Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3019 and Psest_3020 are separated by 76 nucleotides Psest_3020 and Psest_3021 are separated by 67 nucleotides Psest_3021 and Psest_3022 are separated by 134 nucleotides Psest_3022 and Psest_3023 are separated by 5 nucleotides Psest_3023 and Psest_3024 are separated by 48 nucleotides
Psest_3019: Psest_3019 - Cell wall-associated hydrolases (invasion-associated proteins), at 3,208,150 to 3,208,674
_3019
Psest_3020: Psest_3020 - Cell wall-associated hydrolases (invasion-associated proteins), at 3,208,751 to 3,209,383
_3020
Psest_3021: Psest_3021 - NAD-dependent protein deacetylases, SIR2 family, at 3,209,451 to 3,210,215
_3021
Psest_3022: Psest_3022 - Predicted membrane protein, at 3,210,350 to 3,210,640
_3022
Psest_3023: Psest_3023 - cyd operon protein YbgT, at 3,210,646 to 3,210,750
_3023
Psest_3024: Psest_3024 - cytochrome d oxidase, subunit II (cydB), at 3,210,799 to 3,211,941
_3024
Position (kb)
3209
3210
3211 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3208.488 kb on + strand, within Psest_3019 at 3208.488 kb on + strand, within Psest_3019 at 3208.489 kb on - strand, within Psest_3019 at 3208.668 kb on - strand at 3208.815 kb on + strand, within Psest_3020 at 3208.815 kb on + strand, within Psest_3020 at 3208.816 kb on - strand, within Psest_3020 at 3208.816 kb on - strand, within Psest_3020 at 3208.816 kb on - strand, within Psest_3020 at 3208.816 kb on - strand, within Psest_3020 at 3208.816 kb on - strand, within Psest_3020 at 3208.830 kb on + strand, within Psest_3020 at 3208.830 kb on + strand, within Psest_3020 at 3208.831 kb on - strand, within Psest_3020 at 3208.831 kb on - strand, within Psest_3020 at 3208.831 kb on - strand, within Psest_3020 at 3208.908 kb on + strand, within Psest_3020 at 3208.908 kb on + strand, within Psest_3020 at 3208.908 kb on + strand, within Psest_3020 at 3208.908 kb on + strand, within Psest_3020 at 3208.908 kb on + strand, within Psest_3020 at 3208.909 kb on - strand, within Psest_3020 at 3208.909 kb on - strand, within Psest_3020 at 3208.909 kb on - strand, within Psest_3020 at 3208.909 kb on - strand, within Psest_3020 at 3208.909 kb on - strand, within Psest_3020 at 3209.310 kb on - strand, within Psest_3020 at 3209.345 kb on - strand at 3209.475 kb on + strand at 3209.475 kb on + strand at 3209.475 kb on + strand at 3209.475 kb on + strand at 3209.476 kb on - strand at 3209.553 kb on + strand, within Psest_3021 at 3209.553 kb on + strand, within Psest_3021 at 3209.635 kb on - strand, within Psest_3021 at 3210.010 kb on + strand, within Psest_3021 at 3210.010 kb on + strand, within Psest_3021 at 3210.010 kb on + strand, within Psest_3021 at 3210.011 kb on - strand, within Psest_3021 at 3210.097 kb on + strand, within Psest_3021 at 3210.098 kb on - strand, within Psest_3021 at 3210.098 kb on - strand, within Psest_3021 at 3210.098 kb on - strand, within Psest_3021 at 3210.098 kb on - strand, within Psest_3021 at 3210.119 kb on - strand, within Psest_3021 at 3210.210 kb on - strand at 3210.304 kb on + strand at 3210.351 kb on + strand at 3210.351 kb on + strand at 3210.351 kb on + strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.352 kb on - strand at 3210.470 kb on + strand, within Psest_3022 at 3210.470 kb on + strand, within Psest_3022 at 3210.470 kb on + strand, within Psest_3022 at 3210.471 kb on - strand, within Psest_3022 at 3210.471 kb on - strand, within Psest_3022 at 3210.471 kb on - strand, within Psest_3022 at 3210.471 kb on - strand, within Psest_3022 at 3210.522 kb on + strand, within Psest_3022 at 3210.523 kb on - strand, within Psest_3022 at 3210.743 kb on + strand at 3210.744 kb on - strand at 3210.744 kb on - strand at 3210.744 kb on - strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.750 kb on + strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.751 kb on - strand at 3210.803 kb on + strand at 3210.804 kb on - strand at 3210.804 kb on - strand at 3210.863 kb on + strand at 3210.864 kb on - strand at 3210.864 kb on - strand at 3210.864 kb on - strand at 3210.864 kb on - strand at 3210.864 kb on - strand at 3210.879 kb on - strand at 3210.940 kb on - strand, within Psest_3024 at 3210.940 kb on - strand, within Psest_3024 at 3211.163 kb on + strand, within Psest_3024 at 3211.163 kb on + strand, within Psest_3024 at 3211.163 kb on + strand, within Psest_3024 at 3211.163 kb on + strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024 at 3211.164 kb on - strand, within Psest_3024
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 3,208,488 + Psest_3019 0.64 -0.1 3,208,488 + Psest_3019 0.64 +0.0 3,208,489 - Psest_3019 0.65 +2.0 3,208,668 - -1.2 3,208,815 + Psest_3020 0.10 -0.3 3,208,815 + Psest_3020 0.10 -1.6 3,208,816 - Psest_3020 0.10 -2.2 3,208,816 - Psest_3020 0.10 +1.4 3,208,816 - Psest_3020 0.10 -1.7 3,208,816 - Psest_3020 0.10 +0.9 3,208,816 - Psest_3020 0.10 -2.9 3,208,830 + Psest_3020 0.12 -0.3 3,208,830 + Psest_3020 0.12 +1.0 3,208,831 - Psest_3020 0.13 -0.0 3,208,831 - Psest_3020 0.13 -1.9 3,208,831 - Psest_3020 0.13 +2.6 3,208,908 + Psest_3020 0.25 -0.6 3,208,908 + Psest_3020 0.25 -2.2 3,208,908 + Psest_3020 0.25 -1.2 3,208,908 + Psest_3020 0.25 +0.9 3,208,908 + Psest_3020 0.25 -0.1 3,208,909 - Psest_3020 0.25 +3.1 3,208,909 - Psest_3020 0.25 -0.1 3,208,909 - Psest_3020 0.25 -1.6 3,208,909 - Psest_3020 0.25 -2.4 3,208,909 - Psest_3020 0.25 -0.0 3,209,310 - Psest_3020 0.88 +3.6 3,209,345 - -0.9 3,209,475 + +0.5 3,209,475 + -0.1 3,209,475 + -1.8 3,209,475 + -1.0 3,209,476 - +3.1 3,209,553 + Psest_3021 0.13 +1.0 3,209,553 + Psest_3021 0.13 -0.1 3,209,635 - Psest_3021 0.24 -1.2 3,210,010 + Psest_3021 0.73 +1.7 3,210,010 + Psest_3021 0.73 +1.1 3,210,010 + Psest_3021 0.73 +1.2 3,210,011 - Psest_3021 0.73 -2.7 3,210,097 + Psest_3021 0.84 +5.5 3,210,098 - Psest_3021 0.85 -0.6 3,210,098 - Psest_3021 0.85 +1.3 3,210,098 - Psest_3021 0.85 -0.1 3,210,098 - Psest_3021 0.85 +1.4 3,210,119 - Psest_3021 0.87 -0.9 3,210,210 - +1.5 3,210,304 + -0.8 3,210,351 + +0.5 3,210,351 + -0.3 3,210,351 + -2.1 3,210,352 - -1.1 3,210,352 - -1.5 3,210,352 - +3.0 3,210,352 - +1.8 3,210,352 - -1.0 3,210,352 - -2.5 3,210,352 - -0.8 3,210,352 - -2.0 3,210,470 + Psest_3022 0.41 -1.8 3,210,470 + Psest_3022 0.41 +1.6 3,210,470 + Psest_3022 0.41 -0.0 3,210,471 - Psest_3022 0.42 -0.6 3,210,471 - Psest_3022 0.42 -0.9 3,210,471 - Psest_3022 0.42 -3.0 3,210,471 - Psest_3022 0.42 +1.7 3,210,522 + Psest_3022 0.59 +0.5 3,210,523 - Psest_3022 0.59 -0.2 3,210,743 + -0.0 3,210,744 - +2.1 3,210,744 - +1.1 3,210,744 - -1.5 3,210,750 + -1.3 3,210,750 + -0.1 3,210,750 + -1.6 3,210,750 + -1.3 3,210,750 + -2.1 3,210,750 + +2.2 3,210,750 + -1.9 3,210,751 - -2.1 3,210,751 - +2.6 3,210,751 - -1.7 3,210,751 - +0.6 3,210,751 - +0.8 3,210,751 - +0.2 3,210,751 - -1.6 3,210,751 - -1.9 3,210,751 - -0.3 3,210,751 - -0.1 3,210,751 - -1.2 3,210,751 - -1.7 3,210,751 - +1.8 3,210,751 - +1.3 3,210,751 - +1.4 3,210,751 - -0.6 3,210,751 - +0.4 3,210,751 - -3.3 3,210,751 - -1.9 3,210,803 + +0.2 3,210,804 - -2.5 3,210,804 - -1.2 3,210,863 + -3.6 3,210,864 - -0.2 3,210,864 - -1.7 3,210,864 - -0.7 3,210,864 - -1.8 3,210,864 - -0.3 3,210,879 - +2.0 3,210,940 - Psest_3024 0.12 -2.7 3,210,940 - Psest_3024 0.12 -0.6 3,211,163 + Psest_3024 0.32 -3.2 3,211,163 + Psest_3024 0.32 +0.6 3,211,163 + Psest_3024 0.32 -0.3 3,211,163 + Psest_3024 0.32 -1.2 3,211,164 - Psest_3024 0.32 +0.1 3,211,164 - Psest_3024 0.32 -0.1 3,211,164 - Psest_3024 0.32 -1.8 3,211,164 - Psest_3024 0.32 -2.4 3,211,164 - Psest_3024 0.32 +1.6 3,211,164 - Psest_3024 0.32 -1.3
Or see this region's nucleotide sequence