Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2304 and Psest_2305 are separated by 6 nucleotides Psest_2305 and Psest_2306 overlap by 4 nucleotides Psest_2306 and Psest_2307 are separated by 132 nucleotides
Psest_2304: Psest_2304 - Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family, at 2,455,315 to 2,455,809
_2304
Psest_2305: Psest_2305 - UDP-2,3-diacylglucosamine hydrolase, at 2,455,816 to 2,456,538
_2305
Psest_2306: Psest_2306 - Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA, at 2,456,535 to 2,457,185
_2306
Psest_2307: Psest_2307 - Universal stress protein UspA and related nucleotide-binding proteins, at 2,457,318 to 2,458,178
_2307
Position (kb)
2456
2457
2458 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2456.536 kb on + strand at 2456.537 kb on - strand at 2456.659 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.674 kb on + strand, within Psest_2306 at 2456.675 kb on - strand, within Psest_2306 at 2456.717 kb on - strand, within Psest_2306 at 2456.727 kb on + strand, within Psest_2306 at 2456.728 kb on - strand, within Psest_2306 at 2456.728 kb on - strand, within Psest_2306 at 2456.988 kb on + strand, within Psest_2306 at 2456.988 kb on + strand, within Psest_2306 at 2456.989 kb on - strand, within Psest_2306 at 2456.989 kb on - strand, within Psest_2306 at 2456.989 kb on - strand, within Psest_2306 at 2456.989 kb on - strand, within Psest_2306 at 2457.060 kb on + strand, within Psest_2306 at 2457.060 kb on + strand, within Psest_2306 at 2457.060 kb on + strand, within Psest_2306 at 2457.060 kb on + strand, within Psest_2306 at 2457.060 kb on + strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.061 kb on - strand, within Psest_2306 at 2457.170 kb on - strand at 2457.170 kb on - strand at 2457.214 kb on + strand at 2457.214 kb on + strand at 2457.214 kb on + strand at 2457.214 kb on + strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.215 kb on - strand at 2457.251 kb on + strand at 2457.251 kb on + strand at 2457.251 kb on + strand at 2457.252 kb on - strand at 2457.252 kb on - strand at 2457.252 kb on - strand at 2457.252 kb on - strand at 2457.252 kb on - strand at 2457.252 kb on - strand at 2457.281 kb on - strand at 2457.281 kb on - strand at 2457.282 kb on + strand at 2457.282 kb on + strand at 2457.283 kb on - strand at 2457.283 kb on - strand at 2457.283 kb on - strand at 2457.686 kb on + strand, within Psest_2307 at 2457.686 kb on + strand, within Psest_2307 at 2457.687 kb on - strand, within Psest_2307 at 2457.829 kb on + strand, within Psest_2307 at 2457.829 kb on + strand, within Psest_2307 at 2457.829 kb on + strand, within Psest_2307 at 2457.884 kb on - strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.961 kb on + strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.962 kb on - strand, within Psest_2307 at 2457.965 kb on + strand, within Psest_2307 at 2457.966 kb on - strand, within Psest_2307 at 2458.016 kb on + strand, within Psest_2307 at 2458.017 kb on - strand, within Psest_2307 at 2458.036 kb on + strand, within Psest_2307 at 2458.037 kb on - strand, within Psest_2307 at 2458.082 kb on + strand, within Psest_2307
