Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2474

Experiment: RCH2 grown on a colony of 8R2A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2473 and Psest_2474 are separated by 97 nucleotidesPsest_2474 and Psest_2475 are separated by 174 nucleotides Psest_2473: Psest_2473 - 3-oxoacyl-(acyl-carrier-protein) synthase, at 2,642,426 to 2,643,643 _2473 Psest_2474: Psest_2474 - AraC-type DNA-binding domain-containing proteins, at 2,643,741 to 2,644,784 _2474 Psest_2475: Psest_2475 - Acyl-CoA dehydrogenases, at 2,644,959 to 2,646,137 _2475 Position (kb) 2643 2644 2645Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2644.024 kb on + strand, within Psest_2474at 2644.024 kb on + strand, within Psest_2474at 2644.025 kb on - strand, within Psest_2474at 2644.119 kb on + strand, within Psest_2474at 2644.172 kb on - strand, within Psest_2474at 2644.172 kb on - strand, within Psest_2474at 2644.172 kb on - strand, within Psest_2474at 2644.172 kb on - strand, within Psest_2474at 2644.172 kb on - strand, within Psest_2474at 2644.225 kb on + strand, within Psest_2474at 2644.450 kb on + strand, within Psest_2474at 2644.451 kb on - strand, within Psest_2474at 2644.451 kb on - strand, within Psest_2474at 2644.451 kb on - strand, within Psest_2474at 2644.729 kb on + strandat 2644.729 kb on + strandat 2644.899 kb on - strandat 2644.902 kb on - strandat 2644.902 kb on - strandat 2644.902 kb on - strandat 2644.902 kb on - strandat 2645.213 kb on - strand, within Psest_2475at 2645.213 kb on - strand, within Psest_2475at 2645.277 kb on + strand, within Psest_2475at 2645.277 kb on + strand, within Psest_2475at 2645.277 kb on + strand, within Psest_2475at 2645.452 kb on + strand, within Psest_2475at 2645.452 kb on + strand, within Psest_2475at 2645.688 kb on + strand, within Psest_2475at 2645.778 kb on + strand, within Psest_2475at 2645.779 kb on - strand, within Psest_2475at 2645.779 kb on - strand, within Psest_2475at 2645.779 kb on - strand, within Psest_2475at 2645.779 kb on - strand, within Psest_2475

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A
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2,644,024 + Psest_2474 0.27 -2.2
2,644,024 + Psest_2474 0.27 +0.5
2,644,025 - Psest_2474 0.27 -1.0
2,644,119 + Psest_2474 0.36 +4.1
2,644,172 - Psest_2474 0.41 -1.6
2,644,172 - Psest_2474 0.41 -1.3
2,644,172 - Psest_2474 0.41 -1.4
2,644,172 - Psest_2474 0.41 -0.4
2,644,172 - Psest_2474 0.41 +1.2
2,644,225 + Psest_2474 0.46 +0.2
2,644,450 + Psest_2474 0.68 -2.1
2,644,451 - Psest_2474 0.68 -3.2
2,644,451 - Psest_2474 0.68 -0.6
2,644,451 - Psest_2474 0.68 -0.4
2,644,729 + -1.0
2,644,729 + +2.3
2,644,899 - -0.5
2,644,902 - +2.1
2,644,902 - +0.1
2,644,902 - +2.7
2,644,902 - +1.6
2,645,213 - Psest_2475 0.22 +0.6
2,645,213 - Psest_2475 0.22 -2.5
2,645,277 + Psest_2475 0.27 -1.5
2,645,277 + Psest_2475 0.27 -1.5
2,645,277 + Psest_2475 0.27 +1.4
2,645,452 + Psest_2475 0.42 +0.8
2,645,452 + Psest_2475 0.42 -0.2
2,645,688 + Psest_2475 0.62 -1.4
2,645,778 + Psest_2475 0.69 -2.5
2,645,779 - Psest_2475 0.70 +0.1
2,645,779 - Psest_2475 0.70 -1.9
2,645,779 - Psest_2475 0.70 +0.0
2,645,779 - Psest_2475 0.70 +0.4

Or see this region's nucleotide sequence