Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2448 and Psest_2449 are separated by 71 nucleotides Psest_2449 and Psest_2450 are separated by 239 nucleotides Psest_2450 and Psest_2451 are separated by 261 nucleotides
Psest_2448: Psest_2448 - AraC-type DNA-binding domain-containing proteins, at 2,617,175 to 2,618,242
_2448
Psest_2449: Psest_2449 - Predicted membrane protein, at 2,618,314 to 2,618,751
_2449
Psest_2450: Psest_2450 - hypothetical protein, at 2,618,991 to 2,619,446
_2450
Psest_2451: Psest_2451 - Glutaredoxin and related proteins, at 2,619,708 to 2,620,814
_2451
Position (kb)
2618
2619 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2617.526 kb on + strand, within Psest_2448 at 2617.526 kb on + strand, within Psest_2448 at 2617.527 kb on - strand, within Psest_2448 at 2617.680 kb on + strand, within Psest_2448 at 2617.681 kb on - strand, within Psest_2448 at 2617.681 kb on - strand, within Psest_2448 at 2617.779 kb on + strand, within Psest_2448 at 2617.779 kb on + strand, within Psest_2448 at 2617.779 kb on + strand, within Psest_2448 at 2617.780 kb on - strand, within Psest_2448 at 2618.040 kb on + strand at 2618.351 kb on + strand at 2618.352 kb on - strand at 2618.562 kb on - strand, within Psest_2449 at 2618.562 kb on - strand, within Psest_2449 at 2618.637 kb on + strand, within Psest_2449 at 2618.637 kb on + strand, within Psest_2449 at 2618.638 kb on - strand, within Psest_2449 at 2618.638 kb on - strand, within Psest_2449 at 2618.638 kb on - strand, within Psest_2449 at 2618.638 kb on - strand, within Psest_2449 at 2618.638 kb on - strand, within Psest_2449 at 2618.638 kb on - strand, within Psest_2449 at 2618.639 kb on + strand, within Psest_2449 at 2618.689 kb on + strand, within Psest_2449 at 2618.692 kb on + strand, within Psest_2449 at 2618.792 kb on + strand at 2618.793 kb on - strand at 2618.992 kb on + strand at 2618.993 kb on - strand at 2618.993 kb on - strand at 2619.149 kb on + strand, within Psest_2450 at 2619.184 kb on + strand, within Psest_2450 at 2619.230 kb on - strand, within Psest_2450 at 2619.235 kb on + strand, within Psest_2450 at 2619.236 kb on - strand, within Psest_2450 at 2619.236 kb on - strand, within Psest_2450 at 2619.236 kb on - strand, within Psest_2450 at 2619.236 kb on - strand, within Psest_2450 at 2619.320 kb on + strand, within Psest_2450 at 2619.322 kb on + strand, within Psest_2450 at 2619.322 kb on + strand, within Psest_2450 at 2619.322 kb on + strand, within Psest_2450 at 2619.322 kb on + strand, within Psest_2450 at 2619.322 kb on + strand, within Psest_2450 at 2619.322 kb on + strand, within Psest_2450 at 2619.323 kb on - strand, within Psest_2450 at 2619.323 kb on - strand, within Psest_2450 at 2619.323 kb on - strand, within Psest_2450 at 2619.556 kb on + strand at 2619.556 kb on + strand at 2619.602 kb on + strand at 2619.602 kb on + strand at 2619.602 kb on + strand at 2619.711 kb on + strand at 2619.711 kb on + strand at 2619.711 kb on + strand at 2619.711 kb on + strand at 2619.712 kb on - strand at 2619.712 kb on - strand at 2619.712 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 2,617,526 + Psest_2448 0.33 -0.9 2,617,526 + Psest_2448 0.33 -2.0 2,617,527 - Psest_2448 0.33 -1.5 2,617,680 + Psest_2448 0.47 +1.3 2,617,681 - Psest_2448 0.47 +1.8 2,617,681 - Psest_2448 0.47 -2.9 2,617,779 + Psest_2448 0.57 -1.9 2,617,779 + Psest_2448 0.57 +0.9 2,617,779 + Psest_2448 0.57 +0.4 2,617,780 - Psest_2448 0.57 -0.5 2,618,040 + +1.0 2,618,351 + -0.5 2,618,352 - -1.2 2,618,562 - Psest_2449 0.57 -1.7 2,618,562 - Psest_2449 0.57 +2.1 2,618,637 + Psest_2449 0.74 +1.3 2,618,637 + Psest_2449 0.74 +1.1 2,618,638 - Psest_2449 0.74 -2.6 2,618,638 - Psest_2449 0.74 +1.1 2,618,638 - Psest_2449 0.74 +0.4 2,618,638 - Psest_2449 0.74 +2.0 2,618,638 - Psest_2449 0.74 +2.1 2,618,638 - Psest_2449 0.74 +0.9 2,618,639 + Psest_2449 0.74 +1.2 2,618,689 + Psest_2449 0.86 +2.2 2,618,692 + Psest_2449 0.86 -1.6 2,618,792 + +2.4 2,618,793 - -0.8 2,618,992 + -0.8 2,618,993 - +0.9 2,618,993 - -1.1 2,619,149 + Psest_2450 0.35 -1.0 2,619,184 + Psest_2450 0.42 +1.4 2,619,230 - Psest_2450 0.52 +1.6 2,619,235 + Psest_2450 0.54 -1.3 2,619,236 - Psest_2450 0.54 +1.1 2,619,236 - Psest_2450 0.54 -0.3 2,619,236 - Psest_2450 0.54 +3.2 2,619,236 - Psest_2450 0.54 +1.0 2,619,320 + Psest_2450 0.72 -0.9 2,619,322 + Psest_2450 0.73 +0.8 2,619,322 + Psest_2450 0.73 -0.6 2,619,322 + Psest_2450 0.73 -2.5 2,619,322 + Psest_2450 0.73 -1.0 2,619,322 + Psest_2450 0.73 -0.0 2,619,322 + Psest_2450 0.73 +1.8 2,619,323 - Psest_2450 0.73 +0.9 2,619,323 - Psest_2450 0.73 -0.6 2,619,323 - Psest_2450 0.73 -1.8 2,619,556 + -0.8 2,619,556 + -1.5 2,619,602 + -1.5 2,619,602 + +1.2 2,619,602 + +5.7 2,619,711 + +1.4 2,619,711 + +2.5 2,619,711 + +0.7 2,619,711 + -0.5 2,619,712 - -3.0 2,619,712 - -0.5 2,619,712 - -0.7
Or see this region's nucleotide sequence