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 2,456,536 + -1.6 2,456,537 - -1.9 2,456,659 + Psest_2306 0.19 -2.9 2,456,674 + Psest_2306 0.21 -1.5 2,456,674 + Psest_2306 0.21 -1.7 2,456,674 + Psest_2306 0.21 -0.7 2,456,675 - Psest_2306 0.22 +0.9 2,456,717 - Psest_2306 0.28 +0.2 2,456,727 + Psest_2306 0.29 -0.6 2,456,728 - Psest_2306 0.30 +2.0 2,456,728 - Psest_2306 0.30 -0.5 2,456,988 + Psest_2306 0.70 -1.1 2,456,988 + Psest_2306 0.70 -0.6 2,456,989 - Psest_2306 0.70 -1.0 2,456,989 - Psest_2306 0.70 -0.0 2,456,989 - Psest_2306 0.70 +4.1 2,456,989 - Psest_2306 0.70 -2.9 2,457,060 + Psest_2306 0.81 +0.1 2,457,060 + Psest_2306 0.81 -0.2 2,457,060 + Psest_2306 0.81 +0.1 2,457,060 + Psest_2306 0.81 -0.7 2,457,060 + Psest_2306 0.81 -0.5 2,457,061 - Psest_2306 0.81 -1.7 2,457,061 - Psest_2306 0.81 -0.1 2,457,061 - Psest_2306 0.81 -0.6 2,457,061 - Psest_2306 0.81 -5.2 2,457,061 - Psest_2306 0.81 +1.4 2,457,061 - Psest_2306 0.81 -1.7 2,457,061 - Psest_2306 0.81 -1.6 2,457,061 - Psest_2306 0.81 +0.0 2,457,061 - Psest_2306 0.81 +1.6 2,457,061 - Psest_2306 0.81 -1.9 2,457,061 - Psest_2306 0.81 -1.2 2,457,170 - -0.6 2,457,170 - -1.6 2,457,214 + +2.2 2,457,214 + -3.0 2,457,214 + +1.0 2,457,214 + +1.8 2,457,215 - -0.6 2,457,215 - +0.4 2,457,215 - -0.2 2,457,215 - +0.8 2,457,215 - +2.0 2,457,215 - +1.5 2,457,215 - +1.5 2,457,215 - -0.2 2,457,251 + -1.8 2,457,251 + -1.3 2,457,251 + -1.1 2,457,252 - +1.5 2,457,252 - -0.6 2,457,252 - -0.0 2,457,252 - -2.9 2,457,252 - +0.9 2,457,252 - -2.0 2,457,281 - +0.3 2,457,281 - -1.8 2,457,282 + +0.2 2,457,282 + -1.4 2,457,283 - -2.2 2,457,283 - +1.3 2,457,283 - -0.6 2,457,686 + Psest_2307 0.43 +0.1 2,457,686 + Psest_2307 0.43 -0.4 2,457,687 - Psest_2307 0.43 -0.8 2,457,829 + Psest_2307 0.59 -0.0 2,457,829 + Psest_2307 0.59 +1.0 2,457,829 + Psest_2307 0.59 +1.2 2,457,884 - Psest_2307 0.66 +2.4 2,457,961 + Psest_2307 0.75 -1.5 2,457,961 + Psest_2307 0.75 +0.3 2,457,961 + Psest_2307 0.75 +2.4 2,457,961 + Psest_2307 0.75 -2.3 2,457,961 + Psest_2307 0.75 -0.1 2,457,961 + Psest_2307 0.75 -0.4 2,457,961 + Psest_2307 0.75 -1.6 2,457,962 - Psest_2307 0.75 +0.0 2,457,962 - Psest_2307 0.75 +0.4 2,457,962 - Psest_2307 0.75 +1.9 2,457,962 - Psest_2307 0.75 +0.2 2,457,962 - Psest_2307 0.75 +0.6 2,457,962 - Psest_2307 0.75 +0.4 2,457,962 - Psest_2307 0.75 +0.7 2,457,965 + Psest_2307 0.75 +3.1 2,457,966 - Psest_2307 0.75 +1.1 2,458,016 + Psest_2307 0.81 +1.4 2,458,017 - Psest_2307 0.81 -0.1 2,458,036 + Psest_2307 0.83 +0.4 2,458,037 - Psest_2307 0.84 -0.2 2,458,082 + Psest_2307 0.89 +0.4
Or see this region's nucleotide sequence