand, within Psest_3539 at 3717.378 kb on - strand, within Psest_3539 at 3717.378 kb on - strand, within Psest_3539 at 3717.378 kb on - strand, within Psest_3539 at 3718.117 kb on - strand, within Psest_3540 at 3718.231 kb on + strand, within Psest_3540 at 3718.231 kb on + strand, within Psest_3540 at 3718.231 kb on + strand, within Psest_3540 at 3718.232 kb on - strand, within Psest_3540 at 3718.232 kb on - strand, within Psest_3540
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 3,715,299 + +2.2 3,715,328 + +2.2 3,715,328 + -3.2 3,715,328 + +1.6 3,715,328 + +2.1 3,715,328 + +2.2 3,715,329 - -2.1 3,715,329 - -2.0 3,715,385 + Psest_3538 0.14 -2.7 3,715,470 - Psest_3538 0.23 +0.1 3,715,470 - Psest_3538 0.23 -2.3 3,715,470 - Psest_3538 0.23 -0.2 3,715,756 + Psest_3538 0.50 -2.2 3,715,757 - Psest_3538 0.50 -1.1 3,715,757 - Psest_3538 0.50 +1.2 3,715,780 + Psest_3538 0.52 +0.8 3,715,780 + Psest_3538 0.52 +0.6 3,715,780 + Psest_3538 0.52 +0.3 3,715,780 + Psest_3538 0.52 -2.4 3,715,781 - Psest_3538 0.52 -1.0 3,715,781 - Psest_3538 0.52 -0.5 3,715,781 - Psest_3538 0.52 -1.4 3,715,781 - Psest_3538 0.52 +2.2 3,715,781 - Psest_3538 0.52 -0.7 3,715,868 + Psest_3538 0.60 +0.1 3,715,868 + Psest_3538 0.60 +3.5 3,715,868 + Psest_3538 0.60 +4.1 3,715,868 + Psest_3538 0.60 -1.1 3,715,868 + Psest_3538 0.60 -0.6 3,715,869 - Psest_3538 0.61 -0.2 3,716,130 - Psest_3538 0.85 +0.1 3,716,179 + -1.4 3,716,179 + +1.1 3,716,180 - +2.6 3,716,180 - -1.7 3,716,180 - -2.3 3,716,215 + -0.5 3,716,215 + -2.9 3,716,215 + -3.7 3,716,215 + -0.6 3,716,215 + -0.5 3,716,216 - +2.8 3,716,216 - -2.7 3,716,216 - +1.4 3,716,216 - +0.6 3,716,216 - +3.2 3,716,216 - -1.0 3,716,216 - +1.6 3,716,216 - -4.0 3,716,216 - +2.1 3,716,216 - -0.3 3,716,216 - +1.9 3,716,620 - Psest_3539 0.26 -0.6 3,716,764 + Psest_3539 0.38 -0.5 3,716,764 + Psest_3539 0.38 -0.4 3,716,764 + Psest_3539 0.38 -0.3 3,716,764 + Psest_3539 0.38 +0.3 3,716,764 + Psest_3539 0.38 -1.2 3,716,764 + Psest_3539 0.38 +0.3 3,716,765 - Psest_3539 0.38 -2.2 3,716,765 - Psest_3539 0.38 -3.4 3,716,765 - Psest_3539 0.38 +0.3 3,716,765 - Psest_3539 0.38 -1.8 3,716,765 - Psest_3539 0.38 -1.6 3,716,765 - Psest_3539 0.38 -1.9 3,716,765 - Psest_3539 0.38 -1.9 3,716,765 - Psest_3539 0.38 +0.2 3,716,765 - Psest_3539 0.38 -0.4 3,716,765 - Psest_3539 0.38 -0.8 3,716,765 - Psest_3539 0.38 +0.5 3,716,959 + Psest_3539 0.54 -0.4 3,716,960 - Psest_3539 0.54 -1.5 3,716,960 - Psest_3539 0.54 -1.7 3,716,960 - Psest_3539 0.54 -1.2 3,717,245 + Psest_3539 0.77 -0.6 3,717,246 - Psest_3539 0.77 -0.6 3,717,377 + Psest_3539 0.88 -0.2 3,717,377 + Psest_3539 0.88 -0.4 3,717,378 - Psest_3539 0.88 -1.0 3,717,378 - Psest_3539 0.88 -0.6 3,717,378 - Psest_3539 0.88 -1.4 3,717,378 - Psest_3539 0.88 -1.3 3,717,378 - Psest_3539 0.88 +1.0 3,717,378 - Psest_3539 0.88 +0.8 3,718,117 - Psest_3540 0.16 -1.2 3,718,231 + Psest_3540 0.19 +1.7 3,718,231 + Psest_3540 0.19 +1.8 3,718,231 + Psest_3540 0.19 +3.2 3,718,232 - Psest_3540 0.19 -0.6 3,718,232 - Psest_3540 0.19 -0.6
Or see this region's nucleotide sequence