Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2409 and Psest_2410 are separated by 3 nucleotides Psest_2410 and Psest_2411 are separated by 207 nucleotides
Psest_2409: Psest_2409 - Serine/threonine protein phosphatase, at 2,564,194 to 2,565,945
_2409
Psest_2410: Psest_2410 - Superfamily I DNA and RNA helicases, at 2,565,949 to 2,567,919
_2410
Psest_2411: Psest_2411 - PAS domain S-box, at 2,568,127 to 2,571,120
_2411
Position (kb)
2565
2566
2567
2568 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2565.253 kb on + strand, within Psest_2409 at 2565.259 kb on + strand, within Psest_2409 at 2565.259 kb on + strand, within Psest_2409 at 2565.260 kb on - strand, within Psest_2409 at 2565.260 kb on - strand, within Psest_2409 at 2565.260 kb on - strand, within Psest_2409 at 2565.542 kb on - strand, within Psest_2409 at 2565.544 kb on - strand, within Psest_2409 at 2565.556 kb on + strand, within Psest_2409 at 2565.595 kb on + strand, within Psest_2409 at 2565.596 kb on - strand, within Psest_2409 at 2566.076 kb on + strand at 2566.077 kb on - strand at 2566.077 kb on - strand at 2566.077 kb on - strand at 2566.106 kb on + strand at 2566.106 kb on + strand at 2566.200 kb on + strand, within Psest_2410 at 2566.200 kb on + strand, within Psest_2410 at 2566.201 kb on - strand, within Psest_2410 at 2566.202 kb on + strand, within Psest_2410 at 2566.202 kb on + strand, within Psest_2410 at 2566.202 kb on + strand, within Psest_2410 at 2566.203 kb on - strand, within Psest_2410 at 2566.390 kb on + strand, within Psest_2410 at 2566.559 kb on + strand, within Psest_2410 at 2566.559 kb on + strand, within Psest_2410 at 2566.560 kb on - strand, within Psest_2410 at 2566.860 kb on - strand, within Psest_2410 at 2566.979 kb on + strand, within Psest_2410 at 2566.979 kb on + strand, within Psest_2410 at 2566.979 kb on + strand, within Psest_2410 at 2566.980 kb on - strand, within Psest_2410 at 2566.980 kb on - strand, within Psest_2410 at 2566.980 kb on - strand, within Psest_2410 at 2566.980 kb on - strand, within Psest_2410 at 2566.980 kb on - strand, within Psest_2410 at 2566.991 kb on + strand, within Psest_2410 at 2566.992 kb on - strand, within Psest_2410 at 2567.141 kb on + strand, within Psest_2410 at 2567.153 kb on + strand, within Psest_2410 at 2567.154 kb on - strand, within Psest_2410 at 2567.663 kb on + strand, within Psest_2410 at 2567.663 kb on + strand, within Psest_2410 at 2567.663 kb on + strand, within Psest_2410 at 2567.664 kb on - strand, within Psest_2410 at 2567.664 kb on - strand, within Psest_2410 at 2567.853 kb on + strand at 2567.854 kb on - strand at 2568.170 kb on + strand at 2568.170 kb on + strand at 2568.170 kb on + strand at 2568.170 kb on + strand at 2568.170 kb on + strand at 2568.170 kb on + strand at 2568.170 kb on + strand at 2568.170 kb on + strand at 2568.170 kb on + strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.171 kb on - strand at 2568.343 kb on + strand at 2568.343 kb on + strand at 2568.343 kb on + strand at 2568.343 kb on + strand at 2568.742 kb on + strand, within Psest_2411 at 2568.743 kb on - strand, within Psest_2411
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 2,565,253 + Psest_2409 0.60 -0.5 2,565,259 + Psest_2409 0.61 +1.4 2,565,259 + Psest_2409 0.61 -0.9 2,565,260 - Psest_2409 0.61 -1.5 2,565,260 - Psest_2409 0.61 +2.4 2,565,260 - Psest_2409 0.61 +1.3 2,565,542 - Psest_2409 0.77 +1.0 2,565,544 - Psest_2409 0.77 -0.6 2,565,556 + Psest_2409 0.78 +1.3 2,565,595 + Psest_2409 0.80 +0.9 2,565,596 - Psest_2409 0.80 +2.2 2,566,076 + -1.7 2,566,077 - +3.4 2,566,077 - -1.2 2,566,077 - +0.4 2,566,106 + +0.2 2,566,106 + +2.4 2,566,200 + Psest_2410 0.13 -0.6 2,566,200 + Psest_2410 0.13 -0.6 2,566,201 - Psest_2410 0.13 +0.8 2,566,202 + Psest_2410 0.13 +0.1 2,566,202 + Psest_2410 0.13 +2.4 2,566,202 + Psest_2410 0.13 +0.8 2,566,203 - Psest_2410 0.13 -1.2 2,566,390 + Psest_2410 0.22 +1.3 2,566,559 + Psest_2410 0.31 +1.0 2,566,559 + Psest_2410 0.31 +0.4 2,566,560 - Psest_2410 0.31 -1.0 2,566,860 - Psest_2410 0.46 -0.5 2,566,979 + Psest_2410 0.52 -2.3 2,566,979 + Psest_2410 0.52 -2.0 2,566,979 + Psest_2410 0.52 +4.3 2,566,980 - Psest_2410 0.52 -3.0 2,566,980 - Psest_2410 0.52 +1.6 2,566,980 - Psest_2410 0.52 +5.1 2,566,980 - Psest_2410 0.52 +0.3 2,566,980 - Psest_2410 0.52 +0.9 2,566,991 + Psest_2410 0.53 +1.0 2,566,992 - Psest_2410 0.53 +1.4 2,567,141 + Psest_2410 0.60 +1.7 2,567,153 + Psest_2410 0.61 +0.0 2,567,154 - Psest_2410 0.61 -0.1 2,567,663 + Psest_2410 0.87 +2.4 2,567,663 + Psest_2410 0.87 -1.4 2,567,663 + Psest_2410 0.87 +0.4 2,567,664 - Psest_2410 0.87 -1.0 2,567,664 - Psest_2410 0.87 +0.2 2,567,853 + +1.6 2,567,854 - -0.6 2,568,170 + +2.3 2,568,170 + -1.6 2,568,170 + -1.8 2,568,170 + +1.0 2,568,170 + -3.1 2,568,170 + +1.7 2,568,170 + -1.8 2,568,170 + +1.1 2,568,170 + -1.5 2,568,171 - +0.4 2,568,171 - +1.4 2,568,171 - -0.3 2,568,171 - -0.7 2,568,171 - -0.6 2,568,171 - -1.7 2,568,171 - -0.1 2,568,171 - -0.6 2,568,171 - -0.5 2,568,171 - +1.9 2,568,171 - +0.4 2,568,171 - +1.1 2,568,171 - +0.4 2,568,171 - +1.5 2,568,171 - -2.1 2,568,171 - +0.1 2,568,171 - +1.2 2,568,171 - +0.9 2,568,343 + +1.5 2,568,343 + -0.3 2,568,343 + -1.3 2,568,343 + -0.6 2,568,742 + Psest_2411 0.21 -1.0 2,568,743 - Psest_2411 0.21 -0.0
Or see this region's nucleotide sequence